Phenotypes associated with this allele

• Allele Symbol: Mycn^tm1Par
• Allele Name: targeted mutation 1, Luis Parada
• Allele ID: MGI:1857492
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mycn^tm1Par/Mycn^tm1Par	involves: 129S2/SvPas * C57BL/6J	MGI:3620015
ht2	Mycn^tm1Par/Mycn^+	involves: 129S2/SvPas * C57BL/6J	MGI:3620014
ht3	Mycn^tm1Jrt/Mycn^tm1Par	involves: 129S2/SvPas * C57BL/6J	MGI:3620016

Genotype
MGI:3620015

hm1

• Allelic Composition: Mycn^tm1Par/Mycn^tm1Par
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:3432 )

• homozygotes die at ~E11.5

• by E12.5, homozygotes appear highly necrotic and pale (presumably due to anemia) and show no signs of life

nervous system

abnormal embryonic neuroepithelial layer differentiation ( J:3432 )

• at E9.5, homozygotes exhibit a wavy neuroepithelium and an aberrant ectodermal surface

• by E11.5, the neuroepithelium is thin and lacks architectural complexity

abnormal forebrain development ( J:3432 )

• at E9.5, homozygotes display an abnormal prosencephalon

abnormal medulla oblongata morphology ( J:3432 )

• at E9.5, homozygotes display an abnormal roof plate in the myelencephalon

telencephalon hypoplasia ( J:3432 )

• at E11.5, homozygotes display hypoplastic telencephalic structures

abnormal cranial ganglia morphology ( J:3432 )

• by E11.5, cranial ganglia are generally reduced, with a mitotic rate of less than 25% of wild-type

small trigeminal ganglion ( J:3432 )

• by E11.5, homozygotes display lack of organization and a reduction of neurite outgrowths in the trigeminal ganglia

disorganized dorsal root ganglion ( J:3432 )

small dorsal root ganglion ( J:3432 )

• by E11.5, homozygotes display a reduction of neurite outgrowths in the dorsal root ganglia

respiratory system

abnormal lung development ( J:3432 )

• at E10.5, the mutant lung epithelium fails to undergo proper branching; as a result, mutant lungs appear as simple tube-like structures

• however, no morphologic abnormalities are noted in the mesenchymal component

impaired branching involved in bronchus morphogenesis ( J:3432 )

• at E10.5, mutant lungs fail to display branching morphogenesis

pulmonary hypoplasia ( J:3432 )

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:3432 )

• at E10.5-E11.5, homozygotes display abnormal growth and/or disorganization of the gut epithelium

abnormal large intestine morphology ( J:3432 )

• at E11.5, homozygotes lack an identifiable large intestine

absent stomach ( J:3432 )

• at E11.5, homozygotes lack an identifiable stomach

embryo

first pharyngeal arch hypoplasia ( J:3432 )

• at E9.5, homozygotes display hypoplasia of the mandibular arch, the first postoral arch in the branchial arch series

abnormal embryo development ( J:3432 )

• as early as E9.5, homozygotes display hypoplasia of several organ systems, particularly those of epithelial origin such as the lung and gut

embryonic growth arrest ( J:3432 )

• homozygotes become growth arrested at ~E11.5

embryonic growth retardation ( J:3432 )

• starting at E10.5, homozygotes exhibit progressive growth retardation

• by E11.5, homozygotes are ~40% the size of wild-type embryos

decreased embryo size ( J:3432 )

• at E9.5, homozygotes appear thinner in transverse dimension relative to wild-type embryos

gonadal ridge hypoplasia ( J:3432 )

• at E11.5, homozygotes exhibit a hypoplastic genital ridge with an irregular, hobnail-like coelomic epithelium

• both the stromal and germ cell components of the genital ridge are severly hypoplastic (~1/10 of wild-type size)

abnormal mesonephros morphology ( J:3432 )

• at E9.5, some (but not all) homozygotes exhibit a slight delay in the initial formation of the mesonephric tubules

• by E11.5, some tubules appear normal while others fail to form or undergo premature degeneration

abnormal Wolffian duct morphology ( J:3432 )

• at E9.5, some (but not all) homozygotes exhibit a slight delay in the initial formation of the mesonephric duct

abnormal embryonic neuroepithelial layer differentiation ( J:3432 )

• at E9.5, homozygotes exhibit a wavy neuroepithelium and an aberrant ectodermal surface

• by E11.5, the neuroepithelium is thin and lacks architectural complexity

cardiovascular system

abnormal dorsal aorta morphology ( J:3432 )

dilated dorsal aorta ( J:3432 )

• at E11.5, the mutant dorsal aorta is dilated

thin myocardium ( J:3432 )

• at E11.5, homozygotes display a thinned myocardium

hemorrhage ( J:3432 )

• at E11.5, homozygotes are fragile and bleed easily, despite the presence of a beating heart

craniofacial

first pharyngeal arch hypoplasia ( J:3432 )

• at E9.5, homozygotes display hypoplasia of the mandibular arch, the first postoral arch in the branchial arch series

growth/size/body

embryonic growth retardation ( J:3432 )

• starting at E10.5, homozygotes exhibit progressive growth retardation

• by E11.5, homozygotes are ~40% the size of wild-type embryos

decreased embryo size ( J:3432 )

• at E9.5, homozygotes appear thinner in transverse dimension relative to wild-type embryos

hematopoietic system

anemia ( J:3432 )

• by E12, homozygotes appear severely anemic

renal/urinary system

abnormal mesonephric mesenchyme morphology ( J:3432 )

• the mesenchyme surrounding the mesonephric tubules is hypoplastic

muscle

thin myocardium ( J:3432 )

• at E11.5, homozygotes display a thinned myocardium

Genotype
MGI:3620014

ht2

• Allelic Composition: Mycn^tm1Par/Mycn⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

mortality/aging

postnatal lethality, incomplete penetrance ( J:3432 )

• heterozygotes are present at the expected Mendelian ratio at E18.5 but are significantly under-represented at 3 weeks

• surviving heterozygotes are fertile, healthy and exhibit no differences in size, weight, or lifespan relative to wild-type mice

Genotype
MGI:3620016

ht3

• Allelic Composition: Mycn^tm1Jrt/Mycn^tm1Par
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:15281 )

• compound heterozygotes start dying at ~E12.5, when 12 out of 42 mutants are found to be dead and necrotic

• no live compound heterozygotes are obtained after E14.5

cardiovascular system

abnormal vein morphology ( J:15281 )

• at E12.5, compound heterozygotes with an edematous neck area exhibit massively dilated jugular veins, indicating cardiac dysfunction

• however, no other major veins are shown to exhibit significant dilation

thin myocardium compact layer ( J:15281 )

• at E12.5, compound heterozygotes show a thin subepicardial layer in the ventricular myocardium; the atrial myocardium and inner trabecular layer of ventricles are less severly affected

• at E12.5, the mutant subepicardial ("compact") layer has failed to grow and is as thick as at E10.5

thin myocardium ( J:15281 )

• at E12.5, compound heterozygotes display a thinned myocardium relative to wild-type mice

small heart ( J:15281 )

abnormal fetal cardiomyocyte proliferation ( J:15281 )

• at E12.5 and E14.5, compound heterozygotes fail to exhibit proliferation in the subepicardial ("compact") layer of the ventricular myocardium

congestive heart failure ( J:15281 )

respiratory system

impaired branching involved in bronchus morphogenesis ( J:15281 )

• at E12.5, compound heterozygotes display an exacerbated branching defect in the lung epithelium relative to Mycn^tm1Jrt homozygotes, as shown by absence of tertiary branching

homeostasis/metabolism

edema ( J:15281 )

• at E12.5, 23 out of 42 compound heterozygotes display a large edema in the neck area

• all compound heterozygotes surviving to E14.5 show an edematous neck area

embryo

decreased embryo size ( J:15281 )

• at E12.5, 2 out of 42 compound heterozygotes are slightly smaller than wild-type embryos

renal/urinary system

small kidney ( J:15281 )

• compound heterozygotes that survive to E14.5 exhibit small but structurally normal kidneys relative to wild-type mice

growth/size/body

decreased embryo size ( J:15281 )

• at E12.5, 2 out of 42 compound heterozygotes are slightly smaller than wild-type embryos

decreased fetal size ( J:15281 )

• all compound heterozygotes surviving to E14.5 are smaller than wild-type embryos

muscle

thin myocardium compact layer ( J:15281 )

• at E12.5, compound heterozygotes show a thin subepicardial layer in the ventricular myocardium; the atrial myocardium and inner trabecular layer of ventricles are less severly affected

• at E12.5, the mutant subepicardial ("compact") layer has failed to grow and is as thick as at E10.5

thin myocardium ( J:15281 )

• at E12.5, compound heterozygotes display a thinned myocardium relative to wild-type mice

abnormal fetal cardiomyocyte proliferation ( J:15281 )

• at E12.5 and E14.5, compound heterozygotes fail to exhibit proliferation in the subepicardial ("compact") layer of the ventricular myocardium

nervous system

nervous system phenotype ( J:15281 )

N • unlike Mycn^tm1Par homozygotes, compound heterozygotes do NOT exhibit reduced cranial or spinal ganglia

reproductive system

reproductive system phenotype ( J:15281 )

N • unlike Mycn^tm1Par homozygotes, compound heterozygotes do NOT exhibit reduced genital ridges

cellular

abnormal fetal cardiomyocyte proliferation ( J:15281 )

• at E12.5 and E14.5, compound heterozygotes fail to exhibit proliferation in the subepicardial ("compact") layer of the ventricular myocardium