Phenotypes associated with this allele

• Allele Symbol: Klf1^tm1Nimr
• Allele Name: targeted mutation 1, National Institute of Medical Research
• Allele ID: MGI:1857557
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Klf1^tm1Nimr/Klf1^tm1Nimr	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CBA) or (involves: 129P2/OlaHsd * FVB)	MGI:3653720
cn2	Bcl11a^tm1Mbu/Bcl11a^tm1Mbu Klf1^tm1Nimr/Klf1^+ Epor^tm1.1(EGFP/icre)Uk/Epor^+ Tg(HBB)8.1Gvs/0	involves: 129P2/OlaHsd * BALB/cJ * FVB/N	MGI:5496872
cn3	Bcl11a^tm1Mbu/Bcl11a^tm1Mbu Klf1^tm1Nimr/Klf1^+ Tg(HBB)8.1Gvs/0	involves: 129P2/OlaHsd * FVB	MGI:5496870

Genotype
MGI:3653720

hm1

• Allelic Composition: Klf1^tm1Nimr/Klf1^tm1Nimr
• Genetic Background: either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CBA) or (involves: 129P2/OlaHsd * FVB)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:25650 )

• homozygotes die of rapid, fatal anemia at ~E14 after switching of embryonic hematopoiesis to the fetal liver

hematopoietic system

abnormal erythropoiesis ( J:25650 )

• homozygotes display a defect in the late steps of differentiation of mature erythroid progenitors

anemia ( J:25650 )

• by E14.5, homozygotes are severely anemic

impaired hematopoiesis ( J:25650 )

• homozygotes exhibit defective hematopoiesis in fetal liver

• enucleated erythrocytes are formed but do not contain the full complement of hemoglobin

erythroblastosis ( J:25650 )

• at E12.5, homozygotes exhibit erythroblastosis

decreased hemoglobin content ( J:25650 )

• at E14.5, mutant fetal livers contain no hemoglobin

liver/biliary system

small liver ( J:25650 )

• at E12.5, homozygotes display significant growth retardation of the fetal liver

• however, a normal number of colony-forming cells are detected in early fetal liver, indicating that the progenitor cell compartment is unaffected

pale liver ( J:25650 )

• at E12,5 and E13.5, homozygotes exhibit pale livers that lack their full complement of hemoglobin

Genotype
MGI:5496872

cn2

• Allelic Composition: Bcl11a^tm1Mbu/Bcl11a^tm1Mbu; Klf1^tm1Nimr/Klf1⁺; Epor^{tm1.1(EGFP/icre)Uk}/Epor⁺; Tg(HBB)8.1Gvs/0
• Genetic Background: involves: 129P2/OlaHsd * BALB/cJ * FVB/N

hematopoietic system

abnormal erythropoiesis ( J:196405 )

• increased Ter119+/CD71+ cells in the blood

polychromatophilia ( J:196405 )

Genotype
MGI:5496870

cn3

• Allelic Composition: Bcl11a^tm1Mbu/Bcl11a^tm1Mbu; Klf1^tm1Nimr/Klf1⁺; Tg(HBB)8.1Gvs/0
• Genetic Background: involves: 129P2/OlaHsd * FVB

mortality/aging

mortality/aging ( J:196405 )

N • mice are produced in expected Mendelian ratios

hematopoietic system

abnormal erythropoiesis ( J:196405 )

• increased CD71-/Ter119+ cells in the blood and liver at E18.5

• increased Ter119+/CD71+ cells in the blood

• increased Ter119+/DRAQ5+ cells in the blood

anemia ( J:196405 )

• mild

decreased erythrocyte cell number ( J:196405 )

• more pronounced than in Bcl11a^tm1Phi/Bcl11a^tm1Phi Epor^{tm1(EGFP/cre)Uk}/Epor⁺ Tg(HBB)8.1Gvs mice

decreased hematocrit ( J:196405 )

• more pronounced than in Bcl11a^tm1Phi/Bcl11a^tm1Phi Epor^{tm1(EGFP/cre)Uk}/Epor⁺ Tg(HBB)8.1Gvs mice

decreased hemoglobin content ( J:196405 )

• more pronounced than in Bcl11a^tm1Phi/Bcl11a^tm1Phi Epor^{tm1(EGFP/cre)Uk}/Epor⁺ Tg(HBB)8.1Gvs mice

decreased mean corpuscular hemoglobin ( J:196405 )

• small

decreased mean corpuscular volume ( J:196405 )

• small

polychromatophilia ( J:196405 )

• more so than in Bcl11a^tm1Phi/Bcl11a^tm1Phi Klf1^tm1Nimr/Klf1⁺ Tg(HBB)8.1Gvs mice

reticulocytosis ( J:196405 )

homeostasis/metabolism

increased circulating erythropoietin level ( J:196405 )

• indicating anemic stress