Analysis Tools|
Allele Symbol Allele Name Allele ID |
Mdm2tm1Glo targeted mutation 1, Guillermina Lozano MGI:1857945 |
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| Summary |
30 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• hematopoietic stem and progenitor cells (HSPC) from irradiated mice transplanted into irradiated mice reconstitute the HSPC population exhibit a competitive disadvantage over HSPC from untreated mice compared with cells from similarly irradiated wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• homozygotes die between implantation and E6.5
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• at E5.5, mutant embryos have formed an active site of implantation but contain few, if any, cells relative to wild-type embryos
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• many empty decidua are observed between E6.5 and E10.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice treated with ionizing radiation exhibit increased mortality compared with similarly treated wild-type mice
• male mice are more sensitive to treatment with ionizing radiation than female mice
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• following transformation with c-myc and activated Ras oncogenes, mouse embryonic fibroblasts exhibit 33% fewer foci compared with wild-type cells
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• mice treated with ionizing radiation exhibit increased mortality compared with similarly treated wild-type mice
• male mice are more sensitive to treatment with ionizing radiation than female mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mild hyperpigmentation detectable beginning around 4 - 6 months of age
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• mild hyperpigmentation detectable beginning around 4 - 6 months of age
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• mild hyperpigmentation detectable beginning around 4 - 6 months of age
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• exhibit rescue of the heart lethality seen in conditional Mdm2 mice and have the same life span as single homozygous Trp53 mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• all die by E13.5, with abnormalities evident at E9.5 but not E9
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• smaller than controls, probably due to lack of blood flow, however they develop to the correct developmental stage
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• significant thinning of the myocardial layer in the ventricles
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• heart mass is smaller in E9.5 embryos and by E13.5, most homozygotes lack any visible signs of a heart
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• one of the E13.5 embryos that still has a heart shows abnormal trabeculation in the ventricle and abnormal ventricular wall structure
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• hearts fail to function properly as indicated by blood leaking outside the heart and the backup of blood in the atrium
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• decrease in blood flow that results in congestion and backup of blood in other organs
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• exhibit blood leaking outside the heart
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• significant thinning of the myocardial layer in the ventricles
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• smaller than controls, probably due to lack of blood flow, however they develop to the correct developmental stage
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• cell cycle arrest in irradiate MEFs is improved compared to Trp53 single homozygous MEFs but is still not as robust as in wild-type MEFs
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice exhibit normal mortality in response to treatment with ionizing radiation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• some die within 3 months
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• decreased compared to Trp53tm1.1Tldo/+ mice
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• decreased compared to Trp53tm1.1Tldo/+ mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• irradiation-induced cell cycle arrest is partially restored these MEFs compared to Trp53 single homozygous MEFs
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no double homozygous mice are found
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• compared with Mdm2tm1Glo heterozygotes
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• compared with Trp53tm2Wahl homozygotes
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• prior to and after irradiation compared with Trp53tm2Wahl homozygotes
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• prior to and after irradiation compared with Trp53tm2Wahl homozygotes
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• prior to and after irradiation compared with Trp53tm2Wahl homozygotes
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• severe in irradiated-mice
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• severe atrophic in irradiated-mice
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• compared with Trp53tm2Wahl homozygotes
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• prior to and after irradiation compared with Trp53tm2Wahl homozygotes
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• severe in irradiated-mice
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• compared with Mdm2tm1Glo heterozygotes
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• compared with Trp53tm2Wahl homozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• presence of one copy of Mdm2 partially restores the arrest response of Trp53tm1Wahl; p21 protein levels are increased slightly more compared to homozygous Trp53tm1Wahl, Mdm2tm1Glo/+ or homozygous Trp53tm1Wahl, Mdm4/+ heterozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no mice are present at weaning
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• fewer than expected mice are born
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• mice exhibit decreased extramedullary hematopoiesis in the liver
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• hypoplastic
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• disorganized
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• the granule layer of the cerebellum is hypoplastic with defects in both the external granule layer and the internal granule layer
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• 10% to 20% of mice exhibit exencephaly associated with cleft palate, or other types of neural tube closure defects, such as kinked tails
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• 10% to 20% of mice exhibit exencephaly associated with cleft palate, or other types of neural tube closure defects, such as kinked tails
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• mid-gestation
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• mice are smaller than normal at birth
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• following transformation with c-myc and activated Ras oncogenes, mouse embryonic fibroblasts exhibit fewer foci compared with wild-type or single heterozygote cells
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• in E13.5 embryos
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• fewer and smaller
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• 10% to 20% of mice exhibit exencephaly associated with cleft palate, or other types of neural tube closure defects, such as kinked tails
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• 10% to 20% of mice exhibit exencephaly associated with cleft palate, or other types of neural tube closure defects, such as kinked tails
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• in E13.5 embryos
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• mid-gestation
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• 10% to 20% of mice exhibit exencephaly associated with cleft palate, or other types of neural tube closure defects, such as kinked tails
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• bone marrow sections show hypocellularity indicative of aplastic anemia
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• distribution of Purkinje neurons is disorganized in the cerebellum
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no embryos are found at E8.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice are born in expected Mendelian ratios
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• bone marrow sections show hypocellularity indicative of aplastic anemia
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• distribution of Purkinje neurons is disorganized in the cerebellum
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• detectable first at P4
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• detectable first at P4
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• detectable first at P4
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• starting in early postnatal development
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• starting in early postnatal development
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• starting in early postnatal development
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• presence of one copy of Mdm2 partially restores the arrest response of Trp53tm1Wahl; p21 protein levels are modestly increased from homozygous Trp53 mutant levels
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no double homozygotes survive to birth
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• less mobile
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• as in Mdm2tm1Glo homozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• lethality observed in Mdm2tm1Glo homozygotes is completely rescued with mice surviving beyond 5 weeks
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/29/2024 MGI 6.24 |
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