Analysis Tools
mortality/aging
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• embryos die prior to E8.5
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embryo
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• at E7.5, embryos are severely developmentally retarded
• in cultured blastocysts, impaired outgrowth of the inner cell mass is seen after 5 days in culture
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• at E4.5-E5.5, embryos are at least 50% smaller than controls
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• at E7.5, no mesoderm formation is apparent
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• by E6.5, embryos show cellular disorganization and are starting to degenerate
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growth/size/body
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• at E7.5, embryos are severely developmentally retarded
• in cultured blastocysts, impaired outgrowth of the inner cell mass is seen after 5 days in culture
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• at E4.5-E5.5, embryos are at least 50% smaller than controls
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cellular
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• in vitro experiments show that the reduced size of mutant embryos is due to a decrease in embryonic cell proliferation and is not due to apoptosis
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