Phenotypes associated with this allele

• Allele Symbol: Dll1^tm1Gos
• Allele Name: targeted mutation 1, Achim Gossler
• Allele ID: MGI:1861919
• Summary: 10 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dll1^tm1Gos/Dll1^tm1Gos	involves: 129S1/Sv * 129X1/SvJ	MGI:2173253
hm2	Dll1^tm1Gos/Dll1^tm1Gos	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:2449029
ht3	Dll1^tm1Gos/Dll1^+	C3Fe.129-Dll1^tm1Gos	MGI:4821284
ht4	Dll1^tm1Gos/Dll1^+	involves: 129S1/Sv * 129X1/SvJ	MGI:4821285
ht5	Dll1^tm1Gos/Dll1^tm2Gos	involves: 129S1/Sv * 129X1/SvJ	MGI:3848986
ht6	Dll1^tm1Gos/Dll1^tm2Gos	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:3776428
cx7	Dll1^tm1Gos/Dll1^tm2Gos Jag2^tm1Grid/Jag2^tm1Grid	involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6J	MGI:3776435
cx8	Dll1^tm1Gos/Dll1^+ Jag2^tm1Grid/Jag2^tm1Grid	involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6J	MGI:3776434
cx9	Dll1^tm1Gos/Dll1^+ Jag2^tm1Grid/Jag2^+	involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6J	MGI:3776431
cx10	Notch2^tm1Grid/Notch2^+ Dll1^tm1Gos/Dll1^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:3583235

Genotype
MGI:2173253

hm1

• Allelic Composition: Dll1^tm1Gos/Dll1^tm1Gos
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:156172 )

• die at E12

nervous system

abnormal neuron differentiation ( J:156172 )

• overproduction of Isl1/Isl2+ motoneurons at E9.5 in the intermediate and ventral neural tube

• increase in the number of Chx10+ V2a, Gata3+ V2b and Evx1/Evx2+ V0 neurons at E10.5 in the intermediate and ventral neural tube

• expression analysis indicates and increase in the pace of neurogenesis

decreased neuronal precursor cell number ( J:156172 )

• about a 40 - 50% reduction in the number of progenitor cells in the p0 domain of the spinal cord at E10.5

• progressive depletion of motorneuron progenitors over time

increased neuron number ( J:156172 )

• increase in the number of Chx10+ V2a, Gata3+ V2b and Evx1/Evx2+ V0 neurons at E10.5 in the intermediate and ventral neural tube

increased motor neuron number ( J:156172 )

• overproduction of Isl1/Isl2+ motoneurons at E9.5 in the intermediate and ventral neural tube

cellular

abnormal neuron differentiation ( J:156172 )

• overproduction of Isl1/Isl2+ motoneurons at E9.5 in the intermediate and ventral neural tube

• increase in the number of Chx10+ V2a, Gata3+ V2b and Evx1/Evx2+ V0 neurons at E10.5 in the intermediate and ventral neural tube

• expression analysis indicates and increase in the pace of neurogenesis

Genotype
MGI:2449029

hm2

• Allelic Composition: Dll1^tm1Gos/Dll1^tm1Gos
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:39633 )

• death around E12

embryo

abnormal direction of embryo turning ( J:80018 )

• tail rotation to the left in about 48% of embryos but random relative to heart looping

abnormal paraxial mesoderm morphology ( J:39633 )

• severe patterning defects in paraxial mesoderm

enlarged floor plate ( J:80018 )

• enlarged floor plate between E8.5 and E10.5

abnormal notochord morphology ( J:80018 )

• some regions appear as a sheet of cells associated with the dorsal primitive gut endoderm

• reduced number of notochord cells

abnormal primitive node morphology ( J:80018 )

• rupture of surface, bulging of cells

• by late headfold stage, abnormal cell morphology disrupts symmetry

absent embryonic cilia ( J:80018 )

• loss of monociliated cells

abnormal somite development ( J:39633 )

abnormal somite border morphology ( J:39633 )

• myoblasts span segment (somite) borders, indicating that borders are not maintained

abnormal somite shape ( J:39633 )

• irregularly shaped segments in the trunk

• distinct dermatomes present at E10.5 but segmental arrangement of myotome and sclerotome cells is disturbed

• myoblasts sometimes span space between adjacent segments

incomplete somite formation ( J:39633 )

• absence of segments in tail bud

• caudal sclerotome forms a uniform, loose, non segmental mass

nervous system

abnormal neuron differentiation ( J:39633 )

• excessive neuronal differentiation in CNS

enlarged floor plate ( J:80018 )

• enlarged floor plate between E8.5 and E10.5

abnormal brain development ( J:39633 )

• hyperplastic CNS

abnormal somatic nervous system morphology ( J:39633 )

fused dorsal root ganglion ( J:39633 )

abnormal spinal nerve morphology ( J:39633 )

• wide

• irregularly spaced

cardiovascular system

abnormal direction of heart looping ( J:80018 )

• heart looping to the left in about 50% of embryos

• looping is sometimes incomplete at E8.5-9.5

hemorrhage ( J:39633 )

• severely hemorrhagic after E10

endocrine/exocrine glands

abnormal pancreas development ( J:57072 )

• pancreatic bud decreased in size

• composed primarily of endocrine cells

cellular

absent embryonic cilia ( J:80018 )

• loss of monociliated cells

abnormal neuron differentiation ( J:39633 )

• excessive neuronal differentiation in CNS

Genotype
MGI:4821284

ht3

• Allelic Composition: Dll1^tm1Gos/Dll1⁺
• Genetic Background: C3Fe.129-Dll1^tm1Gos

immune system

decreased B-1 B cell number ( J:150183 )

• in female mice

increased B cell number ( J:150183 )

• in female, but not male, mice

decreased CD4-positive, alpha-beta T cell number ( J:150183 )

• in female, but not male, mice

decreased CD8-positive, alpha-beta T cell number ( J:150183 )

• female mice exhibit a decrease in CD8alpha,beta T cells compared with wild-type mice

increased CD8-positive, alpha-beta T cell number ( J:150183 )

• male mice exhibit an increase in CD8alpha,beta T cells compared with wild-type mice

decreased IgA level ( J:150183 )

• in female mice

decreased IgG1 level ( J:150183 )

• in female mice

decreased IgG2b level ( J:150183 )

• in female mice

decreased IgG3 level ( J:150183 )

• in female mice

homeostasis/metabolism

decreased blood uric acid level ( J:150183 )

decreased circulating cholesterol level ( J:150183 )

• in male mice

decreased circulating triglyceride level ( J:150183 )

decreased circulating total protein level ( J:150183 )

decreased basal metabolism ( J:150183 )

• mice exhibit reduced metabolized energy compared with wild-type mice

increased basal metabolism ( J:150183 )

• Background Sensitivity: when normalized to individual body weight, male mice congenic on a C3 background exhibit increased metabolized energy while male mice exhibit only a tendency compared with wild-type mice whereas on a 129 background only female mice exhibit increased metabolized energy compared with wild-type mice

abnormal enzyme/coenzyme activity ( J:150183 )

• alpha-amylase activity is decreased compared to in wild-type mice

skeleton

decreased lumbar vertebrae number ( J:150183 )

• in one mouse

decreased bone mineral content ( J:150183 )

• whole body bone mineral content is reduced in female mice and tends to be reduced in male mice compared with wild-type mice

• however, bone content related to body weight is normal

decreased bone mineral density ( J:150183 )

• whole body bone mineral density is reduced compared to in wild-type mice

increased bone mineral density ( J:150183 )

• specific bone mineral density is increased compared to in wild-type mice

• however, bone content related to body weight is normal

growth/size/body

decreased lean body mass ( J:150183 )

• average body lean body mass and the average fat to lean mass ratio are reduced compared to in wild-type mice

decreased body weight ( J:150183 )

• at 6 weeks, average body weight is decreased compared with wild-type mice

• average fat-free dry mass is reduced compared to in wild-type mice

• however, body weight normalized to energy uptake is normal

decreased body height ( J:150183 )

• mice are shorter than wild-type mice

increased susceptibility to weight loss ( J:150183 )

• mice fasted for 2 days exhibit a greater loss of body weight compared with similarly treated wild-type mice

cardiovascular system

decreased heart rate ( J:150183 )

• pulse and heart rate in female mice are decreased compared to in wild-type mice

abnormal Q wave ( J:150183 )

• the Q amplitude is increased compared to in wild-type mice

increased mean systemic arterial blood pressure ( J:150183 )

• at 14 weeks in male mice

increased systemic arterial diastolic blood pressure ( J:150183 )

• at 14 weeks in male mice

increased systemic arterial systolic blood pressure ( J:150183 )

• at 14 weeks in male mice

adipose tissue

decreased total body fat amount ( J:150183 )

• average body fat content and the average fat to lean mass ratio are reduced compared to in wild-type mice

behavior/neurological

decreased food intake ( J:150183 )

• mice consume less food and have a reduced energy uptake compared with wild-type mice

decreased locomotor activity ( J:150183 )

• mice exhibit a tendency towards reduced locomotor activity compared with wild-type mice

digestive/alimentary system

abnormal defecation ( J:150183 )

• mice produce less feces than wild-type mice

hematopoietic system

decreased B-1 B cell number ( J:150183 )

• in female mice

increased B cell number ( J:150183 )

• in female, but not male, mice

decreased CD4-positive, alpha-beta T cell number ( J:150183 )

• in female, but not male, mice

decreased CD8-positive, alpha-beta T cell number ( J:150183 )

• female mice exhibit a decrease in CD8alpha,beta T cells compared with wild-type mice

increased CD8-positive, alpha-beta T cell number ( J:150183 )

• male mice exhibit an increase in CD8alpha,beta T cells compared with wild-type mice

decreased IgA level ( J:150183 )

• in female mice

decreased IgG1 level ( J:150183 )

• in female mice

decreased IgG2b level ( J:150183 )

• in female mice

decreased IgG3 level ( J:150183 )

• in female mice

Genotype
MGI:4821285

ht4

• Allelic Composition: Dll1^tm1Gos/Dll1⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

immune system

increased B cell number ( J:150183 )

• in female but not male mice

decreased CD4-positive, alpha-beta T cell number ( J:150183 )

• a non-significant reduction

homeostasis/metabolism

increased basal metabolism ( J:150183 )

• Background Sensitivity: when normalized to individual body weight, female mice on a 129 background exhibit increased metabolized energy while male mice exhibit only a tendency compared with wild-type mice whereas on a congenic C3 background only male mice exhibit increased metabolized energy compared with wild-type mice

growth/size/body

increased susceptibility to weight loss ( J:150183 )

• mice fasted for 2 days exhibit a greater loss of body weight compared with similarly treated wild-type mice

skeleton

abnormal bone mineral density ( J:150183 )

• similar to on a C3 background, mice exhibit abnormal bone mineral density compared with wild-type mice

hematopoietic system

increased B cell number ( J:150183 )

• in female but not male mice

decreased CD4-positive, alpha-beta T cell number ( J:150183 )

• a non-significant reduction

Genotype
MGI:3848986

ht5

• Allelic Composition: Dll1^tm1Gos/Dll1^tm2Gos
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

perinatal lethality ( J:148902 )

cardiovascular system

artery stenosis ( J:148902 )

• lumens of large arteries show a reduction in diameters at E18.5, with no significant differences in arterial wall thickness

aorta stenosis ( J:148902 )

• lumen shows a reduction in diameter compared to controls

abnormal capillary branching pattern ( J:148902 )

• significant increase in capillary branch points in the skin is observed at E17.5

abnormal skin vasculature morphology ( J:148902 )

• significant increase in capillary branch points in the skin is observed at E17.5

muscle

abnormal muscle morphology ( J:148902 )

abnormal myogenesis ( J:148902 )

decreased skeletal muscle mass ( J:148902 )

integument

abnormal skin vasculature morphology ( J:148902 )

• significant increase in capillary branch points in the skin is observed at E17.5

Genotype
MGI:3776428

ht6

• Allelic Composition: Dll1^tm1Gos/Dll1^tm2Gos
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

perinatal lethality, complete penetrance ( J:119082 )

• mice survive until birth

• mice do not survive postnatally

• however, mice survive beyond E10.5 when Dll1^tm1Gos homozygotes die

cellular

abnormal myoblast differentiation ( J:119082 )

• progenitor cells are lost due to premature differentiation and not an increase in apoptosis

• dermomyotome-derived myoblasts differentiate more rapidly than in wild-type mice

abnormal myotube differentiation ( J:119082 )

• beginning at E14.5, the number of secondary myotubes are severely reduced

muscle

abnormal myoblast differentiation ( J:119082 )

• progenitor cells are lost due to premature differentiation and not an increase in apoptosis

• dermomyotome-derived myoblasts differentiate more rapidly than in wild-type mice

abnormal myotube differentiation ( J:119082 )

• beginning at E14.5, the number of secondary myotubes are severely reduced

decreased skeletal muscle mass ( J:119082 )

• at E13.5, skeletal muscle is hypotrophied

• at E18.5, skeletal muscle is severely reduced

behavior/neurological

no spontaneous movement ( J:119082 )

• mice are motionless at E18.5

embryo

abnormal somite development ( J:132241 )

• mice exhibit defects in segmentation due to disrupted somitogenesis

skeleton

abnormal axial skeleton morphology ( J:119082 )

growth/size/body

decreased body length ( J:119082 )

Genotype
MGI:3776435

cx7

• Allelic Composition: Dll1^tm1Gos/Dll1^tm2Gos; Jag2^tm1Grid/Jag2^tm1Grid
• Genetic Background: involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6J

hearing/vestibular/ear

abnormal organ of Corti morphology ( J:132241 )

• unlike in wild-type mice, cells within the organ of Corti continue to proliferate between E14.5 and E17.5

abnormal cochlear hair cell morphology ( J:132241 )

• hair cells are disorganized and very densely packed

increased cochlear inner hair cell number ( J:132241 )

• cochlea exhibit a greater increase in inner hair cells than in Jag2^tm1Grid homozygotes

increased cochlear outer hair cell number ( J:132241 )

abnormal orientation of cochlear hair cell stereociliary bundles ( J:132241 )

• hair cell stereocilia bundles exhibit a lose of polarity and disorganization

decreased organ of Corti supporting cell number ( J:132241 )

• many of the missing supporting cells are derived from the Deiter's cell population

nervous system

abnormal cochlear hair cell morphology ( J:132241 )

• hair cells are disorganized and very densely packed

increased cochlear inner hair cell number ( J:132241 )

• cochlea exhibit a greater increase in inner hair cells than in Jag2^tm1Grid homozygotes

increased cochlear outer hair cell number ( J:132241 )

abnormal orientation of cochlear hair cell stereociliary bundles ( J:132241 )

• hair cell stereocilia bundles exhibit a lose of polarity and disorganization

Genotype
MGI:3776434

cx8

• Allelic Composition: Dll1^tm1Gos/Dll1⁺; Jag2^tm1Grid/Jag2^tm1Grid
• Genetic Background: involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6J

hearing/vestibular/ear

abnormal organ of Corti morphology ( J:132241 )

• cells within the organ of Corti, including pillar cells, Dieter's cells and Hensen's cells, continue to proliferate between E14.5 and E17.5 unlike in wild-type mice

increased cochlear inner hair cell number ( J:132241 )

• cochlea exhibit a greater increase in inner hair cells than in Jag2^tm1Grid homozygotes

nervous system

increased cochlear inner hair cell number ( J:132241 )

• cochlea exhibit a greater increase in inner hair cells than in Jag2^tm1Grid homozygotes

Genotype
MGI:3776431

cx9

• Allelic Composition: Dll1^tm1Gos/Dll1⁺; Jag2^tm1Grid/Jag2⁺
• Genetic Background: involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6J

hearing/vestibular/ear

increased cochlear inner hair cell number ( J:132241 )

• cochlea possess increased inner hair cells but not as many as in Jag2^tm1Grid homozygotes

increased cochlear outer hair cell number ( J:132241 )

nervous system

increased cochlear inner hair cell number ( J:132241 )

• cochlea possess increased inner hair cells but not as many as in Jag2^tm1Grid homozygotes

increased cochlear outer hair cell number ( J:132241 )

Genotype
MGI:3583235

cx10

• Allelic Composition: Notch2^tm1Grid/Notch2⁺; Dll1^tm1Gos/Dll1⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

renal/urinary system

renal/urinary system phenotype ( J:67157 )

N • no kidney defects were observed despite expression of both genes in the developing glomerulus