Analysis Tools
mortality/aging
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• fatalities are seen in mutants older than 3 months of age that develop bacterial dermatitis
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hematopoietic system
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• leukocyte rolling along mesenteric venules is virtually absent in these mice
• TNF pre-treatment leads to a slight increase in rolling though the rolling flux is 46-fold less than in controls
• the velocity of the rare leukocyte that does bind is much greater than those in wld-type
• the number of adhering leukocytes is 6-fold less than controls
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• splenic weights are increased about 2-fold
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• spleens contain 2-fold more cells
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• differentiating myeloid precursors are significantly more numerous, however blast forms are not increased, and precursor cells from the erythroid lineage are less numerous in the bone marrow, resulting in a myeloid to erythroid ratio that is increased more than 5-fold
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• spleens contain increased numbers of CFU-GM
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• erythropoiesis is partially translocated to the spleen
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• myeloid precursor are more numerous in the bone marrow
• the myeloid to erythroid ratio is increased more than 5-fold in these mice
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• neutrophilic granulocytes compromise most of the cells of the bone marrow with an average of 60% of cells containing the segmented bands and rings morphology as compared to 42% in wild-type mice
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• increased extramedullary hematopoietic activity in the spleen
• 3 of 10 show evidence of extramedullary hematopoietic activity in the liver
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• precursor cells of the erythroid lineage are under represented in the bone marrow
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• the number of BFU-E in the spleen are significantly increased
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• there is a 3.9-fold elevation in the mean number of leukocytes in the blood
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• eosinophil numbers are increased 6.3-fold
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• more than 16-fold increase in mature polymorphonuclear neutrophils
• neonates exhibit a 3.3-fold increase in neutrophil numbers
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• lymphocyte numbers are increased 1.5-fold
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• monocyte numbers are increased 9.8-fold in the blood
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• expanded red pulp
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• neutrophil recruitment in an induced peritonitis model is reduced
• there is a five-fold impairment in the number of neutrophils recruited by thioglycollate injection
• fewer than expected neutrophils are found at the site of skin lesions
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immune system
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• leukocyte rolling along mesenteric venules is virtually absent in these mice
• TNF pre-treatment leads to a slight increase in rolling though the rolling flux is 46-fold less than in controls
• the velocity of the rare leukocyte that does bind is much greater than those in wld-type
• the number of adhering leukocytes is 6-fold less than controls
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• splenic weights are increased about 2-fold
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• spleens contain 2-fold more cells
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• myeloid precursor are more numerous in the bone marrow
• the myeloid to erythroid ratio is increased more than 5-fold in these mice
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• neutrophilic granulocytes compromise most of the cells of the bone marrow with an average of 60% of cells containing the segmented bands and rings morphology as compared to 42% in wild-type mice
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• there is a 3.9-fold elevation in the mean number of leukocytes in the blood
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• eosinophil numbers are increased 6.3-fold
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• more than 16-fold increase in mature polymorphonuclear neutrophils
• neonates exhibit a 3.3-fold increase in neutrophil numbers
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• lymphocyte numbers are increased 1.5-fold
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• monocyte numbers are increased 9.8-fold in the blood
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• expanded red pulp
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• neutrophil recruitment in an induced peritonitis model is reduced
• there is a five-fold impairment in the number of neutrophils recruited by thioglycollate injection
• fewer than expected neutrophils are found at the site of skin lesions
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• exhibit a 5-fold elevation in GM-CSF levels
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• mice have 40-fold higher levels of circulating IL-3 than controls
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• show a high prevalence of spontaneous bacterial dermatitis with or without ulceration, with the majority of mutants 4 months or older developing dermatitis
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homeostasis/metabolism
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• exhibit a 5-fold elevation in GM-CSF levels
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• mice have 40-fold higher levels of circulating IL-3 than controls
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cellular
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• neutrophilic granulocytes compromise most of the cells of the bone marrow with an average of 60% of cells containing the segmented bands and rings morphology as compared to 42% in wild-type mice
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• leukocyte rolling along mesenteric venules is virtually absent in these mice
• TNF pre-treatment leads to a slight increase in rolling though the rolling flux is 46-fold less than in controls
• the velocity of the rare leukocyte that does bind is much greater than those in wld-type
• the number of adhering leukocytes is 6-fold less than controls
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growth/size/body
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• splenic weights are increased about 2-fold
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• spleens contain 2-fold more cells
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