Phenotypes associated with this allele

• Allele Symbol: Tlr4^lps-del
• Allele Name: defective lipopolysaccharide response, deletion
• Allele ID: MGI:1860755
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tlr4^lps-del/Tlr4^lps-del	C57BL/10ScCr	MGI:3588730
hm2	Tlr4^lps-del/Tlr4^lps-del	C57BL/10ScN	MGI:3834581
hm3	Tlr4^lps-del/Tlr4^lps-del	involves: C57BL/10ScN	MGI:3839781
cn4	Tg(Vil1-cre)997Gum/? Tlr4^lps-del/Tlr4^lps-del Tlr5^tm1.1Gewr/Tlr5^tm1.1Gewr	involves: C57BL/6J * C57BL/6NTac * C57BL/10ScN * SJL	MGI:5751557
cx5	Il12rb2^Ifnm-d/Il12rb2^Ifnm-d Tlr4^lps-del/Tlr4^lps-del	C57BL/10ScCr	MGI:3603338

Genotype
MGI:3588730

hm1

• Allelic Composition: Tlr4^lps-del/Tlr4^lps-del
• Genetic Background: C57BL/10ScCr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal macrophage physiology ( J:88996 )

• poor activation of macrophage by Leishmania major

abnormal cytokine secretion ( J:88996 )

• higher levels of Th1 and Th2 cytokines secreted at 4 weeks after infection with Leishmania major than in controls

increased susceptibility to bacterial infection ( J:51522 , J:88996 )

• response to endotoxin (LPS) prevented (J:51522)

• Leishmania major parasitic loads significantly higher than controls starting 24 hours after infection and persisting to 11 weeks after infection when controls are free of parasites (J:88996)

behavior/neurological

hyporesponsive to tactile stimuli ( J:97819 )

• attenuated mechanical allodynia after nerve injury

increased thermal nociceptive threshold ( J:97819 )

• attenuated response to heat

nervous system

abnormal glial cell physiology ( J:97819 )

• glial activation after nerve injury reduced

cardiovascular system

decreased response of heart to induced stress ( J:106358 )

• exhibit reduced cardiac hypertrophy following pressure overload compared to controls

homeostasis/metabolism

decreased susceptibility to injury ( J:97819 )

• reduced spinal expression of TNF alpha, IFN gamma, IL-6 and IL-1 after injury

• glial activation after nerve injury reduced

decreased response of heart to induced stress ( J:106358 )

• exhibit reduced cardiac hypertrophy following pressure overload compared to controls

hematopoietic system

abnormal macrophage physiology ( J:88996 )

• poor activation of macrophage by Leishmania major

Genotype
MGI:3834581

hm2

• Allelic Composition: Tlr4^lps-del/Tlr4^lps-del
• Genetic Background: C57BL/10ScN

digestive/alimentary system

rectal hemorrhage ( J:37271 )

• rectal bleeding after 7 days of dextran sodium sulfate treatment is reduced relative to controls

immune system

increased spleen weight ( J:37271 )

• spleen weight elevated with dextran sodium sulfate treatment relative to controls

respiratory system

enlarged lung ( J:114985 )

• significantly increased lung volume at 3 months of age in contrast to normal body weights through 12 months

abnormal pulmonary alveolar duct morphology ( J:114985 )

• enlarged air spaces distal to the terminal bronchioles

abnormal pulmonary alveolus morphology ( J:114985 )

• destruction of normal alveolar structures

skeleton

increased bone mineral content ( J:118467 )

• greater mineral content at 20-24 weeks

increased bone mass ( J:118467 )

• larger bones at 20-24 weeks of age

adipose tissue

decreased percent body fat/body weight ( J:118467 )

• 70% less body fat

vision/eye

abnormal retina rod bipolar cell morphology ( J:141070 )

• increased differentiation at around 13 days of age

• growth factor treatment enhances proliferation

abnormal retina progenitor cell morphology ( J:141070 )

• more proliferating cells in the retina at 6 days of age

• neuronal differentiation of precursors

• FGF-2 injection is required for a similar effect after 15 days of age

abnormal retina layer morphology ( J:141070 )

abnormal retina rod cell morphology ( J:141070 )

• increased differentiation of rod photoreceptors at around 13 days of age

• growth factor treatment enhances proliferation

abnormal retina pigment epithelium morphology ( J:141070 )

• increased number of proliferating cells in the retinal pigment cell layer at 6 days of age

nervous system

abnormal retina rod bipolar cell morphology ( J:141070 )

• increased differentiation at around 13 days of age

• growth factor treatment enhances proliferation

abnormal retina rod cell morphology ( J:141070 )

• increased differentiation of rod photoreceptors at around 13 days of age

• growth factor treatment enhances proliferation

pigmentation

abnormal retina pigment epithelium morphology ( J:141070 )

• increased number of proliferating cells in the retinal pigment cell layer at 6 days of age

hematopoietic system

increased spleen weight ( J:37271 )

• spleen weight elevated with dextran sodium sulfate treatment relative to controls

cardiovascular system

rectal hemorrhage ( J:37271 )

• rectal bleeding after 7 days of dextran sodium sulfate treatment is reduced relative to controls

growth/size/body

enlarged lung ( J:114985 )

• significantly increased lung volume at 3 months of age in contrast to normal body weights through 12 months

increased spleen weight ( J:37271 )

• spleen weight elevated with dextran sodium sulfate treatment relative to controls

Genotype
MGI:3839781

hm3

• Allelic Composition: Tlr4^lps-del/Tlr4^lps-del
• Genetic Background: involves: C57BL/10ScN

immune system

abnormal tumor necrosis factor level ( J:114368 )

• diminished TNF alpha expression after 90 minutes of liver ischemia followed by 6 hours of reperfusion

decreased interleukin-10 secretion ( J:147023 )

• production is suppressed in response to E coli with or without adenosine

decreased interleukin-6 secretion ( J:147023 )

• production is suppressed in response to E coli

homeostasis/metabolism

abnormal circulating enzyme level ( J:114368 )

• myeloperoxidase levels are reduced after 90 minutes of liver ischemia followed by 6 hours of reperfusion

• increased HO-1 expression

decreased circulating alanine transaminase level ( J:114368 )

• serum levels decreased after 90 minutes of liver ischemia followed by 6 hours of reperfusion

abnormal tumor necrosis factor level ( J:114368 )

• diminished TNF alpha expression after 90 minutes of liver ischemia followed by 6 hours of reperfusion

nervous system

abnormal neuron differentiation ( J:129957 )

• improved differentiation of progenitor cells into neurons

• survival of newly formed neurons is reduced

brain ischemia ( J:148091 )

• improved neurological score relative to controls after ischemia/reperfusion

• reduced infarct size relative to controls at 24 hours after ischemia/reperfusion

cellular

abnormal neuron differentiation ( J:129957 )

• improved differentiation of progenitor cells into neurons

• survival of newly formed neurons is reduced

Genotype
MGI:5751557

cn4

• Allelic Composition: Tg(Vil1-cre)997Gum/?; Tlr4^lps-del/Tlr4^lps-del; Tlr5^tm1.1Gewr/Tlr5^tm1.1Gewr
• Genetic Background: involves: C57BL/6J * C57BL/6NTac * C57BL/10ScN * SJL

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:229155 )

N • mice do not exhibit symptoms of metabolic syndrome

N • fat pad weight, body weight, spleen weight, colon weight, and fasting glucose levels are similar to wild-type

behavior/neurological

polyphagia ( J:229155 )

digestive/alimentary system

abnormal colon morphology ( J:229155 )

• increased colon weight

decreased colon length ( J:229155 )

large intestinal inflammation ( J:229155 )

• low grade inflammation, however, phenotype is less severe than in mice with wild-type Tlr4

immune system

large intestinal inflammation ( J:229155 )

• low grade inflammation, however, phenotype is less severe than in mice with wild-type Tlr4

Genotype
MGI:3603338

cx5

• Allelic Composition: Il12rb2^Ifnm-d/Il12rb2^Ifnm-d; Tlr4^lps-del/Tlr4^lps-del
• Genetic Background: C57BL/10ScCr

immune system

abnormal immune cell physiology ( J:91014 )

abnormal granulocyte physiology ( J:91014 )

• increased infiltration at sites of infection

abnormal NK cell physiology ( J:91014 )

• increased infiltration at sites of infection

abnormal macrophage physiology ( J:91014 )

• increased infiltration at sites of infection

abnormal dendritic cell physiology ( J:91014 )

• increased infiltration of myeloid derived dendritic cells at infection sites

decreased interferon-gamma secretion ( J:70821 , J:91014 )

• inability or reduced ability to produce IFN gamma in response to bacterial infection (J:91014)

increased interleukin-4 secretion ( J:91014 )

• elevated Il4 production

increased susceptibility to bacterial infection ( J:70821 , J:91014 )

• defective response to lipopolysaccharide (J:70821)

• unable to resolve Leishmania major infections (J:91014)

• develop nonhealing lesions by 33 days after infection (J:91014)

• formation of lesions slower after infection (J:91014)

• develop higher parasite loads at sites of infection (J:91014)

hematopoietic system

abnormal granulocyte physiology ( J:91014 )

• increased infiltration at sites of infection

abnormal NK cell physiology ( J:91014 )

• increased infiltration at sites of infection

abnormal macrophage physiology ( J:91014 )

• increased infiltration at sites of infection