Phenotypes associated with this allele

• Allele Symbol: Tnfrsf8^tm1Mak
• Allele Name: targeted mutation 1, Tak Mak
• Allele ID: MGI:1860056
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tnfrsf8^tm1Mak/Tnfrsf8^tm1Mak	involves: 129P2/OlaHsd * C57BL/6	MGI:3036178
hm2	Tnfrsf8^tm1Mak/Tnfrsf8^tm1Mak	involves: C57BL/6	MGI:3055174

Genotype
MGI:3036178

hm1

• Allelic Composition: Tnfrsf8^tm1Mak/Tnfrsf8^tm1Mak
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

enlarged thymus ( J:62854 )

increased thymocyte number ( J:62854 )

• double-positive thymocytes less susceptible to anti-CD3-mediated apoptosis

immune system

immune system phenotype ( J:62854 )

N • normal absolute numbers of cells in spleen, lymph node, and bone marrow

N • no developmental abnormalities in T or B cell lineages

N • no overt histological changes in spleen and lymph nodes

N • normal T cell proliferation in response to anti-CD3 Ab, phorbol myristate acetate-calcium ionophore, and staphylococcal enterotoxin B

N • normal apoptotic T cell death in in vitro assays activation assays or after in vivo stimulation with staphylococcal enterotoxin B

N • normal CD8+ cytotoxic T cell memory in vitro and in vivo

N • normal basal serum levels of IgM, IgG1, IgG2a, IgG2b, IgG3

N • normal humoral immune response, both T-dependent and T-independent

enlarged thymus ( J:62854 )

increased thymocyte number ( J:62854 )

• double-positive thymocytes less susceptible to anti-CD3-mediated apoptosis

endocrine/exocrine glands

enlarged thymus ( J:62854 )

increased thymocyte number ( J:62854 )

• double-positive thymocytes less susceptible to anti-CD3-mediated apoptosis

Genotype
MGI:3055174

hm2

• Allelic Composition: Tnfrsf8^tm1Mak/Tnfrsf8^tm1Mak
• Genetic Background: involves: C57BL/6

immune system

decreased susceptibility to graft versus host disease ( J:92723 )

• increased host survival is seen after transplant of 0.3x10⁵ mutant CD4⁺ T cells into sublethally irradiated bm12 recipients compared to transplant of wild-type T cells

• no difference in survival is seen at higher CD4⁺ T cell concentrations or when CD8⁺ T cells are used