Phenotypes associated with this allele

• Allele Symbol: Pecam1^{Gt(OST16303)Lex}
• Allele Name: gene trap OST16303, Lexicon Genetics
• Allele ID: MGI:1888376
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pecam1^Gt(OST16303)Lex/Pecam1^Gt(OST16303)Lex	B6.129S-Pecam1^Gt(OST16303)Lex/J	MGI:3785248
hm2	Pecam1^Gt(OST16303)Lex/Pecam1^Gt(OST16303)Lex	B6.129S5-Pecam1^Gt(OST16303)Lex	MGI:3785247
cx3	Apoe^tm1Unc/Apoe^tm1Unc Pecam1^Gt(OST16303)Lex/Pecam1^Gt(OST16303)Lex	B6.129-Apoe^tm1Unc Pecam1^Gt(OST16303)Lex	MGI:3822293

Genotype
MGI:3785248

hm1

• Allelic Composition: Pecam1^{Gt(OST16303)Lex}/Pecam1^{Gt(OST16303)Lex}
• Genetic Background: B6.129S-Pecam1^{Gt(OST16303)Lex}/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal vasodilation ( J:114343 )

• unlike in wild-type mice, vessel dilation is not reduced by L-NAME

• however, treatment with A23187 (an agonist that stimulates Nos3) restores sensitivity to L-NAME

decreased vasodilation ( J:114343 )

• flow mediated dilation of arterioles is reduced compared to in wild-type mice

muscle

abnormal vasodilation ( J:114343 )

• unlike in wild-type mice, vessel dilation is not reduced by L-NAME

• however, treatment with A23187 (an agonist that stimulates Nos3) restores sensitivity to L-NAME

decreased vasodilation ( J:114343 )

• flow mediated dilation of arterioles is reduced compared to in wild-type mice

Genotype
MGI:3785247

hm2

• Allelic Composition: Pecam1^{Gt(OST16303)Lex}/Pecam1^{Gt(OST16303)Lex}
• Genetic Background: B6.129S5-Pecam1^{Gt(OST16303)Lex}

cardiovascular system

decreased vasodilation ( J:104082 )

• flow mediated dilation of the coronary artery is reduced compared to in wild-type mice

• a slightly greater proportion of dilation is sensitive to idomethacin compared to in wild-type mice

• mice are more sensitive to inhibition of endothelium-derived hyperpolarizing factor associated dilation than wild-type mice

• mice lack dilation sensitivity to peroxide unlike wild-type mice

• however, normal flow mediated dilation can be restored with treatment with scavengers for ONOO-

homeostasis/metabolism

abnormal nitric oxide homeostasis ( J:104082 )

• nitric oxide production in the coronary artery is increased compared to in wild-type mice

muscle

decreased vasodilation ( J:104082 )

• flow mediated dilation of the coronary artery is reduced compared to in wild-type mice

• a slightly greater proportion of dilation is sensitive to idomethacin compared to in wild-type mice

• mice are more sensitive to inhibition of endothelium-derived hyperpolarizing factor associated dilation than wild-type mice

• mice lack dilation sensitivity to peroxide unlike wild-type mice

• however, normal flow mediated dilation can be restored with treatment with scavengers for ONOO-

Genotype
MGI:3822293

cx3

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Pecam1^{Gt(OST16303)Lex}/Pecam1^{Gt(OST16303)Lex}
• Genetic Background: B6.129-Apoe^tm1Unc Pecam1^{Gt(OST16303)Lex}

cardiovascular system

abnormal aorta endothelium morphology ( J:142083 )

• Cx43 distribution at cell-cell junction shows less disruption than in single Apoe-deficient mice

atherosclerotic lesions ( J:142083 )

• major lesions are observed on lesser curvature of aortic arch in males and females with minor lesions found at branches of aortic arch

• lesions comprise around 14-16% of total aortic arch area in male and female mutants

• aortic lesions are observed in aortic sinus at level of aortic valve leaflets; lesions are about 27% of total aortal area

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:142083 )

N • cholesterol levels of double mutants on a Western or normal chow diet are not significantly different from diet-matched Apoe-null animals