Phenotypes associated with this allele

• Allele Symbol: Nrp1^tm1Hfu
• Allele Name: targeted mutation 1, Hajime Fujisawa
• Allele ID: MGI:1857853
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nrp1^tm1Hfu/Nrp1^tm1Hfu	involves: C57BL/6 * CBA	MGI:3579403
hm2	Nrp1^tm1Hfu/Nrp1^tm1Hfu	involves: C57BL/6 * CBA * ICR	MGI:3036465
hm3	Nrp1^tm1Hfu/Nrp1^tm1Hfu	involves: C57BL/6NCrlj * CBA/JNCrlj	MGI:5432162
hm4	Nrp1^tm1Hfu/Nrp1^tm1Hfu	involves: CD-1 * JF1	MGI:3512162
cn5	Nrp1^tm1Hfu/Nrp1^tm2Ddg Tg(Tek-cre)1Ywa/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * SJL	MGI:5432163
cx6	Nrp1^tm1Hfu/Nrp1^+ Nrp2^tm1Mkl/Nrp2^tm1Mkl	involves: BALB/c * C57BL/6 * CBA	MGI:3712946
cx7	Nrp1^tm1Hfu/Nrp1^tm1Hfu Nrp2^tm1Mkl/Nrp2^+	involves: BALB/c * C57BL/6 * CBA	MGI:3712945

Genotype
MGI:3579403

hm1

• Allelic Composition: Nrp1^tm1Hfu/Nrp1^tm1Hfu
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:75477 )

• mice die at E12.5

cardiovascular system

abnormal vascular development ( J:75477 )

• defects as previously reported for mutations of this locus are observed

• however, mostly normal blood vessel network formation occurs in both yolk sac and embryo at E10

transposition of great arteries ( J:75477 )

nervous system

abnormal axon guidance ( J:98330 )

• growth cone collapse of dorsal root ganglion and sympathetic ganglion axons in response to Sema3A was completely abolished

abnormal trigeminal nerve morphology ( J:98330 )

• ophthalmic nerve fibers overshot their peripheral target fields

abnormal spinal nerve morphology ( J:98330 )

• spinal nerve fibers overshot toward the opposite side of embryos after crossing the dorsal midline

cellular

abnormal axon guidance ( J:98330 )

• growth cone collapse of dorsal root ganglion and sympathetic ganglion axons in response to Sema3A was completely abolished

Genotype
MGI:3036465

hm2

• Allelic Composition: Nrp1^tm1Hfu/Nrp1^tm1Hfu
• Genetic Background: involves: C57BL/6 * CBA * ICR

mortality/aging

embryonic lethality, complete penetrance ( J:58077 )

• by 13.5 dpc, most mutant embryos had died, but a few embryos with severe edema were still alive

cardiovascular system

abnormal dorsal aorta morphology ( J:58077 )

• at E10.5 segements of the dorsal aorta are regressed

abnormal pharyngeal arch artery morphology ( J:58077 )

• at E9.5 all embryos (N = 5) exhibited severe regression of the arch artery system (arch arteries 1 - 3 are normally bilaterally symmetrical at this time)

abnormal first pharyngeal arch artery morphology ( J:58077 )

• at E10.5 the arch arteries 1 and 2 often persisted

abnormal fourth pharyngeal arch artery morphology ( J:58077 )

• at E10.5, 15 out of 22 embryos lack the right and left arch arteries 4

• at E 12.5 the left arch artery 4, which normally forms the arch of the aorta is absent and the arch of the aorta forms on the right side (15 out of 21)

abnormal second pharyngeal arch artery morphology ( J:58077 )

• at E10.5 the arch arteries 1 and 2 often persisted

abnormal sixth pharyngeal arch artery morphology ( J:58077 )

• at E10.5, all embryos (N = 22) lack the right and left arch arteries 6

• at E12.5 all embryos (N = 21) lack the left arch artery 6

abnormal third pharyngeal arch artery morphology ( J:58077 )

• at E10.5, some embryos missing arch artery 4 also lack the arch artery 3 on either side

abnormal pulmonary artery morphology ( J:58077 )

• the distal end of the pulmonary channel merges with the aortic arch

abnormal aortic arch and aortic arch branch attachment ( J:58077 )

• at E12.5 the left subclavian artery arises from the right sided arch of the aorta

right aortic arch ( J:58077 )

• right arotic arch is observed in some mutants

double aortic arch ( J:58077 )

• observed in some mutants

decreased angiogenesis ( J:58077 )

• capillary invasion is absent from the central nervous system of mutants at E10.5

• at E12.5 little vascularization is seen in the neocortex, dorsal part of the midbrain, spinal cord, and sensory ganglia

• at E12.5 abnormal capillaries, that are of large caliber, with few branches and often broken into small spherical segments, are present in the diencephalon, ventral midbrain, hindbrain, and ventral spinal cord

disorganized yolk sac vascular plexus ( J:58077 )

• at E12.5 the yolk sac of mutants is as well vascularized as that of wild-types however the vascular networks are abnormal

• the large vessels meander and are often divided into small vessels that anastomose

• capillary networks in the yolk sac are sparse

persistent truncus arteriosus ( J:58077 )

• at E12.5 seperation of the truncus arteriosus is incomplete (5 out of 6)

embryo

abnormal pharyngeal arch artery morphology ( J:58077 )

• at E9.5 all embryos (N = 5) exhibited severe regression of the arch artery system (arch arteries 1 - 3 are normally bilaterally symmetrical at this time)

abnormal first pharyngeal arch artery morphology ( J:58077 )

• at E10.5 the arch arteries 1 and 2 often persisted

abnormal fourth pharyngeal arch artery morphology ( J:58077 )

• at E10.5, 15 out of 22 embryos lack the right and left arch arteries 4

• at E 12.5 the left arch artery 4, which normally forms the arch of the aorta is absent and the arch of the aorta forms on the right side (15 out of 21)

abnormal second pharyngeal arch artery morphology ( J:58077 )

• at E10.5 the arch arteries 1 and 2 often persisted

abnormal sixth pharyngeal arch artery morphology ( J:58077 )

• at E10.5, all embryos (N = 22) lack the right and left arch arteries 6

• at E12.5 all embryos (N = 21) lack the left arch artery 6

abnormal third pharyngeal arch artery morphology ( J:58077 )

• at E10.5, some embryos missing arch artery 4 also lack the arch artery 3 on either side

disorganized yolk sac vascular plexus ( J:58077 )

• at E12.5 the yolk sac of mutants is as well vascularized as that of wild-types however the vascular networks are abnormal

• the large vessels meander and are often divided into small vessels that anastomose

• capillary networks in the yolk sac are sparse

craniofacial

abnormal pharyngeal arch artery morphology ( J:58077 )

• at E9.5 all embryos (N = 5) exhibited severe regression of the arch artery system (arch arteries 1 - 3 are normally bilaterally symmetrical at this time)

abnormal first pharyngeal arch artery morphology ( J:58077 )

• at E10.5 the arch arteries 1 and 2 often persisted

abnormal fourth pharyngeal arch artery morphology ( J:58077 )

• at E10.5, 15 out of 22 embryos lack the right and left arch arteries 4

• at E 12.5 the left arch artery 4, which normally forms the arch of the aorta is absent and the arch of the aorta forms on the right side (15 out of 21)

abnormal second pharyngeal arch artery morphology ( J:58077 )

• at E10.5 the arch arteries 1 and 2 often persisted

abnormal sixth pharyngeal arch artery morphology ( J:58077 )

• at E10.5, all embryos (N = 22) lack the right and left arch arteries 6

• at E12.5 all embryos (N = 21) lack the left arch artery 6

abnormal third pharyngeal arch artery morphology ( J:58077 )

• at E10.5, some embryos missing arch artery 4 also lack the arch artery 3 on either side

Genotype
MGI:5432162

hm3

• Allelic Composition: Nrp1^tm1Hfu/Nrp1^tm1Hfu
• Genetic Background: involves: C57BL/6NCrlj * CBA/JNCrlj

nervous system

nervous system phenotype ( J:175836 )

N • normal numbers of gonadotropin-releasing hormone neurons

Genotype
MGI:3512162

hm4

• Allelic Composition: Nrp1^tm1Hfu/Nrp1^tm1Hfu
• Genetic Background: involves: CD-1 * JF1

nervous system

delayed neuronal migration ( J:94050 )

• delayed tangential somata migration of facial branchiomotor neurons that resulted in the separation of the migratory stream into several distinct streams on the ventricular side of the hindbrain

• facial branchiomotor neuron somata emerged on the pial side in an ectopic anterior location during radial migration

• migration defects are not due to abnormal hindbrain segmentation, vascular defects in the hindbrain or axon guidance defects in the periphery

abnormal facial motor nucleus morphology ( J:94050 )

• nuclei on the pial side were elongated or dumbbell-shaped and in severe cases, the entire nucleus was shifted anteriorly

abnormal facial nerve morphology ( J:94050 )

• defasciculated facial nerve

abnormal trigeminal nerve morphology ( J:94050 )

• defasciculated trigeminal nerve branches

cellular

delayed neuronal migration ( J:94050 )

• delayed tangential somata migration of facial branchiomotor neurons that resulted in the separation of the migratory stream into several distinct streams on the ventricular side of the hindbrain

• facial branchiomotor neuron somata emerged on the pial side in an ectopic anterior location during radial migration

• migration defects are not due to abnormal hindbrain segmentation, vascular defects in the hindbrain or axon guidance defects in the periphery

Genotype
MGI:5432163

cn5

• Allelic Composition: Nrp1^tm1Hfu/Nrp1^tm2Ddg; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * SJL

nervous system

abnormal brain vasculature morphology ( J:175836 )

• fewer and larger

decreased neuron number ( J:175836 )

• reduced gonadotropin-releasing hormone neurons

cardiovascular system

abnormal brain vasculature morphology ( J:175836 )

• fewer and larger

Genotype
MGI:3712946

cx6

• Allelic Composition: Nrp1^tm1Hfu/Nrp1⁺; Nrp2^tm1Mkl/Nrp2^tm1Mkl
• Genetic Background: involves: BALB/c * C57BL/6 * CBA

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:75477 )

• embryos die at E10-10.5, showing longer survival thah double homozygous embryos

embryo

abnormal vitelline vasculature morphology ( J:75477 )

• yolk sacs are of normal size, but lack larger collecting vessels and contain irregular and less dense capillary networks

• vasulature of yolk sac at E10 is characterized by vessel heterogeneity, disorganization, and immaturity

• thickened blood vessels, lack of capillaries, and large avascular spaces between blood vessels are observed, with occasional dead-ended sprouts not connected to other sprouts

• arterial and venous branching is not seen in yolk sacs at E10

cardiovascular system

abnormal vascular development ( J:75477 )

• embryos show severe vascular impairment at E10

• blood vessels are disorganized, heterogeneous in size, and some show thickening

• blood vessel density is low in the head region, with avascular regions in head and trunk

• small vessel sprouting is seen, but is unconnected to other sprouts and no capillary plexus is formed

abnormal dorsal aorta morphology ( J:75477 )

• dorsal aorta is poorly formed, and outflow tract is undetectable

abnormal vitelline vasculature morphology ( J:75477 )

• yolk sacs are of normal size, but lack larger collecting vessels and contain irregular and less dense capillary networks

• vasulature of yolk sac at E10 is characterized by vessel heterogeneity, disorganization, and immaturity

• thickened blood vessels, lack of capillaries, and large avascular spaces between blood vessels are observed, with occasional dead-ended sprouts not connected to other sprouts

• arterial and venous branching is not seen in yolk sacs at E10

hemorrhage ( J:75477 )

• multiple hemorrhages are observed in embryos at E10

Genotype
MGI:3712945

cx7

• Allelic Composition: Nrp1^tm1Hfu/Nrp1^tm1Hfu; Nrp2^tm1Mkl/Nrp2⁺
• Genetic Background: involves: BALB/c * C57BL/6 * CBA

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:75477 )

• embryos die at E10-10.5, showing longer survival thah double homozygous embryos

embryo

abnormal vitelline vasculature morphology ( J:75477 )

• yolk sacs are of normal size, but lack larger collecting vessels and contain irregular and less dense capillary networks

• vasulature of yolk sac at E10 is characterized by vessel heterogeneity, disorganization, and immaturity

• arterial and venous branching is not seen in yolk sacs at E10

• thickened blood vessels, lack of capillaries, and large avascular spaces between blood vessels are observed, with occasional dead-ended sprouts not connected to other sprouts

decreased embryo size ( J:75477 )

• much smaller than littermates; length of embryo in yolk sac is ~50% of control embryo length

growth/size/body

decreased embryo size ( J:75477 )

• much smaller than littermates; length of embryo in yolk sac is ~50% of control embryo length

cardiovascular system

abnormal vascular development ( J:75477 )

• avascular regions, vessel size heterogeneity, and thickened blood vessels are observed

• outflow tract formation is abnormal

• blood vessel density in head region is slightly higher than in Npr1^tm1Hfu/Npr1⁺; Npr2^tm1Mkl /Npr2^tm1Mkl embryos

abnormal dorsal aorta morphology ( J:75477 )

• abnormal formation of dorsal aorta is observed

abnormal vitelline vasculature morphology ( J:75477 )

• yolk sacs are of normal size, but lack larger collecting vessels and contain irregular and less dense capillary networks

• vasulature of yolk sac at E10 is characterized by vessel heterogeneity, disorganization, and immaturity

• arterial and venous branching is not seen in yolk sacs at E10

• thickened blood vessels, lack of capillaries, and large avascular spaces between blood vessels are observed, with occasional dead-ended sprouts not connected to other sprouts

hemorrhage ( J:75477 )

• multiple hemorrhages are observed in embryos at E10