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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Apex1tm1Djc
targeted mutation 1, David J Chen
MGI:1857955
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Apex1tm1Djc/Apex1tm1Djc involves: 129X1/SvJ * NTac:NIHS MGI:3834456
ht2
 		Apex1tm1Djc/Apex1+ involves: 129X1/SvJ * NTac:NIHS MGI:3834458
cx3
 		Apex1tm1Djc/Apex1+
Tg(TacLIZa)A1Jsh/0 		involves: 129X1/SvJ * C57BL/6 * NTac:NIHBS MGI:3834457


Genotype
MGI:3834456
hm1
Allelic
Composition		 Apex1tm1Djc/Apex1tm1Djc
Genetic
Background		 involves: 129X1/SvJ * NTac:NIHS
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apex1tm1Djc mutation (0 available); any Apex1 mutation (16 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:50702 )
• homozygous embryos die in utero; no embryos are recovered between days 10 to 13.5
• 25% of decidua are empty or necrotic between day 10.5 and 13.5; in litters harvested from day 7.5 to 9.5, only 15.5% of embryos recovered are homozygous versus expected 25%

embryo
abnormal embryo development ( J:50702 )
• at day 6.5, embryos have unusually high percentages of pyknotic nuclei
embryonic growth retardation ( J:50702 )
• in litters examined at day 8.5 and 9.5, embryos isolated from yolk sacs display marked growth retardation
abnormal embryonic epiblast morphology ( J:50702 )
• epiblast is abnormal at day 7.5
increased embryonic epiblast cell apoptosis ( J:50702 )
• day 6.5 epiblasts have high pyknotic indices compared to controls
abnormal visceral yolk sac morphology ( J:50702 )
• yolk sac persists to day 9.5

growth/size/body
embryonic growth retardation ( J:50702 )
• in litters examined at day 8.5 and 9.5, embryos isolated from yolk sacs display marked growth retardation

cellular
increased cellular sensitivity to gamma-irradiation ( J:50702 )
• explanted blastocysts exposed to different levels of gamma-irradiation show increased sensitivity to ionizing radiation relative to exposed heterozygous blastocysts cultures
increased embryonic epiblast cell apoptosis ( J:50702 )
• day 6.5 epiblasts have high pyknotic indices compared to controls




Genotype
MGI:3834458
ht2
Allelic
Composition		 Apex1tm1Djc/Apex1+
Genetic
Background		 involves: 129X1/SvJ * NTac:NIHS
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apex1tm1Djc mutation (0 available); any Apex1 mutation (16 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
abnormal seminiferous tubule morphology ( J:92957 )
• in 9-month-old mice numbers of tubules containing apoptotic cells is higher than in controls (32.8% vs 19.3%); proportion of apoptotic spermatogonia is also higher (54.4% vs 17.3%) at 9 months

liver/biliary system
abnormal liver morphology ( J:92957 )
• at 9 months, centrilobular fatty changes surrounding the central vein are common (in 50% of animals) compared to wild-type controls

reproductive system
abnormal seminiferous tubule morphology ( J:92957 )
• in 9-month-old mice numbers of tubules containing apoptotic cells is higher than in controls (32.8% vs 19.3%); proportion of apoptotic spermatogonia is also higher (54.4% vs 17.3%) at 9 months




Genotype
MGI:3834457
cx3
Allelic
Composition		 Apex1tm1Djc/Apex1+
Tg(TacLIZa)A1Jsh/0
Genetic
Background		 involves: 129X1/SvJ * C57BL/6 * NTac:NIHBS
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apex1tm1Djc mutation (0 available); any Apex1 mutation (16 available)
Tg(TacLIZa)A1Jsh mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal cell physiology ( J:92957 )
• spontaneous mutation frequency for mixed spermatogenic cells
abnormal DNA repair ( J:92957 )
• spontaneous mutation frequency for mixed spermatogenic cells from 9-month-old double mutant mice is 2-fold higher than for wild-type Apex1 hemizygous Tg mice
• spontaneous mutation frequency observed in liver samples in 3-month-old animals is significantly higher compared to age matched wild-type Apex1 hemizygous Tg mice; a further 1.5-fold increase is observed in samples from 9-month-old animals
• spontaneous mutation frequency observed in liver samples in 9-month-old animals is significantly higher compared to 3-month-old mice of the same genotype, suggesting an age-related increase in frequency
• spontaneous mutation frequency observed in spleen samples from 3-month-old mice is 2-fold higher than in controls and an additional 2-fold increase is found in samples from 9-month-old mice

homeostasis/metabolism
abnormal DNA repair ( J:92957 )
• spontaneous mutation frequency for mixed spermatogenic cells from 9-month-old double mutant mice is 2-fold higher than for wild-type Apex1 hemizygous Tg mice
• spontaneous mutation frequency observed in liver samples in 3-month-old animals is significantly higher compared to age matched wild-type Apex1 hemizygous Tg mice; a further 1.5-fold increase is observed in samples from 9-month-old animals
• spontaneous mutation frequency observed in liver samples in 9-month-old animals is significantly higher compared to 3-month-old mice of the same genotype, suggesting an age-related increase in frequency
• spontaneous mutation frequency observed in spleen samples from 3-month-old mice is 2-fold higher than in controls and an additional 2-fold increase is found in samples from 9-month-old mice




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory