Phenotypes associated with this allele

• Allele Symbol: Zp3^tm1Dean
• Allele Name: targeted mutation 1, Jurrien Dean
• Allele ID: MGI:1857971
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zp3^tm1Dean/Zp3^tm1Dean	involves: 129S4/SvJae * C57BL/6J	MGI:2660690
cx2	Tg(ZP2)2Dean/0 Tg(ZP3)7812Dean/? Zp2^tm1Dean/Zp2^tm1Dean Zp3^tm1Dean/Zp3^tm1Dean	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * CF-1 * FVB/N	MGI:3664455
cx3	Zp3^tm1Dean/Zp3^tm1Dean Tg(ZP3)7812Dean/?	involves: 129S4/SvJae * C57BL/6J * FVB/N	MGI:3663045

Genotype
MGI:2660690

hm1

• Allelic Composition: Zp3^tm1Dean/Zp3^tm1Dean
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

absent zona pellucida ( J:36772 )

♀ • oocytes lacked the zona pellucida matrix

endocrine/exocrine glands

abnormal ovarian folliculogenesis ( J:36772 )

♀ • disorganized corona radiata

♀ • abnormal cumulus-oocyte complex allowing oocyte to dissociate from the cumulus mass

reproductive system

absent zona pellucida ( J:36772 )

♀ • oocytes lacked the zona pellucida matrix

abnormal ovarian folliculogenesis ( J:36772 )

♀ • disorganized corona radiata

♀ • abnormal cumulus-oocyte complex allowing oocyte to dissociate from the cumulus mass

decreased superovulation rate ( J:36772 )

♀ • after hormone-induced ovulation, cumulus masses were present in the oviducts but zona-free eggs were observed in only half of the females and, in these, less than 10% of the normal number of eggs were recovered

♀ • recovered eggs were unfertilized

female infertility ( J:36772 )

♀

Genotype
MGI:3664455

cx2

• Allelic Composition: Tg(ZP2)2Dean/0; Tg(ZP3)7812Dean/?; Zp2^tm1Dean/Zp2^tm1Dean; Zp3^tm1Dean/Zp3^tm1Dean
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * CF-1 * FVB/N

reproductive system

reproductive system phenotype ( J:93482 )

N • ovarian histology of mice expressing human ZP2 and ZP3 in place of the native mouse proteins appears normal

N • zonae pellucidae of oocytes from mice expressing human ZP2 and ZP3 in place of their mouse orthologs appear similar histologically to those of wild-type mice; human ZP2 and ZP3 proteins are immunohistologically detectable, with mouse ZP1, throughout the zona matrix

N • cumulus-free ovulated oocytes expressing human instead of mouse ZP2 and ZP3 bind mouse sperm, but fail to bind human sperm

reduced female fertility ( J:93482 )

♀ • female mice expressing human in place of mouse ZP2 and ZP3 ovulate approximately half the number of oocytes as do wild-type females when primed with pregnant mare serum and stimulated with human chorionic gonadotrophin

decreased litter size ( J:93482 )

• although human ZP2/ZP3 transgenes rescue the sterility of female mice lacking mouse ZP2 and ZP3, their average litter size is smaller than that of wild-type or ZP2 rescue mice

embryo

abnormal embryo development ( J:93482 )

• one- and two-cell embryos having human in place of mouse ZP2 and ZP3 exhibit persistent sperm binding, even after multiple washes using a wide-bore pipet

• cleavage of mouse ZP2 following fertilization is demonstrated by increased SDS-PAGE mobility of the reduced protein in the wild-type two-cell embryo versus in the ovulated oocyte; this mobility shift fails to occur in two-cell embryos in which human ZP2 and ZP3 replace their mouse orthologs

failure of zygotic cell division ( J:93482 )

• 24 hours after in vitro insemination, only 41% of ovulated oocytes in cumulus mass from female mice expressing human in place of mouse ZP2 and ZP3 had progressed to the 2-cell stage, versus 82% of wild-type and 68% of ZP2 rescue eggs

Genotype
MGI:3663045

cx3

• Allelic Composition: Zp3^tm1Dean/Zp3^tm1Dean; Tg(ZP3)7812Dean/?
• Genetic Background: involves: 129S4/SvJae * C57BL/6J * FVB/N

cellular

thin zona pellucida ( J:93482 )

♀ • the failure of zona pellucida formation by oocytes of mice lacking mouse ZP3 is rescued by expression of human ZP3, although these zonae appear thinner than those of wild-type oocytes or those expressing both mouse and human ZP3; the rescued zonae contain mouse ZP1 and ZP2

reproductive system

reproductive system phenotype ( J:77459 )

N • whereas female mice lacking ZP3 are infertile, those expressing human instead of mouse ZP3 produce normal offspring; in early generations their litter sizes were smaller than those of wild-type females or those expressing both mouse and human ZP3, but later studies have not borne out this observation

N • whereas few or no eggs are recovered from gonadotropin-stimulated female mice lacking ZP3, similar numbers of eggs are retrieved from wild-type and human ZP3 rescue mice

N • mouse sperm bind in vitro to cumulus-free ovulated oocytes in which mouse ZP3 has been replaced by human ZP3, but human sperm do not attach even transiently

thin zona pellucida ( J:93482 )

♀ • the failure of zona pellucida formation by oocytes of mice lacking mouse ZP3 is rescued by expression of human ZP3, although these zonae appear thinner than those of wild-type oocytes or those expressing both mouse and human ZP3; the rescued zonae contain mouse ZP1 and ZP2

embryo

embryo phenotype ( J:93482 )

N • one- and two-celled embryos having the human protein in place of mouse ZP3, like wild-type embryos, do not continue to bind mouse sperm