All Phenotypes Cd47<tm1Fpl> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd47^tm1Fpl/Cd47^tm1Fpl	B6.129S7-Cd47^tm1Fpl	MGI:4430388
hm2	Cd47^tm1Fpl/Cd47^tm1Fpl	C.129S7-Cd47^tm1Fpl	MGI:4939896
hm3	Cd47^tm1Fpl/Cd47^tm1Fpl	involves: 129S7/SvEvBrd	MGI:2180896
hm4	Cd47^tm1Fpl/Cd47^tm1Fpl	involves: 129S7/SvEvBrd * C57BL/6	MGI:4939899
hm5	Cd47^tm1Fpl/Cd47^tm1Fpl	NOD.129S7-Cd47^tm1Fpl	MGI:4939898
ht6	Cd47^tm1Fpl/Cd47⁺	B6.129S7-Cd47^tm1Fpl	MGI:2180897
cx7	Rag2^tm1Fwa/Rag2^tm1Fwa Cd47^tm1Fpl/Cd47^tm1Fpl Il2rg^tm1Cgn/Y	involves: 129P2/OlaHsd * 129S/SvEv * 129S7/SvEvBrd * C57BL/6	MGI:5538575

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality ( J:36489 )

• following i.p. injection of 5 x 10⁴ virulent E.coli (018:K1:H7), all 8-week-old homozygotes die at 2 days after challenge, whereas all heterozygous littermates survive the infection

immune system

decreased osteoclast cell number ( J:116609 )

• reduced formation of tartrate resistant alkaline phosphatase + (TRAP+) osteoclasts after stimulation with M-CSF/RANKL

• reduced number of TRAP+ ostoclasts in femoral bones of 18 week old mice, by 29%

• 37% reduction in osteoclast surface in the distal femoral metaphysis

abnormal neutrophil physiology ( J:36489 )

• in vitro, bone marrow-derived homozygous mutant PMNs do not bind particles coated with the PMN beta3 integrin ligand peptide KGAGDVA (K,Lys; G, Gly; A, Ala; D, Asp; V, Val) and fail to generate an oxidative burst in response to surface-bound peptide

• in contrast, binding of C3bi-opsonized particles by homozygous mutant PMNs is normal

• the effector response of homozygous mutant PMNs to control stimuli phorbol dibutyrate and fMLP is also normal

impaired neutrophil chemotaxis ( J:36489 )

• at 4 hrs after i.p. challenge with 3 x 10⁴ virulent E. coli (018:K1:H7), homozygotes display a reduced influx of both PMNs and other cells (mainly mononuclear leukocytes) relative to heterozygous littermates

• at 24 hrs after i.p. challenge with 4.3 x 10⁴ virulent E. coli (018:K1:H7), the % of PMNs in peritoneal lavages of homozygous mutant mice is reduced relative to that in heterozygous controls

impaired neutrophil phagocytosis ( J:36489 )

• upon stimulation with eight-KGAGDVA-valent peptide (AGD, 20 ug/ml), bone marrow-derived homozygous mutant PMNs exhibit a significant reduction in IgG Fc receptor-mediated phagocytosis relative to wild-type or heterozygous PMNs

• higher AGD concentrations (30-40 ug/ml) not only fail to activate but actually inhibit IgG Fc receptor-mediated phagocytosis by homozygous mutant PMNs

impaired granulocyte bactericidal activity ( J:36489 )

• in vitro, purified PMNs from homozygous mutant mice display significant defects in several aspects of the antibacterial neutrophil activation process that lead to E. coli killing and effective host defense

increased susceptibility to bacterial infection ( J:36489 )

• at 24 hrs after i.p. challenge with 4.3 x 10⁴ virulent E. coli (018:K1:H7), >100-fold more E. coli are recovered from the peritoneal cavities and spleens of homozygous mutant mice than from heterozygote controls, suggesting a reduced ability to control infection

• at 24 hrs after E. coli challenge, all homozygotes display more signs of severe infection than control littermates, including hunched posture, ruffled fur, hypothermia, impaired ambulation, and lethargy

increased susceptibility to bacterial infection induced morbidity/mortality ( J:36489 )

• following i.p. injection of 5 x 10⁴ virulent E.coli (018:K1:H7), all 8-week-old homozygotes die at 2 days after challenge, whereas all heterozygous littermates survive the infection

hematopoietic system

thrombocytopenia ( J:98979 )

• mild thrombocytopenia

decreased osteoclast cell number ( J:116609 )

• reduced formation of tartrate resistant alkaline phosphatase + (TRAP+) osteoclasts after stimulation with M-CSF/RANKL

• reduced number of TRAP+ ostoclasts in femoral bones of 18 week old mice, by 29%

• 37% reduction in osteoclast surface in the distal femoral metaphysis

abnormal neutrophil physiology ( J:36489 )

• in contrast, binding of C3bi-opsonized particles by homozygous mutant PMNs is normal

• the effector response of homozygous mutant PMNs to control stimuli phorbol dibutyrate and fMLP is also normal

impaired neutrophil chemotaxis ( J:36489 )

• at 24 hrs after i.p. challenge with 4.3 x 10⁴ virulent E. coli (018:K1:H7), the % of PMNs in peritoneal lavages of homozygous mutant mice is reduced relative to that in heterozygous controls

impaired neutrophil phagocytosis ( J:36489 )

• higher AGD concentrations (30-40 ug/ml) not only fail to activate but actually inhibit IgG Fc receptor-mediated phagocytosis by homozygous mutant PMNs

impaired granulocyte bactericidal activity ( J:36489 )

abnormal platelet physiology ( J:98979 )

• platelets show a considerably shorter half life when injected in wild-type recipients

skeleton

decreased osteoclast cell number ( J:116609 )

• reduced formation of tartrate resistant alkaline phosphatase + (TRAP+) osteoclasts after stimulation with M-CSF/RANKL

• reduced number of TRAP+ ostoclasts in femoral bones of 18 week old mice, by 29%

• 37% reduction in osteoclast surface in the distal femoral metaphysis

homeostasis/metabolism

abnormal platelet physiology ( J:98979 )

• platelets show a considerably shorter half life when injected in wild-type recipients

behavior/neurological

behavioral despair ( J:162849 )

• increased immobility times on second day of forced swimming test

cellular

impaired neutrophil chemotaxis ( J:36489 )

• at 24 hrs after i.p. challenge with 4.3 x 10⁴ virulent E. coli (018:K1:H7), the % of PMNs in peritoneal lavages of homozygous mutant mice is reduced relative to that in heterozygous controls

impaired neutrophil phagocytosis ( J:36489 )

• higher AGD concentrations (30-40 ug/ml) not only fail to activate but actually inhibit IgG Fc receptor-mediated phagocytosis by homozygous mutant PMNs

Allelic Composition	Cd47^tm1Fpl/Cd47^tm1Fpl
Genetic Background	C.129S7-Cd47^tm1Fpl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd47^tm1Fpl mutation (4 available); any Cd47 mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

abnormal passive avoidance behavior ( J:59818 )

• reduced retention 3 hours after inhibitory avoidance training

nervous system

abnormal excitatory postsynaptic potential ( J:59818 )

• significantly reduced slope of population excitatory postsynaptic potential upon tetanic stimulation

• population spike amplitudes are lower

hematopoietic system

thrombocytopenia ( J:98979 )

• mild thrombocytopenia

decreased osteoclast cell number ( J:116609 )

• reduced formation of tartrate resistant alkaline phosphatase + (TRAP+) osteoclasts after stimulation with M-CSF/RANKL

• reduced number of TRAP+ ostoclasts in femoral bones of 18 week old mice, by 29%

• 37% reduction in osteoclast surface in the distal femoral metaphysis

abnormal platelet physiology ( J:98979 )

• platelets show a considerably shorter half life when injected in wild-type recipients

skeleton

decreased osteoclast cell number ( J:116609 )

• reduced formation of tartrate resistant alkaline phosphatase + (TRAP+) osteoclasts after stimulation with M-CSF/RANKL

• reduced number of TRAP+ ostoclasts in femoral bones of 18 week old mice, by 29%

• 37% reduction in osteoclast surface in the distal femoral metaphysis

vision/eye

vision/eye phenotype ( J:107935 )

N	• electroretinograms are normal

homeostasis/metabolism

abnormal platelet physiology ( J:98979 )

• platelets show a considerably shorter half life when injected in wild-type recipients

immune system

decreased osteoclast cell number ( J:116609 )

• reduced formation of tartrate resistant alkaline phosphatase + (TRAP+) osteoclasts after stimulation with M-CSF/RANKL

• reduced number of TRAP+ ostoclasts in femoral bones of 18 week old mice, by 29%

• 37% reduction in osteoclast surface in the distal femoral metaphysis

Allelic Composition	Cd47^tm1Fpl/Cd47^tm1Fpl
Genetic Background	involves: 129S7/SvEvBrd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd47^tm1Fpl mutation (4 available); any Cd47 mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased T cell number ( J:36489 )

• homozygotes display a reduction in the CD3⁺ fraction of peripheral lymphocytes relative to control littermates

• however, no significant differences in platelet, PMN or WBC count are observed relative to wild-type or heterozygous controls at all ages tested

impaired neutrophil recruitment ( J:148196 )

• neutrophil infiltration into ischemic regions is considerably reduced

nervous system

abnormal axon morphology ( J:116188 )

• fewer hippocampal neurons with an enlarged growth cone at the end of the primary axon

abnormal dendrite morphology ( J:116188 )

• hippocampal neurons in culture develop fewer dendrites than do controls after 3-7 days

• reduced branching of dendrites at 3-7 but not at 14-21 days

• total length of dendrites is markedly reduced at 3 and 7 days in culture

brain ischemia ( J:148196 )

• ischemic swelling after occlusion/reperfusion in the brain is similar to controls at 24 hours but does not undergo further development as it does in controls

• infarct volume, corrected for hemispheric swelling is considerably smaller than in controls at both 24 and 72 hours

• neutrophil infiltration into ischemic regions is considerably reduced

integument

integument phenotype ( J:133700 )

N	• normal cutaneous architecture is maintained in myocutaneous skin flap preparations

abnormal skin physiology ( J:133700 )

• improved myocutaneous skin flap survival 7 days after the surgery as compared to controls

• reduced necrosis

limbs/digits/tail

abnormal hindlimb morphology ( J:133700 )

• better tissue survival after proximal ligation of the external iliac and common femoral

• minimal or no clinical evidence of tissue necrosis

• increased vascular remodeling and vascular perfusion by 1 week after the operation

cardiovascular system

abnormal physiological neovascularization ( J:133700 )

• increased vascular remodeling and vascular perfusion by proximal ligation of the external iliac and common femoral

cellular

abnormal mitochondrial physiology ( J:133700 )

• mitochondrial function is maintained better 1 week after proximal ligation of the external iliac and common femoral artery than in controls

immune system

decreased T cell number ( J:36489 )

• homozygotes display a reduction in the CD3⁺ fraction of peripheral lymphocytes relative to control littermates

• however, no significant differences in platelet, PMN or WBC count are observed relative to wild-type or heterozygous controls at all ages tested

impaired neutrophil recruitment ( J:148196 )

• neutrophil infiltration into ischemic regions is considerably reduced

Allelic Composition	Cd47^tm1Fpl/Cd47^tm1Fpl
Genetic Background	involves: 129S7/SvEvBrd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd47^tm1Fpl mutation (4 available); any Cd47 mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

respiratory system

pleural effusion ( J:134866 )

• accumulation of excess fluid in the lungs 24 hours after intratracheal instillation with LPS or after lung infection with E. coli is reduced

• bronchoalveolar lavage protein concentrations reduced

hematopoietic system

abnormal osteoclast differentiation ( J:147367 )

• macrophage differentiation into osteoclasts is severely reduced in culture

• increased doses of RANKL increases osteoclast formation

decreased hematocrit ( J:134866 )

• relative to controls after intratracheal instillation with LPS or after lung infection with E. coli

decreased hemoglobin content ( J:134866 )

• relative to controls after intratracheal instillation with LPS or after lung infection with E. coli

decreased leukocyte cell number ( J:134866 )

• significantly reduced relative to controls in both blood and bronchoalveolar lavage after intratracheal instillation with LPS

decreased neutrophil cell number ( J:134866 )

• significantly reduced relative to controls in both blood and bronchoalveolar lavage after intratracheal instillation with LPS or after lung infection with E. coli

skeleton

abnormal bone structure ( J:147367 )

abnormal osteoclast differentiation ( J:147367 )

• macrophage differentiation into osteoclasts is severely reduced in culture

• increased doses of RANKL increases osteoclast formation

increased trabecular bone volume ( J:147367 )

• by microCT analysis

increased trabecular bone thickness ( J:147367 )

• thickness but not number is increased in tibias

abnormal skeleton physiology ( J:147367 )

• reduced cleavage of type I collagen

decreased bone resorption ( J:147367 )

• osteoclast bone resorption is reduced unless RANKL concentration is elevated

cardiovascular system

increased heart rate ( J:148948 )

• in active light cycle

increased mean systemic arterial blood pressure ( J:148948 )

• increased 15% relative to controls under isofluorane anaesthesia

increased systemic arterial diastolic blood pressure ( J:148948 )

• in active light cycle

• peripheral diastolic pressure is significantly increased

increased systemic arterial systolic blood pressure ( J:148948 )

• peripheral systolic blood pressure is significantly increased

integument

decreased sensitivity to skin irradiation ( J:139652 )

• hair is preserved after 25 Gy irradiation

• minimal or no skin ulceration 8 weeks after irradiation

• hair follicles and skin architecture are better preserved than in controls

muscle

muscle phenotype ( J:139652 )

N	• no leg muscle atrophy after irradiation • limb flexibility maintained

abnormal muscle fiber morphology ( J:139652 )

• less loss of muscle fibers and nuclei after irradiation

homeostasis/metabolism

pleural effusion ( J:134866 )

• accumulation of excess fluid in the lungs 24 hours after intratracheal instillation with LPS or after lung infection with E. coli is reduced

• bronchoalveolar lavage protein concentrations reduced

abnormal cytokine level ( J:134866 )

• MIP-2 levels in bronchoalveolar fluid are reduced 4 hours after LPS challenge but not after 24 hours

abnormal tumor necrosis factor level ( J:134866 )

• levels in bronchoalveolar fluid are reduced 4 hours after LPS challenge but not after 24 hours

immune system

abnormal osteoclast differentiation ( J:147367 )

• macrophage differentiation into osteoclasts is severely reduced in culture

• increased doses of RANKL increases osteoclast formation

decreased leukocyte cell number ( J:134866 )

• significantly reduced relative to controls in both blood and bronchoalveolar lavage after intratracheal instillation with LPS

decreased neutrophil cell number ( J:134866 )

• significantly reduced relative to controls in both blood and bronchoalveolar lavage after intratracheal instillation with LPS or after lung infection with E. coli

abnormal cytokine level ( J:134866 )

• MIP-2 levels in bronchoalveolar fluid are reduced 4 hours after LPS challenge but not after 24 hours

abnormal tumor necrosis factor level ( J:134866 )

• levels in bronchoalveolar fluid are reduced 4 hours after LPS challenge but not after 24 hours

decreased susceptibility to bacterial infection induced morbidity/mortality ( J:134866 )

• enhanced survival 72 hours after E. coli infection with 5 x 10 ⁶ CFU

increased susceptibility to bacterial infection ( J:134866 )

• after E coli infection of the lungs, bacterial levels remain high in the lungs but are reduced in the blood

neoplasm

decreased tumor incidence ( J:147367 )

• fewer tumors develop in femur, tibia and mandible from B16-F10 melanoma cell injection

mortality/aging

decreased susceptibility to bacterial infection induced morbidity/mortality ( J:134866 )

• enhanced survival 72 hours after E. coli infection with 5 x 10 ⁶ CFU

cellular

abnormal osteoclast differentiation ( J:147367 )

• macrophage differentiation into osteoclasts is severely reduced in culture

• increased doses of RANKL increases osteoclast formation

nervous system

nervous system phenotype ( J:204011 )

N	• exhibit normal neurite differentiation

Allelic Composition	Cd47^tm1Fpl/Cd47^tm1Fpl
Genetic Background	NOD.129S7-Cd47^tm1Fpl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd47^tm1Fpl mutation (4 available); any Cd47 mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:76580 )

• death between 300 and 550 days from severe hemolytic anemia

hematopoietic system

abnormal erythropoiesis ( J:76580 )

• expanded erythroid cell numbers in vertebrae, sternum, rib tips and long bone marrow cavities

hemolytic anemia ( J:76580 )

• severe and rapidly fatal hemolytic anemia develops between 180 and 280 days of age whereas NOD controls develop only a mildly hemolytic anemia at 300-550 days

• anemia is secondary to rapid destruction of RBC

• high levels of IgG and IgM on RBC of anemic mice

increased bone marrow cell number ( J:76580 )

• expanded erythroid cell numbers in long bone marrow cavities

decreased hematocrit ( J:76580 )

• hematocrit at 194 days is 33% of controls

reticulocytosis ( J:76580 )

• at 194 days

abnormal spleen morphology ( J:76580 )

enlarged spleen ( J:76580 )

• massively enlarged spleen at death, 10X normal size

abnormal splenic cell ratio ( J:76580 )

• majority of spleen cels are erythropoietic

immune system

abnormal spleen morphology ( J:76580 )

enlarged spleen ( J:76580 )

• massively enlarged spleen at death, 10X normal size

abnormal splenic cell ratio ( J:76580 )

• majority of spleen cels are erythropoietic

growth/size/body

enlarged spleen ( J:76580 )

• massively enlarged spleen at death, 10X normal size

Allelic Composition	Cd47^tm1Fpl/Cd47⁺
Genetic Background	B6.129S7-Cd47^tm1Fpl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd47^tm1Fpl mutation (4 available); any Cd47 mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality ( J:36489 )

• whereas all 8-week-old heterozygotes survive after i.p. injection of 5 x 10⁴ virulent E.coli (018:K1:H7), some heterozygotes succumb to infection when a 10-fold higher inoculum is used

immune system

increased susceptibility to bacterial infection induced morbidity/mortality ( J:36489 )

• whereas all 8-week-old heterozygotes survive after i.p. injection of 5 x 10⁴ virulent E.coli (018:K1:H7), some heterozygotes succumb to infection when a 10-fold higher inoculum is used

Allelic Composition	Rag2^tm1Fwa/Rag2^tm1Fwa Cd47^tm1Fpl/Cd47^tm1Fpl Il2rg^tm1Cgn/Y
Genetic Background	involves: 129P2/OlaHsd * 129S/SvEv * 129S7/SvEvBrd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd47^tm1Fpl mutation (4 available); any Cd47 mutation (48 available)
Il2rg^tm1Cgn mutation (4 available); any Il2rg mutation (179 available)
Rag2^tm1Fwa mutation (45 available); any Rag2 mutation (119 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased CD8-positive, alpha-beta T cell number ( J:203904 )

• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells

increased activated T cell number ( J:203904 )

• a high proportion of CD38+ CD8+ T cells appear activated in HIV-infected BLT transplanted mice

decreased lymphocyte cell number ( J:203904 )

• spleen and lymph nodes lack lymphocytes

decreased CD4-positive, alpha-beta T cell number ( J:203904 )

• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells

absent peripheral lymph nodes ( J:203904 )

• mice fail to develop peripheral lymph nodes

increased susceptibility to Retroviridae infection ( J:203904 )

• 54% of BLT transplanted mice inoculated intrarectally with HIV become infected with plasma viral titers peaking between 2 and 4 weeks

• 100% of transplanted mice inoculated intraperitoneally with HIV become infected

• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells

• a high proportion of CD8+ T cells appear activated in HIV-infected BLT transplanted mice

• infected mice produce HIV-specific antibodies and can mount HIV-specific T cell responses

decreased susceptibility to graft versus host disease ( J:203904 )

• mice are tolerized to xenotransplantation with human bone marrow, liver, thymus (BLT)

• mice average 1x10⁶ human leukocytes/mL blood 12 weeks after transplantation and levels remain stable or improve through 21 weeks

• >80% of leukocytes in peripheral circulation are human

• transplanted mice have higher levels of multipotent bone marrow/hematopoietic stem cells than mice heterozygous for the Cd47^tm1Fpl allele

• GVHD is not evident in mice 25 weeks post-transplantation

• human thymic organoids are highly vascularized

• large and small intestines of transplanted mice contain CD4+ and CD8+ human T cells

hematopoietic system

increased CD8-positive, alpha-beta T cell number ( J:203904 )

• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells

increased activated T cell number ( J:203904 )

• a high proportion of CD38+ CD8+ T cells appear activated in HIV-infected BLT transplanted mice

decreased lymphocyte cell number ( J:203904 )

• spleen and lymph nodes lack lymphocytes

decreased CD4-positive, alpha-beta T cell number ( J:203904 )

• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells

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