Phenotypes associated with this allele

• Allele Symbol: Rad51^tm1Hst
• Allele Name: targeted mutation 1, Paul Hasty
• Allele ID: MGI:1857587
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rad51^tm1Hst/Rad51^tm1Hst	involves: 129S7/SvEvBrd * C57BL/6	MGI:2669562
cx2	Rad51^tm1Hst/Rad51^tm1Hst Trp53^tm1Brd/Trp53^tm1Brd	involves: 129S7/SvEvBrd * C57BL/6	MGI:3795437

Genotype
MGI:2669562

hm1

• Allelic Composition: Rad51^tm1Hst/Rad51^tm1Hst
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:36540 )

• embryos die between E5.5 to E7.5

• at E7.5, embryos are beginning to resorb

embryo

absent inner cell mass proliferation ( J:36540 )

• mutant cells derived from the inner cell mass failed to proliferate in vitro

embryonic growth arrest ( J:36540 )

• growth arrest at the egg cylinder stage (E5.5)

decreased embryo size ( J:36540 )

• at E7.5, embryos are smaller than controls

abnormal embryonic tissue morphology ( J:36540 )

• homozygous embryos are described as hypocellular with cuboidal cell morphology

increased embryonic epiblast cell apoptosis ( J:36540 )

• apoptotic cells are frequently observed in epiblasts of E7.5 mutant embryos

absent mesoderm ( J:36540 )

absent amniotic cavity ( J:36540 )

• amniotic cavity fails to develop

growth/size/body

decreased embryo size ( J:36540 )

• at E7.5, embryos are smaller than controls

cellular

increased cellular sensitivity to gamma-irradiation ( J:36540 )

• trophoblast cells in culture are reduced in number and fewer cells attach to culture plates after exposure to gamma irradiation

increased embryonic epiblast cell apoptosis ( J:36540 )

• apoptotic cells are frequently observed in epiblasts of E7.5 mutant embryos

decreased cell proliferation ( J:36540 )

• mutant embryos incorporate less BrdU at E5.5 and E6.5 indicated reduced cellular proliferation

absent inner cell mass proliferation ( J:36540 )

• mutant cells derived from the inner cell mass failed to proliferate in vitro

Genotype
MGI:3795437

cx2

• Allelic Composition: Rad51^tm1Hst/Rad51^tm1Hst; Trp53^tm1Brd/Trp53^tm1Brd
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:36540 )

• double mutant embryos survive longer than Rad51^tm1Hst homozygous mice; embryos appear grossly normal at E7.5, but are smaller at E8.5 and do not develop further

cellular

decreased cell proliferation ( J:36540 )

• MEFs derived from double-homozygous mice do not proliferate in culture and exhibit characteristics of cells entering premature senescence