Phenotypes associated with this allele

• Allele Symbol: Ltb^tm1Kpf
• Allele Name: targeted mutation 1, Klaus Pfeffer
• Allele ID: MGI:1860970
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ltb^tm1Kpf/Ltb^tm1Kpf	B6.129P2-Ltb^tm1Kpf	MGI:2182270
hm2	Ltb^tm1Kpf/Ltb^tm1Kpf	involves: 129P2/OlaHsd * C57BL/6	MGI:3575645
cx3	Ltb^tm1Kpf/Ltb^tm1Kpf Tnfsf14^tm1Kpf/Tnfsf14^tm1Kpf	involves: 129P2/OlaHsd * C57BL/6	MGI:4441319

Genotype
MGI:2182270

hm1

• Allelic Composition: Ltb^tm1Kpf/Ltb^tm1Kpf
• Genetic Background: B6.129P2-Ltb^tm1Kpf

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality ( J:158663 )

• all die by 10 days after infection with C. rodentium

immune system

colitis ( J:158663 )

• edema post infection with C. rodentium

abnormal class switch recombination ( J:80616 )

• specific IgG antibodies are almost undetectable in SRBC immunized mutants, indicating defective class switching

increased leukocyte cell number ( J:80616 )

• 2- to 3- fold increase in leukocyte count in spleen, blood, and peritoneal cavity

abnormal spleen morphology ( J:80616 )

• T-cell zones appear distinct, while B cells are significantly scattered

decreased spleen B cell follicle number ( J:80616 )

• absence of polarized B-cell follicles

absent spleen marginal zone ( J:80616 )

decreased spleen white pulp amount ( J:80616 )

• gradual reduction of white pulp size

decreased IgG level ( J:80616 )

• specific IgG antibodies are almost undetectable in SRBC immunized mutants

increased IgM level ( J:80616 )

• total IgM levels are increased to about 2-times that of wild-type, due to an increase in number of IgM producing plasma cells in the spleen

increased susceptibility to bacterial infection ( J:80616 , J:158663 )

• increased susceptibility to Salmonella enterica infection, but not Listeria monocytogenes (J:80616)

• display weight loss, colitis and severe disruption of the epithelial layer, ulceration, and bacterial abscesses in the colon after infection with Citrobacter rodentium (J:158663)

increased susceptibility to bacterial infection induced morbidity/mortality ( J:158663 )

• all die by 10 days after infection with C. rodentium

hematopoietic system

abnormal class switch recombination ( J:80616 )

• specific IgG antibodies are almost undetectable in SRBC immunized mutants, indicating defective class switching

increased leukocyte cell number ( J:80616 )

• 2- to 3- fold increase in leukocyte count in spleen, blood, and peritoneal cavity

abnormal spleen morphology ( J:80616 )

• T-cell zones appear distinct, while B cells are significantly scattered

decreased spleen B cell follicle number ( J:80616 )

• absence of polarized B-cell follicles

absent spleen marginal zone ( J:80616 )

decreased spleen white pulp amount ( J:80616 )

• gradual reduction of white pulp size

decreased IgG level ( J:80616 )

• specific IgG antibodies are almost undetectable in SRBC immunized mutants

increased IgM level ( J:80616 )

• total IgM levels are increased to about 2-times that of wild-type, due to an increase in number of IgM producing plasma cells in the spleen

growth/size/body

weight loss ( J:158663 )

• exhibit weight loss post infection with C. rodentium

digestive/alimentary system

abnormal colon morphology ( J:158663 )

• colon severe disruption of the epithelial layer, ulceration, and bacterial abscesses in the colon post infection with C. rodentium

colitis ( J:158663 )

• edema post infection with C. rodentium

Genotype
MGI:3575645

hm2

• Allelic Composition: Ltb^tm1Kpf/Ltb^tm1Kpf
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

increased lymphocyte cell number ( J:42508 )

• elevated numbers of lymphocytes in blood and peritoneal cavity, however no defects B-cell maturation in the bone marrow or in thymocyte maturation

abnormal spleen morphology ( J:42508 , J:58981 )

• no defect in the cellularity of spleen (J:42508)

• spleen architecture is disrupted (J:58981)

• however, an ellipsoid-like structure still defines the white pulp area (J:58981)

abnormal spleen red pulp morphology ( J:42508 )

• B cells invaded the red pulp regions

abnormal spleen follicular dendritic cell network ( J:42508 )

• impaired follicular dendritic cell network development in spleens of antigen-stimulated mice

abnormal spleen germinal center morphology ( J:42508 )

• impaired germinal center formation in spleens of antigen-stimulated mice

abnormal spleen marginal zone morphology ( J:42508 )

• disruption of marginal zones

absent spleen marginal zone ( J:58981 )

• the marginal zone in the spleen is absent

• however, B and T cell zones remain distinct

decreased spleen white pulp amount ( J:109621 )

• percentage of splenic white pulp is lower than in wild-type but higher than both Lta mutant lines, Ltrb, or Ltb/Tnf/Lta knockouts

absent follicular dendritic cells ( J:58981 )

• mature follicular dendritic cells are absent from the mesenteric lymph nodes

absent Peyer's patches ( J:42508 )

abnormal mesenteric lymph node morphology ( J:42508 , J:58981 )

• although mesenteric lymph nodes were found, they lacked distinct B cell follicles and follicular dendritic cell networks (J:42508)

• in most mice only one large spherical mesenteric lymph node is present unlike in wild-type mice that possess a chain of three elongated lymph node segments (J:58981)

• mature follicular dendritic cells are absent from the mesenteric lymph nodes (J:58981)

• the reticulum of the deep cortex is coarser than in wild-type mice and the transition between the follicular dendritic cell at the germinal-like center is less distinct than in wild-type mice (J:58981)

absent axillary lymph nodes ( J:42508 )

• lacked axillary lymph node

absent brachial lymph nodes ( J:42508 )

• lacked brachial lymph nodes

absent cervical lymph nodes ( J:58981 )

• cervical lymph nodes could not be identified

absent inguinal lymph nodes ( J:42508 )

• lacked inguinal lymph nodes

absent popliteal lymph nodes ( J:42508 )

• lacked popliteal lymph nodes

liver/biliary system

abnormal liver morphology ( J:42508 )

• accumulation of lymphocytes around perivascular areas of the liver

respiratory system

abnormal lung morphology ( J:42508 )

• accumulation of lymphocytes around perivascular areas of the lung

hematopoietic system

increased lymphocyte cell number ( J:42508 )

• elevated numbers of lymphocytes in blood and peritoneal cavity, however no defects B-cell maturation in the bone marrow or in thymocyte maturation

abnormal spleen morphology ( J:42508 , J:58981 )

• no defect in the cellularity of spleen (J:42508)

• spleen architecture is disrupted (J:58981)

• however, an ellipsoid-like structure still defines the white pulp area (J:58981)

abnormal spleen red pulp morphology ( J:42508 )

• B cells invaded the red pulp regions

abnormal spleen follicular dendritic cell network ( J:42508 )

• impaired follicular dendritic cell network development in spleens of antigen-stimulated mice

abnormal spleen germinal center morphology ( J:42508 )

• impaired germinal center formation in spleens of antigen-stimulated mice

abnormal spleen marginal zone morphology ( J:42508 )

• disruption of marginal zones

absent spleen marginal zone ( J:58981 )

• the marginal zone in the spleen is absent

• however, B and T cell zones remain distinct

decreased spleen white pulp amount ( J:109621 )

• percentage of splenic white pulp is lower than in wild-type but higher than both Lta mutant lines, Ltrb, or Ltb/Tnf/Lta knockouts

Genotype
MGI:4441319

cx3

• Allelic Composition: Ltb^tm1Kpf/Ltb^tm1Kpf; Tnfsf14^tm1Kpf/Tnfsf14^tm1Kpf
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal spleen morphology ( J:77250 )

• abnormal spleen architecture, with T cells accumulating around the central arteriole surrounded by a wall of B cells

absent Peyer's patches ( J:77250 )

abnormal mesenteric lymph node morphology ( J:77250 )

• only 4 out of 16 mutants have mesenteric lymph nodes

absent axillary lymph nodes ( J:77250 )

absent brachial lymph nodes ( J:77250 )

absent inguinal lymph nodes ( J:77250 )

absent popliteal lymph nodes ( J:77250 )

absent lymph nodes ( J:77250 )

• absent brachial, axillary, inguinal, popliteal, paraaortic, and parapancreatic lymph nodes

hematopoietic system

abnormal spleen morphology ( J:77250 )

• abnormal spleen architecture, with T cells accumulating around the central arteriole surrounded by a wall of B cells