Phenotypes associated with this allele

• Allele Symbol: Tcf7l2^tm1Cle
• Allele Name: targeted mutation 1, Hans Clevers
• Allele ID: MGI:1926344
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tcf7l2^tm1Cle/Tcf7l2^tm1Cle	involves: 129P2/OlaHsd	MGI:3032499
cn2	Tcf7l1^tm1.1Efu/Tcf7l1^tm1.1Efu Tcf7l2^tm1Cle/Tcf7l2^tm1Cle Tg(KRT14-cre)1Efu/0	involves: 129/Sv * 129P2/OlaHsd	MGI:4413471

Genotype
MGI:3032499

hm1

• Allelic Composition: Tcf7l2^tm1Cle/Tcf7l2^tm1Cle
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:48968 )

• pups die within 24 hours of birth

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:48968 )

• at E16.5 there are no cycling cells detected in intestinal epithelium

abnormal enterocyte morphology ( J:48968 )

• cells of intervillus region do not have characteristics of intervillus prospective crypt cells, instead displaying characteristics including closely packed long microvilli, round nuclei, supranuclear Golgi, many rod-shaped mitochondria, and absence of mitotic figures

decreased enterocyte cell number ( J:48968 )

• enterocytes on villi are decreased in number and broader in homozygotes than in wild-type

endocrine/exocrine glands

enlarged adenohypophysis ( J:86317 )

• anterior pituitary lobes are enlarged to nearly twice the normal height by E14.5

enlarged pituitary gland ( J:86317 )

• a 3-fold increase in combined volume of anterior and intermediate lobes is observed compared to wild-type mice

abnormal pituitary intermediate lobe morphology ( J:86317 )

• prospective intermediate lobe and infundibulum are displaced dorsally; these protrude into space normally occupied by hypothalamus

craniofacial

abnormal craniofacial development ( J:86317 )

• pituitary gland is expanded ventrally between edges of cartilage plate in the midline during palate development

cellular

cellular phenotype ( J:98680 )

N • no differences in cell proliferation in tissue of small intestine is observed at E14.5

nervous system

enlarged adenohypophysis ( J:86317 )

• anterior pituitary lobes are enlarged to nearly twice the normal height by E14.5

enlarged pituitary gland ( J:86317 )

• a 3-fold increase in combined volume of anterior and intermediate lobes is observed compared to wild-type mice

abnormal pituitary intermediate lobe morphology ( J:86317 )

• prospective intermediate lobe and infundibulum are displaced dorsally; these protrude into space normally occupied by hypothalamus

Genotype
MGI:4413471

cn2

• Allelic Composition: Tcf7l1^tm1.1Efu/Tcf7l1^tm1.1Efu; Tcf7l2^tm1Cle/Tcf7l2^tm1Cle; Tg(KRT14-cre)1Efu/0
• Genetic Background: involves: 129/Sv * 129P2/OlaHsd

integument

abnormal hair follicle morphology ( J:155012 )

• hair follicles appear but exhibit defects in subsequent down-growth compared to in wild-type mice

absent hair follicles ( J:155012 )

• in skin grafts

absent vibrissae ( J:155012 )

• newborn mice often lack whiskers unlike wild-type mice

abnormal epidermis stratum basale morphology ( J:155012 )

• basal cells are flattened unlike in wild-type mice

thin epidermis ( J:155012 )

thin skin ( J:155012 )

• in newborn mice

abnormal skin physiology ( J:155012 )

• at P0, skin cells exhibit increased cell death compared to in wild-type mice

• grafted skin exhibits no hair and shrunk area without inflammation compared with wild-type skin grafts

• cultured skin epithelial fails to undergo long-term tissue maintenance unlike wild-type skin

decreased keratinocyte proliferation ( J:155012 )

• cultured keratinocytes from P0 mice exhibit decreased proliferation compared with wild-type cells

cellular

decreased keratinocyte proliferation ( J:155012 )

• cultured keratinocytes from P0 mice exhibit decreased proliferation compared with wild-type cells