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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Slc30a3tm1Rpa
targeted mutation 1, Richard D Palmiter
MGI:1859820
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Slc30a3tm1Rpa/Slc30a3tm1Rpa involves: 129S7/SvEvBrd * C57BL/6 MGI:3029270
hm2
Slc30a3tm1Rpa/Slc30a3tm1Rpa involves: 129S7/SvEvBrd * C57BL/6 * SJL MGI:4366953
ht3
Slc30a3tm1Rpa/Slc30a3+ involves: 129S7/SvEvBrd * C57BL/6 MGI:3848800
cx4
Slc30a3tm1Rpa/Slc30a3tm1Rpa
Tg(APPSWE)2576Kha/0
involves: 129S7/SvEvBrd * C57BL/6 * SJL MGI:3029279


Genotype
MGI:3029270
hm1
Allelic
Composition
Slc30a3tm1Rpa/Slc30a3tm1Rpa
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc30a3tm1Rpa mutation (1 available); any Slc30a3 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• homozygotes display a ~20% reduction in total zinc levels in the hippocampus and cortex relative to wild-type mice
• Timm stain (a stain for histochemically reactive zinc) is undetectable in the hippocampus, neocortex, piriform cortex, amygdala, entorhinal cortex, striatum, olfactory bulb, and cochlear nucleus of homozygous mutant mice
• in the hippocampus, Timm staining is undetectable in the mossy fibers projecting from dentate granule neurons to the hilus and s lucidum and s oriens of the CA3 region, and in projections to s radiatum and s oriens of the CA1 region
• no Timm-staining is observed within synaptic vesicles of homozygous mutant mossy fiber boutons
• in contrast, Timm staining is readily detectable in the choroid plexus and in convoluted tubule cells of the submaxillary gland, while TSQ fluorescence is still abundant in differentiating spermatids and in beta-islet cells of the pancreas
• TSQ fluorescence (a specific indicator of vesicular zinc) is undetectable in the hilus and CA3 and CA1 regions of mutant hippocampus
• mutant hippocampi display normal mossy fiber bouton ultrastructure, with densely packed clear, round synaptic vesicles, a few dense core vesicles, and numerous mitochondria, but lack histochemically reactive zinc within the synaptic vesicles
• no Timm-staining is observed within synaptic vesicles of homozygous mutant mossy fiber boutons, indicating that histochemically reactive zinc is eliminated from synaptic vesicles

homeostasis/metabolism
• homozygotes exhibit impaired zinc transport into synaptic vesicles
• homozygotes display a ~20% reduction in total zinc levels in the hippocampus and cortex relative to wild-type mice
• Timm stain (a stain for histochemically reactive zinc) is undetectable in the hippocampus, neocortex, piriform cortex, amygdala, entorhinal cortex, striatum, olfactory bulb, and cochlear nucleus of homozygous mutant mice
• in the hippocampus, Timm staining is undetectable in the mossy fibers projecting from dentate granule neurons to the hilus and s lucidum and s oriens of the CA3 region, and in projections to s radiatum and s oriens of the CA1 region
• no Timm-staining is observed within synaptic vesicles of homozygous mutant mossy fiber boutons
• in contrast, Timm staining is readily detectable in the choroid plexus and in convoluted tubule cells of the submaxillary gland, while TSQ fluorescence is still abundant in differentiating spermatids and in beta-islet cells of the pancreas
• TSQ fluorescence (a specific indicator of vesicular zinc) is undetectable in the hilus and CA3 and CA1 regions of mutant hippocampus




Genotype
MGI:4366953
hm2
Allelic
Composition
Slc30a3tm1Rpa/Slc30a3tm1Rpa
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc30a3tm1Rpa mutation (1 available); any Slc30a3 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• zinc is not observed in the hippocampus and cerebral cortex, even in the normally zinc-rich mossy fibers
• total zinc content of cerebrum is reduced by about 30%

nervous system
• zinc is not observed in the hippocampus and cerebral cortex, even in the normally zinc-rich mossy fibers
• total zinc content of cerebrum is reduced by about 30%




Genotype
MGI:3848800
ht3
Allelic
Composition
Slc30a3tm1Rpa/Slc30a3+
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc30a3tm1Rpa mutation (1 available); any Slc30a3 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• heterozygotes display a ~10% reduction in total zinc levels in the hippocampus and cortex relative to wild-type mice
• Timm stain is reduced in the hippocampus, neocortex, piriform cortex, amygdala, entorhinal cortex, striatum, olfactory bulb, and cochlear nucleus of heterozygous mutant mice
• in the hippocampus, Timm staining is reduced in the mossy fibers projecting from dentate granule neurons to the hilus and s lucidum and s oriens of the CA3 region, and in projections to s radiatum and s oriens of the CA1 region
• Timm-staining is reduced within the synaptic vesicles of heterozygous mutant mossy fiber boutons
• TSQ fluorescenceis reduced by 47% in the hilus, 39% in the CA3 region, and 50% in the CA1 region of heterozygous mutant hippocampi

nervous system
• heterozygotes display a ~10% reduction in total zinc levels in the hippocampus and cortex relative to wild-type mice
• Timm stain is reduced in the hippocampus, neocortex, piriform cortex, amygdala, entorhinal cortex, striatum, olfactory bulb, and cochlear nucleus of heterozygous mutant mice
• in the hippocampus, Timm staining is reduced in the mossy fibers projecting from dentate granule neurons to the hilus and s lucidum and s oriens of the CA3 region, and in projections to s radiatum and s oriens of the CA1 region
• Timm-staining is reduced within the synaptic vesicles of heterozygous mutant mossy fiber boutons
• TSQ fluorescenceis reduced by 47% in the hilus, 39% in the CA3 region, and 50% in the CA1 region of heterozygous mutant hippocampi




Genotype
MGI:3029279
cx4
Allelic
Composition
Slc30a3tm1Rpa/Slc30a3tm1Rpa
Tg(APPSWE)2576Kha/0
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc30a3tm1Rpa mutation (1 available); any Slc30a3 mutation (28 available)
Tg(APPSWE)2576Kha mutation (5 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• plaque formation and insoluble amyloid beta levels are reduced relative to hemizygous Tg(APPSWE)2576HKahs mice that carried two wild copies of Slc30a3
• no sexual difference in synaptic zinc levels, plaque formation, or insoluble amyloid beta levels
• plaques are smaller in size than in single Tg(APPSWE)2576Kha hemizygotes
• mutants exhibit an increased ratio of souluble/insoluble amyloid beta at 18 months of age compared to single Tg(APPSWE)2576Kha hemizygotes
• males and females exhibit a decrease in levels of synaptic and total zinc compared to single Tg(APPSWE)2576Kha hemizygotes

homeostasis/metabolism
• plaque formation and insoluble amyloid beta levels are reduced relative to hemizygous Tg(APPSWE)2576HKahs mice that carried two wild copies of Slc30a3
• no sexual difference in synaptic zinc levels, plaque formation, or insoluble amyloid beta levels
• plaques are smaller in size than in single Tg(APPSWE)2576Kha hemizygotes
• mutants exhibit an increased ratio of souluble/insoluble amyloid beta at 18 months of age compared to single Tg(APPSWE)2576Kha hemizygotes
• males and females exhibit a decrease in levels of synaptic and total zinc compared to single Tg(APPSWE)2576Kha hemizygotes





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory