Phenotypes associated with this allele

• Allele Symbol: Ctnnb1^tm1Max
• Allele Name: targeted mutation 1, Max Planck Institut fur Immunbiologie
• Allele ID: MGI:1857678
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ctnnb1^tm1Max/Ctnnb1^tm1Max	involves: 129 * 129S1/Sv * 129X1/SvJ	MGI:2168872
cn2	Ctnnb1^tm1Max/Ctnnb1^tm2Kem Hesx1^tm1(cre)Jpmb/Hesx1^tm1Icar	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ	MGI:5314536
cn3	Ctnnb1^tm1Max/Ctnnb1^tm2Kem Tg(Msx2-cre)5Rem/0	involves: 129S1/Sv * 129X1/SvJ	MGI:2450904
cn4	Ctnnb1^tm1Max/Ctnnb1^tm2Kem Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129S1/Sv * 129X1/SvJ * CD-1	MGI:3689415
cx5	Ctnnb1^tm1Max/Ctnnb1^+ Hesx1^tm1Icar/Hesx1^tm1Icar	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ	MGI:5314538
cx6	Ctnnb1^tm1Max/Ctnnb1^+ Hesx1^tm1(cre)Jpmb/Hesx1^tm1Icar	involves: 129P2/OlaHsd * 129S/SvEv * 129S1/Sv * 129X1/SvJ	MGI:5314535

Genotype
MGI:2168872

hm1

• Allelic Composition: Ctnnb1^tm1Max/Ctnnb1^tm1Max
• Genetic Background: involves: 129 * 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:29881 )

• no homozygous mutants born from crosses of heterozygotes

• development seemed normal through E7

• by E8.5 cell death could be observed in some areas

• resorption continuing at E9.5 when most organized structure disappeared

embryo

abnormal germ layer development ( J:29881 )

• the 3 germ layers fail to form

decreased embryo size ( J:29881 )

• by E7.5 homozygotes were about half normal length

abnormal embryonic epiblast morphology ( J:29881 )

• around E7.0 cells begin detaching from the embryonic ectoderm and disperse into the proamniotic cavity

abnormal extraembryonic tissue morphology ( J:29881 )

• extraembryonic cavities failed to form

absent allantois ( J:29881 )

• allantois failed to form

absent amniotic folds ( J:29881 )

growth/size/body

decreased embryo size ( J:29881 )

• by E7.5 homozygotes were about half normal length

Genotype
MGI:5314536

cn2

• Allelic Composition: Ctnnb1^tm1Max/Ctnnb1^tm2Kem; Hesx1^tm1(cre)Jpmb/Hesx1^tm1Icar
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ

vision/eye

microphthalmia ( J:181005 )

• unilateral in some mice

• bilateral in some mice

anophthalmia ( J:181005 )

• unilateral in some mice

nervous system

small embryonic telencephalon ( J:181005 )

• in some mice

Genotype
MGI:2450904

cn3

• Allelic Composition: Ctnnb1^tm1Max/Ctnnb1^tm2Kem; Tg(Msx2-cre)5Rem/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

neonatal lethality, complete penetrance ( J:81795 )

• mutant mice are live-born but die within 24 hrs of birth

limbs/digits/tail

abnormal apical ectodermal ridge morphology ( J:81795 )

• AER formation fails to initiate in the mutant hindlimbs whereas in the forelimbs, AER is properly initiated at 20 somites but eventually disappears in the anterior and posterior extremes by ~38 somites

abnormal forelimb morphology ( J:81795 )

• mutant forelimbs are present but truncated at the level of the humerus or ulna

abnormal humerus morphology ( J:81795 )

absent deltoid tuberosity ( J:81795 )

• in newborn mutants, the humerus appears largely unaffected but shows absence of the deltoid crest

abnormal ulna morphology ( J:81795 )

• in mutant newborns, the proximal ulna is present to variable extents

absent hindlimb ( J:81795 )

• all mutant pups completely lack hindlimbs

skeleton

abnormal humerus morphology ( J:81795 )

absent deltoid tuberosity ( J:81795 )

• in newborn mutants, the humerus appears largely unaffected but shows absence of the deltoid crest

abnormal ulna morphology ( J:81795 )

• in mutant newborns, the proximal ulna is present to variable extents

embryo

increased ectoderm apoptosis ( J:81795 )

• in mutant hindlimbs, where an AER never forms, extensive apoptosis occurs throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally

• in the forelimbs, however, where the AER disappears later in development, apoptosis is restricted to the distal mesenchyme and ectoderm

• no significant reduction of cell proliferation is observed in the mutant mesenchyme or ectoderm

abnormal apical ectodermal ridge morphology ( J:81795 )

• AER formation fails to initiate in the mutant hindlimbs whereas in the forelimbs, AER is properly initiated at 20 somites but eventually disappears in the anterior and posterior extremes by ~38 somites

behavior/neurological

abnormal suckling behavior ( J:81795 )

• newborn mutant mice fail to nurse

cellular

increased ectoderm apoptosis ( J:81795 )

• in mutant hindlimbs, where an AER never forms, extensive apoptosis occurs throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally

• in the forelimbs, however, where the AER disappears later in development, apoptosis is restricted to the distal mesenchyme and ectoderm

• no significant reduction of cell proliferation is observed in the mutant mesenchyme or ectoderm

Genotype
MGI:3689415

cn4

• Allelic Composition: Ctnnb1^tm1Max/Ctnnb1^tm2Kem; Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CD-1

limbs/digits/tail

abnormal limb development ( J:114494 )

• at E16.5, clear zones of hypertrophy are visible in limbs compared to controls

skeleton

arrested osteoblast differentiation ( J:114494 )

• in mutant tibia, no Oc+ osteoblasts are detected, indicating failure of osteoblast progression to terminal osteoblasts

abnormal cartilage morphology ( J:114494 )

• hypertrophic chondrocytes line the periosteal region in addition to the normal growth plate

abnormal bone ossification ( J:114494 )

• membranous bone cranial ossification centers are absent at E18.5

• ossification in mutant periosteum of the tibia is absent

abnormal bone mineralization ( J:114494 )

• at E18.5, mutant limbs show absence of mineralized bone matrix compared to wild-type embryos and remaining mineralization is associated with hypertrophic chondrocytes; there appears to be complete loss of bone deposition

cellular

arrested osteoblast differentiation ( J:114494 )

• in mutant tibia, no Oc+ osteoblasts are detected, indicating failure of osteoblast progression to terminal osteoblasts

Genotype
MGI:5314538

cx5

• Allelic Composition: Ctnnb1^tm1Max/Ctnnb1⁺; Hesx1^tm1Icar/Hesx1^tm1Icar
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ

nervous system

abnormal forebrain development ( J:181005 )

small embryonic telencephalon ( J:181005 )

• in some mice

vision/eye

microphthalmia ( J:181005 )

• bilateral in some mice

• unilateral in some mice

anophthalmia ( J:181005 )

• unilateral in some mice

Genotype
MGI:5314535

cx6

• Allelic Composition: Ctnnb1^tm1Max/Ctnnb1⁺; Hesx1^tm1(cre)Jpmb/Hesx1^tm1Icar
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * 129S1/Sv * 129X1/SvJ

nervous system

abnormal forebrain development ( J:181005 )

small embryonic telencephalon ( J:181005 )

• in some mice

vision/eye

microphthalmia ( J:181005 )

• bilateral in some mice

• unilateral in some mice

anophthalmia ( J:181005 )

• unilateral in some mice