Phenotypes associated with this allele

• Allele Symbol: Vasp^tm1Mzim
• Allele Name: targeted mutation 1, Michael Zimmer
• Allele ID: MGI:1858032
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vasp^tm1Mzim/Vasp^tm1Mzim	involves: 129P2/OlaHsd * C57BL/6	MGI:2653842

Genotype
MGI:2653842

hm1

• Allelic Composition: Vasp^tm1Mzim/Vasp^tm1Mzim
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:56356 )

N • homozygotes show no differences in platelet number in total blood and platelet-rich plasma relative to wild-type littermates

increased megakaryocyte cell number ( J:56356 )

• homozygotes display a moderate megakaryocyte hyperplasia in bone marrow and spleen

abnormal platelet activation ( J:56356 )

• upon stimulation of platelet-rich plasma with 0.5 units/ml of thrombin, mutant platelets show a 2-fold increase in surface expression of P-selectin and fibrinogen binding relative to wild-type platelets, indicating enhanced agonist-induced platelet activation

• unexpectedly, preincubation with the cAMP-elevating prostaglandin E1 completely abolished thrombin-induced fibrinogen binding and P-selectin expression, as well as platelet aggregation, in both wild-type and homozygous mutant platelets

homeostasis/metabolism

abnormal platelet activation ( J:56356 )

• upon stimulation of platelet-rich plasma with 0.5 units/ml of thrombin, mutant platelets show a 2-fold increase in surface expression of P-selectin and fibrinogen binding relative to wild-type platelets, indicating enhanced agonist-induced platelet activation

• unexpectedly, preincubation with the cAMP-elevating prostaglandin E1 completely abolished thrombin-induced fibrinogen binding and P-selectin expression, as well as platelet aggregation, in both wild-type and homozygous mutant platelets