Phenotypes associated with this allele

• Allele Symbol: Mgat1^tm1Pst
• Allele Name: targeted mutation 1, Pamela Stanley
• Allele ID: MGI:1857564
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mgat1^tm1Pst/Mgat1^tm1Pst	involves: 129/Sv * C57BL/6 * C57BL/6J * CD-1 * SJL	MGI:2175109
cn2	Mgat1^tm2Jxm/Mgat1^tm1Pst Tg(Zp3-cre)1Gwh/0	involves: 129/Sv * C57BL/6 * SJL	MGI:3521575

Genotype
MGI:2175109

hm1

• Allelic Composition: Mgat1^tm1Pst/Mgat1^tm1Pst
• Genetic Background: involves: 129/Sv * C57BL/6 * C57BL/6J * CD-1 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:16489 )

• at E10.5

embryo

embryonic growth retardation ( J:16489 )

• at E9.5, mutant embryos most resemble stage 14 (E9.0) of control embryos

decreased embryo size ( J:16489 )

• 25% the size of controls at E10.5 with signs of degeneration

• at E9.5, embryos are small, but close to the size of the smallest controls

incomplete caudal neuropore closure ( J:16489 )

• enlarged tail area, suggesting an open posterior neuropore

incomplete rostral neuropore closure ( J:16489 )

• abnormal cephalic region; small and amorphous, with characteristic contours absent, suggesting open anterior neuropore

incomplete somite formation ( J:16489 )

• at E9.5, mutant embryos have 13-18 somites, whereas wild-type and heterozygous embryos have 22-28 somites

growth/size/body

embryonic growth retardation ( J:16489 )

• at E9.5, mutant embryos most resemble stage 14 (E9.0) of control embryos

decreased embryo size ( J:16489 )

• 25% the size of controls at E10.5 with signs of degeneration

• at E9.5, embryos are small, but close to the size of the smallest controls

integument

pallor ( J:16489 )

• embryos are pale, suggesting anemia

nervous system

incomplete caudal neuropore closure ( J:16489 )

• enlarged tail area, suggesting an open posterior neuropore

incomplete rostral neuropore closure ( J:16489 )

• abnormal cephalic region; small and amorphous, with characteristic contours absent, suggesting open anterior neuropore

cardiovascular system

hemorrhage ( J:16489 )

• at E9.5, small blood spot are seen in the head and other regions

Genotype
MGI:3521575

cn2

• Allelic Composition: Mgat1^tm2Jxm/Mgat1^tm1Pst; Tg(Zp3-cre)1Gwh/0
• Genetic Background: involves: 129/Sv * C57BL/6 * SJL

reproductive system

abnormal oogenesis ( J:94224 )

♀ • the number of eggs obtained from mutant females is about 50% that from control females lacking Tg(Zp3-cre)1Gwh and more mutant females failed to produce any eggs after superovulation

abnormal zona pellucida morphology ( J:94224 )

♀ • the zona pellucida is thinner, less rigid, more loosely attached to the egg, lacks complex N glycans, and is stickier, however mutant eggs can still be successfully fertilized

thin zona pellucida ( J:94224 )

♀

reduced female fertility ( J:94224 )

♀ • mutant females crossed to heterozygous or wild-type males have more abnormal or underdeveloped embryos compared to control females

♀ • 41% of mutant females fail to produce any embryos within 5 days of their first mating compared to about 12% of control females

decreased litter size ( J:94224 )

• average litter size for mutant females mated to wild-type males is only 3.8 pups compared to 7.1 pups for wild-type females

cellular

abnormal oogenesis ( J:94224 )

♀ • the number of eggs obtained from mutant females is about 50% that from control females lacking Tg(Zp3-cre)1Gwh and more mutant females failed to produce any eggs after superovulation

abnormal zona pellucida morphology ( J:94224 )

♀ • the zona pellucida is thinner, less rigid, more loosely attached to the egg, lacks complex N glycans, and is stickier, however mutant eggs can still be successfully fertilized

thin zona pellucida ( J:94224 )

♀