Phenotypes associated with this allele

• Allele Symbol: Grik2^tm1Sfh
• Allele Name: targeted mutation 1, Stephen F Heinemann
• Allele ID: MGI:1857979
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Grik2^tm1Sfh/Grik2^tm1Sfh	involves: 129S1/Sv	MGI:3844283
hm2	Grik2^tm1Sfh/Grik2^tm1Sfh	involves: 129S1/Sv * C57BL/6	MGI:2176814
cx3	Grik1^tm1Sfh/Grik1^tm1Sfh Grik2^tm1Sfh/Grik2^tm1Sfh	involves: 129S/SvEv * 129S1/Sv * C57BL/6	MGI:3639308

Genotype
MGI:3844283

hm1

• Allelic Composition: Grik2^tm1Sfh/Grik2^tm1Sfh
• Genetic Background: involves: 129S1/Sv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal CNS synaptic transmission ( J:148310 )

• low frequency facilitation is reduced at a stimulation rate of 1 Hz in mossy fiber CA3 synapses

• addition of philanthotoxin-433 fails to reduce low frequency stimulation unlike in wild-type mice

decreased paired-pulse facilitation ( J:148310 )

• smaller mossy fiber paired pulse facilitation at short interstimulus intervals

Genotype
MGI:2176814

hm2

• Allelic Composition: Grik2^tm1Sfh/Grik2^tm1Sfh
• Genetic Background: involves: 129S1/Sv * C57BL/6

growth/size/body

decreased body weight ( J:46932 )

nervous system

decreased susceptibility to pharmacologically induced seizures ( J:46932 )

• almost all homozygotes are protected against kainate-induced seizures after intraperitoneal injection of 20 mg kainate/kg

• however; performance in other behavioral tests including rotarod, Morris water maze, and sensorimotor tests is similar to wild-type

abnormal glutamate-mediated receptor currents ( J:46932 )

• kainate fails to activate inward currents in CA3 neurons of the hippocampus at concentrations up to 3uM

abnormal long-term potentiation ( J:91763 )

• LTP induced significantly less than controls in hippocampal mossy fibers

reduced long-term potentiation ( J:98109 )

• theta burst stimulation fails to produce long term potentiation in the lateral amygdala

decreased paired-pulse facilitation ( J:91763 )

behavior/neurological

decreased susceptibility to pharmacologically induced seizures ( J:46932 )

• almost all homozygotes are protected against kainate-induced seizures after intraperitoneal injection of 20 mg kainate/kg

• however; performance in other behavioral tests including rotarod, Morris water maze, and sensorimotor tests is similar to wild-type

abnormal contextual conditioning behavior ( J:98109 )

• reduced contextual fear memory

abnormal cued conditioning behavior ( J:98109 )

• reduced auditory fear memory

decreased fear-related response ( J:98109 )

• reduced contextual and auditory fear memory

Genotype
MGI:3639308

cx3

• Allelic Composition: Grik1^tm1Sfh/Grik1^tm1Sfh; Grik2^tm1Sfh/Grik2^tm1Sfh
• Genetic Background: involves: 129S/SvEv * 129S1/Sv * C57BL/6

nervous system

abnormal inhibitory postsynaptic currents ( J:66021 )

• spontaneous IPSC frequency increases after domoate stimulation is completely eliminated in CA1 pyramidal neurons

• evoked IPSC is eliminated