All Phenotypes Tnf<tm1Ljo> MGI Mouse

About Help FAQ

Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tnf^tm1Ljo
targeted mutation 1, Lloyd J Old
MGI:1860250

Summary

12 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tnf^tm1Ljo/Tnf^tm1Ljo	B6.129S1-Tnf^tm1Ljo	MGI:3802476
hm2	Tnf^tm1Ljo/Tnf^tm1Ljo	either: B6.129-Tnf^tm1Ljo or (involves: 129 * C57BL/6)	MGI:3582278
hm3	Tnf^tm1Ljo/Tnf^tm1Ljo	involves: 129S1/Sv	MGI:2175017
hm4	Tnf^tm1Ljo/Tnf^tm1Ljo	involves: 129S1/Sv * C57BL/6	MGI:3802520
hm5	Tnf^tm1Ljo/Tnf^tm1Ljo	involves: 129S1/Sv * C57BL/6J	MGI:4867650
ht6	Tnf^tm1Ljo/Tnf⁺	involves: 129S1/Sv	MGI:2175018
cx7	Nfkb1^tm1Bal/Nfkb1^tm1Bal Rela^tm1Bal/Rela^tm1Bal Tnf^tm1Ljo/Tnf^tm1Ljo	involves: 129P2/OlaHsd * 129S1/Sv * 129S4/SvJae	MGI:3046863
cx8	Rel^tm1Grd/Rel^tm1Grd Rela^tm1Bal/Rela^tm1Bal Tnf^tm1Ljo/Tnf^tm1Ljo	involves: 129P2/OlaHsd * 129S1/Sv * 129S4/SvJae	MGI:3046865
cx9	Rela^tm1Bal/Rela^tm1Bal Tnf^tm1Ljo/Tnf^tm1Ljo	involves: 129S1/Sv * 129S4/SvJae * C57BL/6	MGI:3799163
cx10	Tnf^tm1Ljo/Tnf^tm1Ljo Tnip1^tm1.1Ama/Tnip1^tm1.1Ama	involves: 129S1/Sv * C57BL/6J	MGI:3835806
cx11	Tnf^tm1Ljo/Tnf⁺ Tnip1^tm1.1Ama/Tnip1^tm1.1Ama	involves: 129S1/Sv * C57BL/6J	MGI:3835807
cx12	Rela^tm1Yuo/Rela^tm1Yuo Tnf^tm1Ljo/Tnf^tm1Ljo	involves: 129S/SvEv * 129S1/Sv	MGI:3799266

Allelic Composition	Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	B6.129S1-Tnf^tm1Ljo

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

abnormal long-term spatial reference memory ( J:120489 )

• one week following surgery to produce cortical controlled impact (CCI) brain injury, 6-9 week-old null mice show significantly less severe memory deficits than brain-injured wild-type controls based on performance scores in the Morris water maze

abnormal motor coordination/balance ( J:120489 )

• at 48 hours after CCI, both injured mutants and controls show significant motor deficits (neuroscores), but those in mutants are less severe than in injured wild-type mice

• at 1 week post-injury, mutants and wild-type mice with CCI display similar neuroscores, and by 3 weeks, wild-type mice show recovery of motor function to wild-type levels whereas Tnf-null injured mice do not display any significant recovery with time

impaired coordination ( J:120489 )

• brain-injured Tnf-null mice perform better than injured wild-type in balance beam and rotarod tests at 48 hours post-injury; by 3 weeks, wild-type mice show significant recovery to uninjured control levels while injured mutants display little recovery of function

nervous system

abnormal cerebral cortex morphology ( J:120489 )

• by 1 week post-injury, brain-injured wild-type and Tnf-null mice display a well-developed cavity in ipsilateral cortex; at 4 weeks, in injured wild-type mice, cavity does not extend beyond cortex, but in mutants, cavity reaches white matter or may even become confluent with ventricle

• at 1 week, cavity volume is comparable between wild-type and null mice, but by 2 weeks post-injury, mutants show a greater cortical injury cavity volume than wild-type

cellular

decreased apoptosis ( J:127028 )

• after bleomycin injection, fewer apoptotic inflammatory cells are seen in brochoalveolar lavage fluid (BALF) from Tnf-null lungs compared to wild-type BALF

immune system

lung inflammation ( J:127028 )

• intratracheal injection of bleomycin induces infiltration of inflammatory cells in lungs; infiltration reaches peak at day 7 and decreases from that time point in wild-type, but treated mutants show persistent infiltration beyond day 7 to at least day 35 after injection

respiratory system

lung inflammation ( J:127028 )

abnormal lung morphology ( J:127028 )

• Tnf-null lungs show significantly higher total cell and lymphocyte counts than wild-type at 21 days following intratracheal injection of bleomycin

• inflammatory changes (infiltration by lymphocytes, septal thickening of alveoli, and fibroblast proliferation) subside in wild-type mice by 75 days post-bleomycin treatment but severe infiltration and honeycomb-like structure are still observed in mutant lungs at this time

Allelic Composition	Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	either: B6.129-Tnf^tm1Ljo or (involves: 129 * C57BL/6)

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased leukocyte cell number ( J:97783 )

increased neutrophil cell number ( J:97783 )

• neutrophil numbers are increased compared to Tnf^tm1.2Sned homozygotes

increased lymphocyte cell number ( J:97783 )

• lymphocyte numbers are increased compared to Tnf^tm1.2Sned homozygotes

absent follicular dendritic cells ( J:97783 )

abnormal Peyer's patch follicle morphology ( J:97783 )

• absent primary B cell follicles; however, follicle-associated epithelium and distribution of M cells are normal

abnormal Peyer's patch T cell area morphology ( J:97783 )

• mixed T and B cell zones

abnormal Peyer's patch germinal center morphology ( J:97783 )

• absent germinal centers

decreased Peyer's patch number ( J:97783 )

• Peyer's patches are fewer in number

small Peyer's patches ( J:97783 )

decreased susceptibility to bacterial infection ( J:97783 )

• homozygotes are protected from LPS/D-Gal induced shock

increased susceptibility to parasitic infection ( J:97783 )

• homozygotes show increased sensitivity to systemic and alimentary L. monocytogenes infection

hematopoietic system

increased leukocyte cell number ( J:97783 )

increased neutrophil cell number ( J:97783 )

• neutrophil numbers are increased compared to Tnf^tm1.2Sned homozygotes

increased lymphocyte cell number ( J:97783 )

• lymphocyte numbers are increased compared to Tnf^tm1.2Sned homozygotes

Allelic Composition	Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	involves: 129S1/Sv

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

HIstology of liver and spleen from C. parvum-treated wild type and Tnf^tm1Ljo/Tnf^tm1Ljo mice

immune system

decreased spleen B cell follicle number ( J:95684 )

• absence of polarized B-cell follicles

absent spleen germinal center ( J:41992 , J:80616 )

• mutants fail to develop germinal centers in the spleen following immunization with SRBC (J:41992)

decreased spleen white pulp amount ( J:80616 )

• gradual reduction of white pulp size

decreased IgG level ( J:41992 )

• mutants exhibit a deficiency in maturation of normal antibody response to T-cell dependent antigen (SRBC), showing depressed IgG titers but normal IgM titers

decreased circulating tumor necrosis factor level ( J:95684 )

• serum TNF levels are reduced in mutants injected with LPS and can not be detected on day 2 of Listeria monocytogenes infection

abnormal follicular dendritic cell physiology ( J:41992 )

• the normal uptake of aggregated gamma globulin by follicular dendritic cells in the spleen of immunized mutants is absent

abnormal cytokine secretion ( J:41992 )

• production of colony-stimulating factor (CSF), specifically granulocyte-macrophage (GM)-CSF, is reduced after i.p. injection of lipopolysaccharide (LPS)

abnormal chemokine secretion ( J:95684 )

• induction of MCP-1 is reduced in spleens of mutants infected with Listeria monocytogenes

decreased susceptibility to autoimmune disorder ( J:95684 )

• increased resistance to liver injury after ConA-induced autoimmune hepatitis, with only infiltrating cells but no liver necrosis detected

decreased susceptibility to endotoxin shock ( J:41992 , J:95684 )

• mutants are resistant to the lethality of minute doses of lipopolysaccharide (LPS) following D-galactosamine treatment, however at higher concentrations (1200ug), they succumb to illness to the same extent as wild-type (J:41992)

• mutants are resistant to staphylococcal enterotoxin B in the presence of D-gal (J:41992)

• protected from S. aureus enterotoxin B induced toxic shock (J:95684)

abnormal susceptibility to infection ( J:41992 )

increased susceptibility to bacterial infection ( J:41992 , J:80616 , J:95684 )

• mutants show a delayed (40 days post injection instead of the 10-14 seen in wild-type), but progressive, response to heat-killed Corynebacterium parvum, that, unlike in wild-type, eventually leads to death by 80 days (J:41992)

• increased susceptibility to Salmonella enterica infection (J:80616)

• increased susceptibility to Listeria monocytogenes infections, with mutants dying by day 8 while wild-type mice survive (J:80616)

• loss of resistance against Listeria monocytogenes (J:95684)

increased susceptibility to fungal infection ( J:41992 )

• mutants exhibit increased susceptibility to Candida albicans challenge; 100% die by day 7 post-infection

hematopoietic system

decreased spleen B cell follicle number ( J:95684 )

• absence of polarized B-cell follicles

absent spleen germinal center ( J:41992 , J:80616 )

• mutants fail to develop germinal centers in the spleen following immunization with SRBC (J:41992)

decreased spleen white pulp amount ( J:80616 )

• gradual reduction of white pulp size

decreased IgG level ( J:41992 )

• mutants exhibit a deficiency in maturation of normal antibody response to T-cell dependent antigen (SRBC), showing depressed IgG titers but normal IgM titers

homeostasis/metabolism

decreased circulating tumor necrosis factor level ( J:95684 )

• serum TNF levels are reduced in mutants injected with LPS and can not be detected on day 2 of Listeria monocytogenes infection

Allelic Composition	Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	involves: 129S1/Sv * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased neutrophil cell number ( J:127028 )

• neutrophil numbers are similar in uninfected or infected (during first days of infection) wild-type controls and Tnf^tm2Jods homozygous mice

• 7 days post-infection, neutrophil cell number assessed by myeloperoxidase activity levels in footpad lesions relative to infected controls; higher percentage of neutrophils are detected in footpads after 7 days

abnormal neutrophil physiology ( J:127028 )

• 40 days post-infection, a higher percentage of neutrophils in lesion are apoptotic compared to infected controls

decreased immunoglobulin level ( J:127028 )

• levels of IgG2a ins sera are undetectable in infected mutants compared to infected wild-type

abnormal lymph node cell ratio ( J:127028 )

• mean percentage of IFN-gamma secreting CD4+ T cells is markedly enhanced (23.6%) in draining lymph nodes

lymph node hypoplasia ( J:127028 )

• cellularity is relatively lower than in wild-type C57BL/6 or Tnf^tm1Jods homozygous mice

increased interferon-gamma secretion ( J:127028 )

• lymph node cells release more IFN-gamma than wild-type cells

• Il-4 secretion upon re-stimulation with L. major is equivalent to wild-type

skin inflammation ( J:127028 )

increased susceptibility to parasitic infection ( J:127028 )

• 60 days after L. major infection, mutants have much higher numbers of intralesional parasites than infected wild-type C57BL/6

• number of parasitized neutrophils in lesion is significantly higher in mutants than in infected controls 4 hours after i.p. injection of L.major; percentage of proliferating parasites released from neutrophils is significantly greater than seen in infected controls

hematopoietic system

increased neutrophil cell number ( J:127028 )

• neutrophil numbers are similar in uninfected or infected (during first days of infection) wild-type controls and Tnf^tm2Jods homozygous mice

abnormal neutrophil physiology ( J:127028 )

• 40 days post-infection, a higher percentage of neutrophils in lesion are apoptotic compared to infected controls

decreased immunoglobulin level ( J:127028 )

• levels of IgG2a ins sera are undetectable in infected mutants compared to infected wild-type

integument

skin inflammation ( J:127028 )

skin lesions ( J:127028 )

• mice infected with L. major promastigotes are unable to control developing footpad lesion size, exhibiting much larger lesion size than infected wild-type control at >35 days after infection

Allelic Composition	Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	involves: 129S1/Sv * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

liver/biliary system

liver/biliary system phenotype ( J:112797 )

N	• mice fed a high cholesterol diet do not develop hepatic steatosis unlike Lta/Tnf^tm1Eug homozygotes

Allelic Composition	Tnf^tm1Ljo/Tnf⁺
Genetic Background	involves: 129S1/Sv

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased susceptibility to bacterial infection ( J:41992 )

• exhibit increased susceptibility to high-dose LPS lethality

• exhibit delayed resolution of the Corynebacterium parvum induced inflammatory process lipopolysaccharide (LPS) lethality

increased susceptibility to fungal infection ( J:41992 )

• exhibit increased susceptibility to Candida albicans challenge; 60% die by 30 days post-infection

Allelic Composition	Nfkb1^tm1Bal/Nfkb1^tm1Bal Rela^tm1Bal/Rela^tm1Bal Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	involves: 129P2/OlaHsd * 129S1/Sv * 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nfkb1^tm1Bal mutation (3 available); any Nfkb1 mutation (102 available)
Rela^tm1Bal mutation (1 available); any Rela mutation (28 available)
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:91366 )

• triple homozygous pups die within 12 hours of birth

prenatal lethality, incomplete penetrance ( J:91366 )

• fewer than expected triple homozygous mutants are found at birth

integument

thin epidermis ( J:91366 )

• the epidermis is marginally thinner

Allelic Composition	Rel^tm1Grd/Rel^tm1Grd Rela^tm1Bal/Rela^tm1Bal Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	involves: 129P2/OlaHsd * 129S1/Sv * 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rela^tm1Bal mutation (1 available); any Rela mutation (28 available)
Rel^tm1Grd mutation (2 available); any Rel mutation (45 available)
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:91366 )

• triple homozygous pups die within 12 hours of birth

cellular

abnormal cell cycle ( J:91366 )

• transit-amplifying epidermal cells display a delay in G₁/S phase progression

decreased cell proliferation ( J:91366 )

• transit-amplifying epidermal cells display reduced proliferation

embryo

decreased embryo size ( J:91366 )

• triple homozygous embryos are about 30% smaller compared to littermate controls

growth/size/body

decreased embryo size ( J:91366 )

• triple homozygous embryos are about 30% smaller compared to littermate controls

integument

abnormal hair follicle placode formation ( J:91366 )

• about a 24 hour delay in hair placode formation is seen compared to control littermates

underdeveloped hair follicles ( J:91366 )

• at E18 the hair follicles that are present are only rudimentary buds lacking hair shafts

decreased hair follicle number ( J:91366 )

• at E18, 70% fewer hair follicles are present compared to control littermates

abnormal epidermal layer morphology ( J:91366 )

abnormal epidermis stratum basale morphology ( J:91366 )

• the cells in the basal cell layer are organized differently and the basal cell profile area is reduced by about 33%

abnormal keratinocyte morphology ( J:91366 )

• the number of differentiating keratinocytes is reduced

thin epidermis ( J:91366 )

• the epidermis is significantly thinner

Allelic Composition	Rela^tm1Bal/Rela^tm1Bal Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	involves: 129S1/Sv * 129S4/SvJae * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rela^tm1Bal mutation (1 available); any Rela mutation (28 available)
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal nervous system physiology ( J:124444 )

• Schwann cell apoptosis is increased compared to in wild-type mice 24 hours post-axotomy

Allelic Composition	Tnf^tm1Ljo/Tnf^tm1Ljo Tnip1^tm1.1Ama/Tnip1^tm1.1Ama
Genetic Background	involves: 129S1/Sv * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)
Tnip1^tm1.1Ama mutation (0 available); any Tnip1 mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:145539 )

N	• mice are born in Mendelian ratio

Allelic Composition	Tnf^tm1Ljo/Tnf⁺ Tnip1^tm1.1Ama/Tnip1^tm1.1Ama
Genetic Background	involves: 129S1/Sv * C57BL/6J

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

perinatal lethality, incomplete penetrance ( J:145539 )

• fewer than expected mice are born alive

Allelic Composition	Rela^tm1Yuo/Rela^tm1Yuo Tnf^tm1Ljo/Tnf^tm1Ljo
Genetic Background	involves: 129S/SvEv * 129S1/Sv

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rela^tm1Yuo mutation (4 available); any Rela mutation (28 available)
Tnf^tm1Ljo mutation (3 available); any Tnf mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

abnormal myogenesis ( J:135692 )

• myogenesis is accelerated in myoblasts in cultured mouse embryonic fibroblasts and in vivo

decreased skeletal muscle fiber diameter ( J:135692 )

• mice exhibit a decrease in fiber diameter

increased skeletal muscle fiber number ( J:135692 )

• mutant mice exhibit a 76% increase in the number of myofibers

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24