Phenotypes associated with this allele

• Allele Symbol: Met^tm1Cpo
• Allele Name: targeted mutation 1, Carola Ponzetto
• Allele ID: MGI:1858019
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Met^tm1Cpo/Met^tm1Cpo	involves: 129S1/Sv * 129X1/SvJ	MGI:5285659
hm2	Met^tm1Cpo/Met^tm1Cpo	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3773054
hm3	Met^tm1Cpo/Met^tm1Cpo	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:2450979
ht4	Met^tm1Cpo/Met^tm2Cpo	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:2450977
cn5	Met^tm1Cpo/Met^tm1Sst Tg(Nes-cre)1Kln/?	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL	MGI:5052134
cn6	Met^tm1Cpo/Met^tm1Sst Tg(Nes-cre)1Kln/0	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:5285657
cx7	Met^tm1Cpo/Met^tm1Cpo Pax3^tm2.1(PAX3/FOXO1A)Buck/Pax3^+	involves: 129 * BALB/c * C57BL/6	MGI:3690115
cx8	Met^tm1Cpo/Met^+ Pax3^tm2.1(PAX3/FOXO1A)Buck/Pax3^+	involves: 129 * BALB/c * C57BL/6	MGI:3690114

Genotype
MGI:5285659

hm1

• Allelic Composition: Met^tm1Cpo/Met^tm1Cpo
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:174591 )

N • mice exhibit normal numbers of motor neurons in the spinal cord

abnormal axon extension ( J:174591 )

• cutaneous maximus nerves exhibit pronounced axonal growth defects compared with wild-type nerves

abnormal neuron physiology ( J:174591 )

• cultured motor neurons fail to exhibit an effect on neuron survival in response to HGF treatment compared with similarly treated wild-type mice

cellular

abnormal axon extension ( J:174591 )

• cutaneous maximus nerves exhibit pronounced axonal growth defects compared with wild-type nerves

Genotype
MGI:3773054

hm2

• Allelic Composition: Met^tm1Cpo/Met^tm1Cpo
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

muscle

muscle phenotype ( J:98439 )

N • abdominal muscles and tail muscles are normal

absent skeletal muscle ( J:98439 )

• perineal muscles are absent at E15 in all embryos examined, with only 2 muscles in pelvic region present; all hindlimb muscles are absent

Genotype
MGI:2450979

hm3

• Allelic Composition: Met^tm1Cpo/Met^tm1Cpo
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:36244 )

• embryonic death starts at E13.5

• no live embryos beyond E17.5

embryo

decreased embryo size ( J:36244 )

small placenta ( J:36244 )

pale placenta ( J:36244 )

growth/size/body

abnormal tongue muscle morphology ( J:36244 )

• tip of tongue lacked muscle

decreased embryo size ( J:36244 )

muscle

abnormal tongue muscle morphology ( J:36244 )

• tip of tongue lacked muscle

abnormal muscle development ( J:36244 )

• myotubes completely absent in forelimbs

absent diaphragm ( J:36244 )

• absent

craniofacial

abnormal tongue muscle morphology ( J:36244 )

• tip of tongue lacked muscle

digestive/alimentary system

abnormal tongue muscle morphology ( J:36244 )

• tip of tongue lacked muscle

Genotype
MGI:2450977

ht4

• Allelic Composition: Met^tm1Cpo/Met^tm2Cpo
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

neonatal lethality, complete penetrance ( J:36244 )

• animals die within a few minutes after birth due to respiratory failure

• animals display curled fetal position

muscle

decreased skeletal muscle mass ( J:36244 )

• reduced total muscle mass

respiratory system

respiratory failure ( J:36244 )

Genotype
MGI:5052134

cn5

• Allelic Composition: Met^tm1Cpo/Met^tm1Sst; Tg(Nes-cre)1Kln/?
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

vision/eye

vision/eye phenotype ( J:173661 )

N • no effect on retinal ganglion cell survival after optic nerve axotomy

Genotype
MGI:5285657

cn6

• Allelic Composition: Met^tm1Cpo/Met^tm1Sst; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

nervous system

abnormal motor neuron innervation pattern ( J:174591 )

• mice exhibit reduced motor axon arborization (length and number of branches) in the pectoralis minor muscle compared with wild-type mice

• at P2, endplates in the pectoralis minor exhibit increased partially or completely denervated synapses compared with wild-type endplates

• however, mice exhibit normal innervation of the pectoralis minor muscle at E15.5 and of the pectoralis major

decreased motor neuron number ( J:174591 )

• by P2 in the C8 and T1 region of the spinal cord with no further reduction in adult mice

abnormal neuromuscular synapse morphology ( J:174591 )

• mice exhibit reduced motor axon arborization (length and number of branches) in the pectoralis minor muscle compared with wild-type mice

• at P2, endplates in the pectoralis minor exhibit increased partially or completely denervated synapses compared with wild-type endplates

• however, mice exhibit normal innervation of the pectoralis minor muscle at E15.5 and of the pectoralis major

behavior/neurological

impaired coordination ( J:174591 )

• in a wire hang test but not a rotarod

Genotype
MGI:3690115

cx7

• Allelic Composition: Met^tm1Cpo/Met^tm1Cpo; Pax3^{tm2.1(PAX3/FOXO1A)Buck}/Pax3⁺
• Genetic Background: involves: 129 * BALB/c * C57BL/6

embryo

embryo phenotype ( J:86911 )

N • complete rescue of the Pax3^{tm2.1(PAX3/FOXO1A)Buck} single heterozygote somite phenotype

Genotype
MGI:3690114

cx8

• Allelic Composition: Met^tm1Cpo/Met⁺; Pax3^{tm2.1(PAX3/FOXO1A)Buck}/Pax3⁺
• Genetic Background: involves: 129 * BALB/c * C57BL/6

embryo

embryo phenotype ( J:86911 )

N • ectopic somite delamination is not seen, unlike in Pax3^{tm2.1(PAX3/FOXO1A)Buck} single heterozygotes