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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Smad4tm1Cxd
targeted mutation 1, Chu-Xia Deng
MGI:1857698
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Smad4tm1Cxd/Smad4tm1Cxd involves: 129S6/SvEvTac MGI:2182722
ht2
Smad4tm1Cxd/Smad4+ involves: 129S6/SvEvTac MGI:3653350
ht3
Smad4m1Mag/Smad4tm1Cxd either: (involves: 129S/Sv * 129S6/SvEvTac * Black Swiss) or (involves: 129S/Sv * 129S6/SvEvTac * C57BL/6) MGI:3713867
ht4
Smad4m2Mag/Smad4tm1Cxd either: (involves: 129S/Sv * 129S6/SvEvTac * Black Swiss) or (involves: 129S/Sv * 129S6/SvEvTac * C57BL/6) MGI:3713868
ht5
Smad4m3Mag/Smad4tm1Cxd either: (involves: 129S/Sv * 129S6/SvEvTac * Black Swiss) or (involves: 129S/Sv * 129S6/SvEvTac * C57BL/6) MGI:3713870
ht6
Smad4m4Mag/Smad4tm1Cxd either: (involves: 129S/Sv * 129S6/SvEvTac * Black Swiss) or (involves: 129S/Sv * 129S6/SvEvTac * C57BL/6) MGI:3713871


Genotype
MGI:2182722
hm1
Allelic
Composition
Smad4tm1Cxd/Smad4tm1Cxd
Genetic
Background
involves: 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad4tm1Cxd mutation (0 available); any Smad4 mutation (47 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Morphological and molecular analyses of Smad4tm1Cxd/Smad4tm1Cxd embryos

mortality/aging
• at E8.5-E9.5, a small portion of homozygotes are still viable and exhibit an increased size relative to E6.5-E7.5 mutants
• at E8.5, some abnormal embryos resemble small solid balls while others show abnormally enlarged sac-like structures with presumptive embryonic endoderm and ectoderm

embryo
• after 5 days in culture, the ICM outgrowths of mutant embryos are only ~25%-50% the size of wild-type embryos
• in contrast to wild-type, none of the mutant ICM outgrowths display any signs of endoderm differentiation after 7 days in culture
• homozygotes are developmentally arrested at the onset of gastrulation, although some are still alive beyond E6.5
• little elongation in the extraembryonic portion of the late egg cylinder is observed
• at E6.5, homozygotes are only 25%-50% the size of wild-type or heterozygous embryos
• homozygotes remain significantly smaller than wild-type embryos at E7.5
• at E6.5, homozygotes display reduced epiblast proliferation relative to wild-type embryos
• at E6.5 and E7.5, homozygotes display either absent or significantly reduced and distorted extraembryonic egg cylinders; at E7.5, both types of abnormal embryos show larger embryonic portions than mutants at E6.5
• although a portion of mutant embryos at E8.5 display an increase in embryonic ectoderm and endoderm, they fail to form mesoderm
• at E7.5, homozygotes lack an identifiable primitive streak and headfold
• at E6.5, homozygotes display no obvious boundary between the embryonic and extraembryonic portions
• at E7.5, homozygotes exhibit a significantly reduced extraembryonic portion relative to E6.5 and E7.5 wild-type embryos
• at E7.5, homozygotes remain small but display a relatively large Reichert's membrane

growth/size/body
• at E6.5, homozygotes are only 25%-50% the size of wild-type or heterozygous embryos
• homozygotes remain significantly smaller than wild-type embryos at E7.5

cellular
• at E6.5, homozygotes exhibit significantly reduced epiblast cell proliferation, as shown by weak PCNA staining
• after 5 days in culture, the ICM outgrowths of mutant embryos are only ~25%-50% the size of wild-type embryos
• at E6.5, homozygotes display reduced epiblast proliferation relative to wild-type embryos




Genotype
MGI:3653350
ht2
Allelic
Composition
Smad4tm1Cxd/Smad4+
Genetic
Background
involves: 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad4tm1Cxd mutation (0 available); any Smad4 mutation (47 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• heterozygotes appear normal in growth, health, and fertility with no detectable tumor formation up to 8 months of age




Genotype
MGI:3713867
ht3
Allelic
Composition
Smad4m1Mag/Smad4tm1Cxd
Genetic
Background
either: (involves: 129S/Sv * 129S6/SvEvTac * Black Swiss) or (involves: 129S/Sv * 129S6/SvEvTac * C57BL/6)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad4m1Mag mutation (0 available); any Smad4 mutation (47 available)
Smad4tm1Cxd mutation (0 available); any Smad4 mutation (47 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice show no overt phenotype up to at least 5 months of age




Genotype
MGI:3713868
ht4
Allelic
Composition
Smad4m2Mag/Smad4tm1Cxd
Genetic
Background
either: (involves: 129S/Sv * 129S6/SvEvTac * Black Swiss) or (involves: 129S/Sv * 129S6/SvEvTac * C57BL/6)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad4m2Mag mutation (0 available); any Smad4 mutation (47 available)
Smad4tm1Cxd mutation (0 available); any Smad4 mutation (47 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice show no overt phenotype up to at least 5 months of age




Genotype
MGI:3713870
ht5
Allelic
Composition
Smad4m3Mag/Smad4tm1Cxd
Genetic
Background
either: (involves: 129S/Sv * 129S6/SvEvTac * Black Swiss) or (involves: 129S/Sv * 129S6/SvEvTac * C57BL/6)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad4m3Mag mutation (0 available); any Smad4 mutation (47 available)
Smad4tm1Cxd mutation (0 available); any Smad4 mutation (47 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice show no overt phenotype up to at least 5 months of age




Genotype
MGI:3713871
ht6
Allelic
Composition
Smad4m4Mag/Smad4tm1Cxd
Genetic
Background
either: (involves: 129S/Sv * 129S6/SvEvTac * Black Swiss) or (involves: 129S/Sv * 129S6/SvEvTac * C57BL/6)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad4m4Mag mutation (0 available); any Smad4 mutation (47 available)
Smad4tm1Cxd mutation (0 available); any Smad4 mutation (47 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• embryos do not survive embryogenesis, and display defects not compatible with life aound ~E7.5

embryo
• at E7.5, animals are severely growth retarded
• embryos do not form mesoderm due to failure of gastrulation

growth/size/body
• at E7.5, animals are severely growth retarded





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory