Phenotypes associated with this allele

• Allele Symbol: Itgb2^tm2Bay
• Allele Name: targeted mutation 2, Baylor College of Medicine
• Allele ID: MGI:1861705
• Summary: 11 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Itgb2^tm2Bay/Itgb2^tm2Bay	B6.129S7-Itgb2^tm2Bay	MGI:3758482
hm2	Itgb2^tm2Bay/Itgb2^tm2Bay	involves: 129S7/SvEvBrd	MGI:3583104
hm3	Itgb2^tm2Bay/Itgb2^tm2Bay	involves: 129S7/SvEvBrd * C57BL/6	MGI:3766952
hm4	Itgb2^tm2Bay/Itgb2^tm2Bay	involves: 129S7/SvEvBrd * C57BL/6J	MGI:2651618
hm5	Itgb2^tm2Bay/Itgb2^tm2Bay	involves: 129S7/SvEvBrd * PL/J	MGI:3590414
hm6	Itgb2^tm2Bay/Itgb2^tm2Bay	PL.129S7-Itgb2^tm2Bay	MGI:5555032
ht7	Itgb2^tm1Bay/Itgb2^tm2Bay	B6.129S7-Itgb2^tm1Bay Itgb2^tm2Bay	MGI:3590494
ht8	Itgb2^tm1Bay/Itgb2^tm2Bay	involves: 129S7/SvEvBrd * C57BL/6J * PL/J	MGI:3590487
ht9	Itgb2^tm1Bay/Itgb2^tm2Bay	PL.129S7-Itgb2^tm1Bay Itgb2^tm2Bay	MGI:3590415
cn10	Itgav^tm1Blb/Itgav^tm1Blb Itgb1^tm1Ref/Itgb1^tm1Ref Itgb2^tm2Bay/Itgb2^tm2Bay Itgb7^tm1Cgn/Itgb7^tm1Cgn Tg(Mx1-cre)1Cgn/0	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6 * CBA	MGI:4358370
cx11	Rhoh^tm1Brak/Rhoh^tm1Brak Itgb2^tm2Bay/Itgb2^tm2Bay	involves: 129/Sv * C57BL/6	MGI:3826459

Genotype
MGI:3758482

hm1

• Allelic Composition: Itgb2^tm2Bay/Itgb2^tm2Bay
• Genetic Background: B6.129S7-Itgb2^tm2Bay

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Skeletal abnormalities in Itgb2^tm2Bay/Itgb2^tm2Bay mice

skeleton

abnormal long bone metaphysis morphology ( J:101407 )

• the distal femur metaphysis shows decreased bone volume, trabecular bone number, and trabecular bone thickness, and an increased trabecular bone space

abnormal bone structure ( J:101407 )

decreased bone mineral density of femur ( J:101407 )

• bone mineral density is decreased in femurs at both 5 and 15 weeks of age

decreased trabecular bone mass ( J:101407 )

• decrease in trabecular bone in the distal metaphysis of femurs

osteoporosis ( J:101407 )

• although homozygotes show normal skeletal development, they exhibit features of osteoporosis

decreased bone mineralization ( J:101407 )

• bone marrow stromal stem cell (BMSSC) differentiation is compromised as indicated by a decrease in the induction of mineralization in vitro

decreased bone ossification ( J:101407 )

• although osteoclastic activity and osteoclast numbers are normal, homozygotes show decreased bone formation

immune system

abnormal leukocyte migration ( J:83226 )

• leukocyte influx in a subcutaneous air pouch in response to TNF-alpha is reduced by 59%

abnormal leukocyte adhesion ( J:83226 )

• adhesion of neutrophils to ICAM-1 or endothelial cells is not increased with Zas stimulation as observed with wild-type neutrophils

• activated neutrophils show a large decrease in adhesion in response to shear stress

increased leukocyte cell number ( J:83226 )

increased eosinophil cell number ( J:83226 )

increased neutrophil cell number ( J:83226 )

increased lymphocyte cell number ( J:83226 )

increased monocyte cell number ( J:83226 )

decreased susceptibility to bacterial infection ( J:94855 )

• mice clear a PspA⁺ strain of bacterial pneumonia faster than controls

• mice make higher than normal levels of protective anti-pneumococcal antibodies

hematopoietic system

abnormal leukocyte migration ( J:83226 )

• leukocyte influx in a subcutaneous air pouch in response to TNF-alpha is reduced by 59%

abnormal leukocyte adhesion ( J:83226 )

• adhesion of neutrophils to ICAM-1 or endothelial cells is not increased with Zas stimulation as observed with wild-type neutrophils

• activated neutrophils show a large decrease in adhesion in response to shear stress

increased leukocyte cell number ( J:83226 )

increased eosinophil cell number ( J:83226 )

increased neutrophil cell number ( J:83226 )

increased lymphocyte cell number ( J:83226 )

increased monocyte cell number ( J:83226 )

cellular

abnormal leukocyte migration ( J:83226 )

• leukocyte influx in a subcutaneous air pouch in response to TNF-alpha is reduced by 59%

abnormal leukocyte adhesion ( J:83226 )

• adhesion of neutrophils to ICAM-1 or endothelial cells is not increased with Zas stimulation as observed with wild-type neutrophils

• activated neutrophils show a large decrease in adhesion in response to shear stress

Genotype
MGI:3583104

hm2

• Allelic Composition: Itgb2^tm2Bay/Itgb2^tm2Bay
• Genetic Background: involves: 129S7/SvEvBrd

immune system

decreased T cell proliferation ( J:90954 )

• reduced staphylococcal enterotoxin A (SEA)-induced T cell proliferation in splenocytes compared to wild-type and was even more decreased than in homozygous Itgal mutant mice

hematopoietic system

decreased T cell proliferation ( J:90954 )

• reduced staphylococcal enterotoxin A (SEA)-induced T cell proliferation in splenocytes compared to wild-type and was even more decreased than in homozygous Itgal mutant mice

cellular

decreased T cell proliferation ( J:90954 )

• reduced staphylococcal enterotoxin A (SEA)-induced T cell proliferation in splenocytes compared to wild-type and was even more decreased than in homozygous Itgal mutant mice

Genotype
MGI:3766952

hm3

• Allelic Composition: Itgb2^tm2Bay/Itgb2^tm2Bay
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

immune system

increased neutrophil cell number ( J:112286 )

• circulating neutrophils are increased in mutants and this increases with age of mice

impaired neutrophil recruitment ( J:112286 )

• defect in acute neutrophil emigration in older mice is observed

decreased acute inflammation ( J:112286 )

• in response to croton oil application to the ear in young mice (7-14 weeks), inflammation is reduced

hematopoietic system

increased neutrophil cell number ( J:112286 )

• circulating neutrophils are increased in mutants and this increases with age of mice

impaired neutrophil recruitment ( J:112286 )

• defect in acute neutrophil emigration in older mice is observed

integument

spontaneous skin ulceration ( J:112286 )

• mice develop spontaneous skin lesions characterized by mononuclear leukocytes

Genotype
MGI:2651618

hm4

• Allelic Composition: Itgb2^tm2Bay/Itgb2^tm2Bay
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J

Facial and neck alopecia with ulcerative dermatitis and conjunctivitis of the eye in Itgb2^tm2Bay/Itgb2^tm2Bay mice

mortality/aging

perinatal lethality, incomplete penetrance ( J:48542 )

• 10 - 40% of newborns died perinatally

immune system

abnormal leukocyte physiology ( J:48542 )

abnormal T cell proliferation ( J:48542 )

• impaired T cell receptor (TCR)-dependent T cell proliferation

abnormal neutrophil physiology ( J:48542 )

• impaired neutrophil extravasation into the interstitial tissue

• stimulated neutrophils did not adhere to KLH-coated glass and observed no increase in cell adhesion and no decrease in the number of rolling cells upon fMLP stimulation

increased immunoglobulin level ( J:48542 )

increased IgA level ( J:48542 )

• modestly increased at 2.5-3 months of age

increased IgG level ( J:48542 )

• serum total IgG, IgG1, IgG2a, and IgG2b concentrations were increased 10-15-fold at 2.5-3 months of age and IgG3 was modestly increased

increased IgM level ( J:48542 )

• modestly increased at 2.5-3 months of age

increased circulating interleukin-3 level ( J:48542 )

• serum level of IL-3 was increased by more than 20-fold

increased circulating interleukin-6 level ( J:48542 )

• serum level of IL-6 was increased by more than 20-fold

abnormal immune system cell morphology ( J:48542 )

myeloid hyperplasia ( J:48542 )

abnormal neutrophil morphology ( J:48542 )

• neutrophils showed hypersegmentation and appendix formation of the nuclei

increased neutrophil cell number ( J:48542 )

• increased leukocyte counts were largely due to elevated numbers of circulating neutrophils

increased leukocyte cell number ( J:48542 )

• numbers of circulating leukocytes were increased up to six-fold

increased lymphocyte cell number ( J:48542 )

increased monocyte cell number ( J:48542 )

abnormal lymph organ size ( J:48542 )

enlarged spleen ( J:48542 )

• due to a myeloid hyperplasia within the red pulp with weight increased by 2.5-fold

enlarged lymph nodes ( J:48542 )

• reactive lymphadenopathy with an accumulation of plasma cells

increased inflammatory response ( J:48542 )

• occasional inflammatory lesions were observed in the heart, salivary gland, liver, and intestine

conjunctivitis ( J:48542 )

dermatitis ( J:48542 )

• surviving mice developed extended facial and submandibular ulcerative dermatitis at about 3 months of age, with the dermal inflammatory cell infiltrate consisting of eosinophils, lymphocytes, histiocytes, and occasional perivascular plasma cells, and fewer neutrophils than expected

increased susceptibility to bacterial infection ( J:48542 )

• increased susceptibility to death from S.pneumoniae infection, with no survival by day 10 of infection

vision/eye

conjunctivitis ( J:48542 )

hematopoietic system

enlarged spleen ( J:48542 )

• due to a myeloid hyperplasia within the red pulp with weight increased by 2.5-fold

myeloid hyperplasia ( J:48542 )

increased bone marrow cell number ( J:48542 )

abnormal neutrophil morphology ( J:48542 )

• neutrophils showed hypersegmentation and appendix formation of the nuclei

increased neutrophil cell number ( J:48542 )

• increased leukocyte counts were largely due to elevated numbers of circulating neutrophils

increased leukocyte cell number ( J:48542 )

• numbers of circulating leukocytes were increased up to six-fold

increased lymphocyte cell number ( J:48542 )

increased monocyte cell number ( J:48542 )

abnormal leukocyte physiology ( J:48542 )

abnormal T cell proliferation ( J:48542 )

• impaired T cell receptor (TCR)-dependent T cell proliferation

abnormal neutrophil physiology ( J:48542 )

• impaired neutrophil extravasation into the interstitial tissue

• stimulated neutrophils did not adhere to KLH-coated glass and observed no increase in cell adhesion and no decrease in the number of rolling cells upon fMLP stimulation

increased immunoglobulin level ( J:48542 )

increased IgA level ( J:48542 )

• modestly increased at 2.5-3 months of age

increased IgG level ( J:48542 )

• serum total IgG, IgG1, IgG2a, and IgG2b concentrations were increased 10-15-fold at 2.5-3 months of age and IgG3 was modestly increased

increased IgM level ( J:48542 )

• modestly increased at 2.5-3 months of age

homeostasis/metabolism

increased circulating interleukin-3 level ( J:48542 )

• serum level of IL-3 was increased by more than 20-fold

increased circulating interleukin-6 level ( J:48542 )

• serum level of IL-6 was increased by more than 20-fold

integument

dermatitis ( J:48542 )

• surviving mice developed extended facial and submandibular ulcerative dermatitis at about 3 months of age, with the dermal inflammatory cell infiltrate consisting of eosinophils, lymphocytes, histiocytes, and occasional perivascular plasma cells, and fewer neutrophils than expected

alopecia ( J:48542 )

• surviving mice developed facial alopecia

epidermal hyperplasia ( J:48542 )

reddish skin ( J:48542 )

• surviving mice developed reddening of the skin

cellular

abnormal T cell proliferation ( J:48542 )

• impaired T cell receptor (TCR)-dependent T cell proliferation

growth/size/body

enlarged spleen ( J:48542 )

• due to a myeloid hyperplasia within the red pulp with weight increased by 2.5-fold

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
leukocyte adhesion deficiency 1		DOID:0110910	OMIM:116920	J:48542

Genotype
MGI:3590414

hm5

• Allelic Composition: Itgb2^tm2Bay/Itgb2^tm2Bay
• Genetic Background: involves: 129S7/SvEvBrd * PL/J

integument

integument phenotype ( J:84126 )

N • do not develop chronic inflammatory skin disease

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
psoriasis		DOID:8893	OMIM:PS177900	J:84126

Genotype
MGI:5555032

hm6

• Allelic Composition: Itgb2^tm2Bay/Itgb2^tm2Bay
• Genetic Background: PL.129S7-Itgb2^tm2Bay

immune system

decreased susceptibility to type IV hypersensitivity reaction ( J:109598 )

• oxazolone treatment does not result in induction of allergic contact dermatitis as in wild-type mice and only a slight edema without inflammatory cells is seen or increase in T cells

integument

integument phenotype ( J:109598 )

N • mice do not develop psoriasiform dermatitis during a 2 year observation period

Genotype
MGI:3590494

ht7

• Allelic Composition: Itgb2^tm1Bay/Itgb2^tm2Bay
• Genetic Background: B6.129S7-Itgb2^tm1Bay Itgb2^tm2Bay

integument

integument phenotype ( J:84126 )

N • Background Sensitivity: compound heterozygotes do not develop chronic inflammatory skin disease like on the PL/J or mixed 129S7/SvEvBrd, C57BL/6J and PL/J background

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
NOT	psoriasis	DOID:8893	OMIM:PS177900	J:84126

Genotype
MGI:3590487

ht8

• Allelic Composition: Itgb2^tm1Bay/Itgb2^tm2Bay
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J * PL/J

immune system

dermatitis ( J:84126 )

• Background Sensitivity: mild, predominantly lymphocytic inflammatory cell response located primarily in the superficial dermis and the basal layer of the epidermis

integument

dermatitis ( J:84126 )

• Background Sensitivity: mild, predominantly lymphocytic inflammatory cell response located primarily in the superficial dermis and the basal layer of the epidermis

hyperkeratosis ( J:84126 )

acanthosis ( J:84126 )

abnormal skin condition ( J:84126 )

• Background Sensitivity: compound heterozygous mice develop chronic inflammatory skin disease on the mixed PL/J, 129S7/SvEvBrd, and C57BL/6J background but not on a C57BL/6J background

psoriasis ( J:84126 )

• 40% of (PL/J X C57BL/6J)F1 compound heterozygotes develop mild psoriasifrom dermatitis

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
psoriasis		DOID:8893	OMIM:PS177900	J:84126

Genotype
MGI:3590415

ht9

• Allelic Composition: Itgb2^tm1Bay/Itgb2^tm2Bay
• Genetic Background: PL.129S7-Itgb2^tm1Bay Itgb2^tm2Bay

immune system

dermatitis ( J:84126 )

• Background Sensitivity: develop moderate to severe psoriasiform dermatitis on the PL/J background but not on the C57BL/6J background

integument

dermatitis ( J:84126 )

• Background Sensitivity: develop moderate to severe psoriasiform dermatitis on the PL/J background but not on the C57BL/6J background

orthokeratosis ( J:84126 )

parakeratosis ( J:84126 )

acanthosis ( J:84126 )

abnormal skin condition ( J:84126 )

• Background Sensitivity: compound heterozygous mice develop chronic inflammatory skin disease on the PL/J background but not a C57BL/6J background

psoriasis ( J:84126 )

• lesions are multifocal or patchy

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
psoriasis		DOID:8893	OMIM:PS177900	J:84126

Genotype
MGI:4358370

cn10

• Allelic Composition: Itgav^tm1Blb/Itgav^tm1Blb; Itgb1^tm1Ref/Itgb1^tm1Ref; Itgb2^tm2Bay/Itgb2^tm2Bay; Itgb7^tm1Cgn/Itgb7^tm1Cgn; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6 * CBA

immune system

immune system phenotype ( J:134784 )

N • following pIpC-induction, dendritic cells transplanted into the footpad exhibit normal drainage into lymph nodes

abnormal cellular extravasation ( J:134784 )

• abolished in pIpC-induced mice

abnormal dendritic cell physiology ( J:134784 )

• following pIpC-induction, dendritic cells fail to adhere to and migrate on a 2-D substrate unlike wild-type dendritic cells

• however, dendritic cells from pIpC-induced mice exhibit normal migration in 3D matrix

cellular

abnormal cellular extravasation ( J:134784 )

• abolished in pIpC-induced mice

hematopoietic system

abnormal cellular extravasation ( J:134784 )

• abolished in pIpC-induced mice

Genotype
MGI:3826459

cx11

• Allelic Composition: Rhoh^tm1Brak/Rhoh^tm1Brak; Itgb2^tm2Bay/Itgb2^tm2Bay
• Genetic Background: involves: 129/Sv * C57BL/6

immune system

increased double-negative T cell number ( J:143161 )

decreased double-positive T cell number ( J:143161 )

decreased CD4-positive, alpha-beta T cell number ( J:143161 )

decreased CD8-positive, alpha-beta T cell number ( J:143161 )

decreased single-positive T cell number ( J:143161 )

hematopoietic system

increased double-negative T cell number ( J:143161 )

decreased double-positive T cell number ( J:143161 )

decreased CD4-positive, alpha-beta T cell number ( J:143161 )

decreased CD8-positive, alpha-beta T cell number ( J:143161 )

decreased single-positive T cell number ( J:143161 )