Phenotypes associated with this allele

• Allele Symbol: Ucp1^tm1Kz
• Allele Name: targeted mutation 1, Leslie Kozak
• Allele ID: MGI:1857471
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ucp1^tm1Kz/Ucp1^tm1Kz	129S1.Cg-Ucp^tm1Kz	MGI:2654127
hm2	Ucp1^tm1Kz/Ucp1^tm1Kz	(129S1.Cg-Ucp^tm1Kz x B6.Cg-Ucp^tm1Kz)F1	MGI:3575507
hm3	Ucp1^tm1Kz/Ucp1^tm1Kz	B6.Cg-Ucp^tm1Kz	MGI:2654128
hm4	Ucp1^tm1Kz/Ucp1^tm1Kz	involves: 129S2/SvPas	MGI:2654126
hm5	Ucp1^tm1Kz/Ucp1^tm1Kz	involves: 129S2/SvPas * C57BL/6	MGI:7606180
hm6	Ucp1^tm1Kz/Ucp1^tm1Kz	involves: 129S2/SvPas * C57BL/6J	MGI:2654130
cx7	Ucp1^tm1Kz/Ucp1^tm1Kz Ucp3^tm1Rei/Ucp3^tm1Rei	involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss	MGI:3852056
cx8	Lep^ob/Lep^ob Ucp1^tm1Kz/Ucp1^tm1Kz	involves: 129S2/SvPas * C57BL/6	MGI:4429459
cx9	Sln^tm1Peri/Sln^tm1Peri Ucp1^tm1Kz/Ucp1^tm1Kz	involves: 129S2/SvPas * C57BL/6J	MGI:6104243

Genotype
MGI:2654127

hm1

• Allelic Composition: Ucp1^tm1Kz/Ucp1^tm1Kz
• Genetic Background: 129S1.Cg-Ucp^tm1Kz

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

increased brown adipose tissue amount ( J:69573 )

• increased brown adipose tissue depot weight

cellular

abnormal mitochondrial physiology ( J:69573 )

• mitochondria exhibit an impaired response to free fatty acid-induced decoupling to a de-energized state; linoleic acid, lauric acid and stearic acid stimulation of mitochondria showed reduced rates of de-energization compared to controls as measured by protonmotive force and oxygen consumption

• comparable content of mitochondrial protein

• decreased cytochrome c oxidase activity

• increased mitochondrial glycerol-3-phosphate dehydrogenase activity

homeostasis/metabolism

impaired adaptive thermogenesis ( J:69573 )

• lost body core temperature rapidly upon exposure to cold temperatures

Genotype
MGI:3575507

hm2

• Allelic Composition: Ucp1^tm1Kz/Ucp1^tm1Kz
• Genetic Background: (129S1.Cg-Ucp^tm1Kz x B6.Cg-Ucp^tm1Kz)F1

cellular

abnormal mitochondrial physiology ( J:69573 )

• mitochondria exhibit an impaired response to free fatty acid-induced decoupling to a de-energized state; linoleic acid, lauric acid and stearic acid stimulation of mitochondria showed reduced rates of de-energization compared to controls as measured by protonmotive force and oxygen consumption

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:69573 )

N • maintained normal body temperature upon exposure to cold

Genotype
MGI:2654128

hm3

• Allelic Composition: Ucp1^tm1Kz/Ucp1^tm1Kz
• Genetic Background: B6.Cg-Ucp^tm1Kz

adipose tissue

increased brown adipose tissue amount ( J:81820 )

• an increase in the number of brown adipocytes in the inguinal fat pad was observed at at 20^oC on a high-fat diet

decreased white adipose tissue amount ( J:81820 )

• reduced weight of all white fat depots after a high-fat diet and exposure to 20^oC temperatures for 8 weeks

cellular

abnormal mitochondrial physiology ( J:69573 )

• mitochondria exhibit an impaired response to free fatty acid-induced decoupling to a de-energized state; linoleic acid, lauric acid and stearic acid stimulation of mitochondria showed reduced rates of de-energization compared to controls as measured by protonmotive force and oxygen consumption

growth/size/body

decreased body weight ( J:81820 )

• temperature dependent; at 20^oC, animals did not gain weight as quickly as controls on a high-fat, high-sucrose diet, but at 27^oC, the differences in body weight between mutants and controls were indistiguishable

• both groups of animals consumed similar amounts of food, indicating that the differences in body weight was not due to differences in energy intake

decreased susceptibility to diet-induced obesity ( J:81820 )

• at 20^oC on a high-fat diet

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:81820 )

• at 20^oC on a high-fat diet

impaired adaptive thermogenesis ( J:69573 )

• lost body core temperature rapidly upon exposure to cold temperatures

decreased circulating thyroxine level ( J:81820 )

• T4 leves were decreased

increased circulating triiodothyronine level ( J:81820 )

• T3 levels were increased

increased body temperature ( J:81820 )

• 0.1-0.3^oC higher than controls

decreased respiratory quotient ( J:81820 )

• at 20^oC, the respiratory quotient (the ratio of the volume of CO2 produced to the volume of O2 consumed) is slightly reduced

abnormal lipid homeostasis ( J:81820 )

• blood levels of beta-hydroxybuterate were significantly higher in homozygous mice suggesting higher fat oxidation rates

decreased circulating free fatty acids level ( J:81820 )

• mice have reduced levels of plasma fatty acids relative to littermates at 20^oC

decreased circulating triglyceride level ( J:81820 )

• reduced levels in bloob

Genotype
MGI:2654126

hm4

• Allelic Composition: Ucp1^tm1Kz/Ucp1^tm1Kz
• Genetic Background: involves: 129S2/SvPas

adipose tissue

increased brown adipose tissue amount ( J:40053 )

• mass of brown fat was greater in homozygous mice than in controls; thought to be due to increased deposition of triglyceride

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:40053 )

• animals did not become obese on either a low-fat or a high-fat diet

• no changes were observed in the body mass, selected fat-pad mass or in total body triglyceride content

• normal food intake was observed

impaired adaptive thermogenesis ( J:40053 )

• increased sensitivity to cold temperatures; most homozygous mice could not sustain body temperature when exposed to cold

abnormal oxygen consumption ( J:40053 )

• reduced oxygen consumption compared to controls was observed after treatment with a beta3-adrenergic agonist; resting oxygen consuption levels were similar to controls

decreased oxygen consumption ( J:62312 )

• in CL316243-treated mice

abnormal physiological response to xenobiotic ( J:40053 )

• abnormal response to beta3-adrenergic stimulation by the CL 316,243 agonist

growth/size/body

decreased susceptibility to diet-induced obesity ( J:40053 )

• animals did not become obese on either a low-fat or a high-fat diet

• no changes were observed in the body mass, selected fat-pad mass or in total body triglyceride content

• normal food intake was observed

Genotype
MGI:7606180

hm5

• Allelic Composition: Ucp1^tm1Kz/Ucp1^tm1Kz
• Genetic Background: involves: 129S2/SvPas * C57BL/6

homeostasis/metabolism

impaired febrile response ( J:345254 )

• prostaglandin E2 (PGE2)-induced thermogenesis is totally blunted in mice kept under a room temperature condition

Genotype
MGI:2654130

hm6

• Allelic Composition: Ucp1^tm1Kz/Ucp1^tm1Kz
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

cellular

abnormal mitochondrial physiology ( J:57998 )

• mutant mitochondria have lost the high GDP-binding capacity and do not become energized in reposnse to it; instead, mutant mitochondria are in a perpetually energized state

• mitochondria do respond to free fatty acids by uncoupling to a de-energized state similar to controls

Genotype
MGI:3852056

cx7

• Allelic Composition: Ucp1^tm1Kz/Ucp1^tm1Kz; Ucp3^tm1Rei/Ucp3^tm1Rei
• Genetic Background: involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss

homeostasis/metabolism

decreased oxygen consumption ( J:62312 )

• similar to in Ucp1^tm1Kz homozygotes, the respiratory exchange ratio of CL316243-treated mice is less than in similarly treated wild-type mice

Genotype
MGI:4429459

cx8

• Allelic Composition: Lep^ob/Lep^ob; Ucp1^tm1Kz/Ucp1^tm1Kz
• Genetic Background: involves: 129S2/SvPas * C57BL/6

growth/size/body

weight loss ( J:129662 )

• body weight and lean mass decline as the ambient temperature drops to 12C

• fat mass is maintained

homeostasis/metabolism

impaired adaptive thermogenesis ( J:129662 )

• do not survive when the ambient temperature remains below 12C

• leptin injections enhance survival at ambient temperatures below 8C by inducing T3 production

• T3 treatment protective against cold exposure

• hypothermic by the time the ambient temperature drops to 8C

increased oxygen consumption ( J:129662 )

• oxygen consumption increases temporarily when ambient temperature drops from 28 to 21C

• after 15 days at 21C, oxygen consumption returns to 28C level

decreased respiratory quotient ( J:129662 )

• respiratory exchange ratio about 3% lower than for Lep^ob homozygotes at 21C

• respiratory exchange ratios are identical at 28C

Genotype
MGI:6104243

cx9

• Allelic Composition: Sln^tm1Peri/Sln^tm1Peri; Ucp1^tm1Kz/Ucp1^tm1Kz
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

mortality/aging

prenatal lethality, incomplete penetrance ( J:193427 )

• fewer than expected mice are born