Phenotypes associated with this allele

• Allele Symbol: Pik3cg^tm1Wym
• Allele Name: targeted mutation 1, Matthias P Wymann
• Allele ID: MGI:1858012
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pik3cg^tm1Wym/Pik3cg^tm1Wym	either: 129/Sv-Pik3cg^tm1Wym or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)	MGI:3619268
hm2	Pik3cg^tm1Wym/Pik3cg^tm1Wym	involves: 129S1/Sv * 129X1/SvJ	MGI:3619935

Genotype
MGI:3619268

hm1

• Allelic Composition: Pik3cg^tm1Wym/Pik3cg^tm1Wym
• Genetic Background: either: 129/Sv-Pik3cg^tm1Wym or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased neutrophil cell number ( J:60349 )

• exhibit about twice as many circulating polymorphonuclear neutrophils (PMNs) as wild-type

abnormal leukocyte physiology ( J:60349 )

• peritoneal leukocytes show a 36% decrease in accumulation in an aseptic peritonitis model

impaired macrophage chemotaxis ( J:60349 )

• peritoneal macrophages show reduced chemotaxis toward various chemoattractants (endotoxin-activated mouse serum, RANTES, MIP-5, MDC, SDF-1, and C5a, VEGF)

abnormal neutrophil physiology ( J:60349 )

• neutrophils display impaired resipiratory burst and motility

impaired neutrophil chemotaxis ( J:60349 )

• polymorphonuclear neutrophils show reduced migration toward a wide range of chemotactic stimuli (IL-8, fMLP, C5a)

impaired neutrophil recruitment ( J:60349 )

• 60% fewer polymorphonuclear neutrophils infiltrate subcutaneous air pouches after IL-8 administration

increased susceptibility to bacterial infection ( J:60349 )

• mutants are unable to clear peritoneal bacteria after administration of sublethal doses of S. aureus, with bacteria concentrations in the abdominal cavity 10x that of wild-type

hematopoietic system

increased neutrophil cell number ( J:60349 )

• exhibit about twice as many circulating polymorphonuclear neutrophils (PMNs) as wild-type

abnormal leukocyte physiology ( J:60349 )

• peritoneal leukocytes show a 36% decrease in accumulation in an aseptic peritonitis model

impaired macrophage chemotaxis ( J:60349 )

• peritoneal macrophages show reduced chemotaxis toward various chemoattractants (endotoxin-activated mouse serum, RANTES, MIP-5, MDC, SDF-1, and C5a, VEGF)

abnormal neutrophil physiology ( J:60349 )

• neutrophils display impaired resipiratory burst and motility

impaired neutrophil chemotaxis ( J:60349 )

• polymorphonuclear neutrophils show reduced migration toward a wide range of chemotactic stimuli (IL-8, fMLP, C5a)

impaired neutrophil recruitment ( J:60349 )

• 60% fewer polymorphonuclear neutrophils infiltrate subcutaneous air pouches after IL-8 administration

cellular

impaired macrophage chemotaxis ( J:60349 )

• peritoneal macrophages show reduced chemotaxis toward various chemoattractants (endotoxin-activated mouse serum, RANTES, MIP-5, MDC, SDF-1, and C5a, VEGF)

impaired neutrophil chemotaxis ( J:60349 )

• polymorphonuclear neutrophils show reduced migration toward a wide range of chemotactic stimuli (IL-8, fMLP, C5a)

Genotype
MGI:3619935

hm2

• Allelic Composition: Pik3cg^tm1Wym/Pik3cg^tm1Wym
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

immune system

abnormal neutrophil physiology ( J:147259 )

• reduced ability to produce reactive oxygen species (ROS)

impaired neutrophil chemotaxis ( J:238900 )

impaired neutrophil recruitment ( J:147259 )

• reduced recruitment to the peritoneum in thioglycollate induced peritonitis

cardiovascular system

increased cardiac muscle contractility ( J:79151 )

• exhibit increased fractional shortening, indicating enhanced contractility, however exhibit no evidence of hypertrophy or myocardial fibrosis

cellular

impaired neutrophil chemotaxis ( J:238900 )

muscle

increased cardiac muscle contractility ( J:79151 )

• exhibit increased fractional shortening, indicating enhanced contractility, however exhibit no evidence of hypertrophy or myocardial fibrosis

hematopoietic system

abnormal neutrophil physiology ( J:147259 )

• reduced ability to produce reactive oxygen species (ROS)

impaired neutrophil chemotaxis ( J:238900 )

impaired neutrophil recruitment ( J:147259 )

• reduced recruitment to the peritoneum in thioglycollate induced peritonitis