All Phenotypes Pax5<tm1Mbu> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pax5^tm1Mbu/Pax5^tm1Mbu	either: 129 or (involves: 129S2/SvPas * C57BL/6J)	MGI:5515635
hm2	Pax5^tm1Mbu/Pax5^tm1Mbu	involves: 129S2/SvPas	MGI:3720350
hm3	Pax5^tm1Mbu/Pax5^tm1Mbu	involves: 129S2/SvPas * C57BL/6	MGI:3665143
ht4	Pax5^tm1Mbu/Pax5⁺	involves: 129S2/SvPas * C57BL/6	MGI:2654625
cn5	Cd19^tm1(cre)Cgn/Cd19⁺ Pax5^tm1Mbu/Pax5^tm3Mbu	involves: 129P2/OlaHsd * 129S2/SvPas	MGI:3720352
cn6	Pax5^tm1Mbu/Pax5^tm3Mbu Tg(Mx1-cre)1Cgn/?	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * CBA	MGI:3720351
cx7	Igh^tm1(PAX5)Mbu/Igh⁺ Pax5^tm1Mbu/Pax5^tm1Mbu	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * CBA	MGI:3701078

Allelic Composition	Pax5^tm1Mbu/Pax5^tm1Mbu
Genetic Background	either: 129 or (involves: 129S2/SvPas * C57BL/6J)

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5^tm1Mbu mutation (1 available); any Pax5 mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal lymphopoiesis ( J:21935 )

• no pre-B cell lines can be obtained from Pax5-deficient livers while they are readily established from livers of fetal heterozygous and wild-type livers

• fetal liver cannot support development of early B-lymphoid progenitors

• when fetal liver cells from mutants are transplanted into lethally irradiated hosts however, pre-B cell precursors can be detected in bone marrow of hosts

abnormal late pro-B cell ( J:21935 )

• incidence of V to DJ rearrangement of the immunoglobulin heavy chain is reduced 10-fold in Pax5-deficient mice

absent late pro-B cells ( J:21935 )

• development is halted at early pro-B cell stage

arrested B cell differentiation ( J:21935 )

• B cell development is arrested at the early pro-B stage in bone marrow

immune system

abnormal lymphopoiesis ( J:21935 )

• no pre-B cell lines can be obtained from Pax5-deficient livers while they are readily established from livers of fetal heterozygous and wild-type livers

• fetal liver cannot support development of early B-lymphoid progenitors

• when fetal liver cells from mutants are transplanted into lethally irradiated hosts however, pre-B cell precursors can be detected in bone marrow of hosts

abnormal late pro-B cell ( J:21935 )

• incidence of V to DJ rearrangement of the immunoglobulin heavy chain is reduced 10-fold in Pax5-deficient mice

absent late pro-B cells ( J:21935 )

• development is halted at early pro-B cell stage

arrested B cell differentiation ( J:21935 )

• B cell development is arrested at the early pro-B stage in bone marrow

Allelic Composition	Pax5^tm1Mbu/Pax5^tm1Mbu
Genetic Background	involves: 129S2/SvPas

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5^tm1Mbu mutation (1 available); any Pax5 mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

lethality at weaning, complete penetrance ( J:75504 )

• mice die at weaning

immune system

abnormal immunoglobulin heavy chain V(D)J recombination ( J:139510 )

arrested B cell differentiation ( J:75504 )

• B cell development is arrested in the pro-B cell stage

abnormal B cell physiology ( J:123629 )

• in culture pro-B cells exhibit 32 times poorer adhesion to a VCAM-1 coated surface compared to wild-type cells

enhanced B cell migration ( J:123629 )

• in a transwell migration assay, pro-B cells have a 4-fold increase in migration towards a source of CXCL12 compared to wild-type mice

growth/size/body

postnatal growth retardation ( J:75504 )

cellular

abnormal cell adhesion ( J:123629 )

• in culture pro-B cells exhibit 32 times poorer adhesion to a VCAM-1 coated surface compared to wild-type cells

enhanced B cell migration ( J:123629 )

• in a transwell migration assay, pro-B cells have a 4-fold increase in migration towards a source of CXCL12 compared to wild-type mice

hematopoietic system

abnormal immunoglobulin heavy chain V(D)J recombination ( J:139510 )

arrested B cell differentiation ( J:75504 )

• B cell development is arrested in the pro-B cell stage

abnormal B cell physiology ( J:123629 )

• in culture pro-B cells exhibit 32 times poorer adhesion to a VCAM-1 coated surface compared to wild-type cells

enhanced B cell migration ( J:123629 )

• in a transwell migration assay, pro-B cells have a 4-fold increase in migration towards a source of CXCL12 compared to wild-type mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:113076 )

• only 11/200 animals survive past weaning and live up to 7 months

postnatal lethality, incomplete penetrance ( J:113076 )

• homozygotes usually die in third week after birth

growth/size/body

decreased body size ( J:113076 )

• mice show severe growth retardation in the second week after birth; mice that survive through weaning are severely runted

decreased body weight ( J:113076 )

• at time of death, weight is ~1/3 weight of littermates

behavior/neurological

limb grasping ( J:113076 )

• mice consistently flex both hindlimbs and sometimes their forelimbs upon tail suspension, while wild-type mice extend their legs

• behavior is pronounced in young animals 2-3 weeks of age, but becomes attenuated in adults which usually clasp only 1 hindleg

nervous system

abnormal hindbrain development ( J:113076 )

• at E16.5 posterior region of the collicular neuroepithelium is detectably shorter and thinner vs wild-type

abnormal cerebellar foliation ( J:113076 )

• mice show altered cerebellar foliation pattern

• lobule culmen is divided by a deep intraculminate fissure

decreased inferior colliculus size ( J:113076 )

• structure is dramatically reduced in central region around midline while appearing normal on lateral sides

• however, superior colliculus is of normal size

abnormal cerebellum morphology ( J:113076 )

• culmen of cerebellum is expanded anteriorly

abnormal cerebellum vermis morphology ( J:113076 )

• minor change in folding of tuber vermis is observed

immune system

abnormal lymphopoiesis ( J:38813 )

• fetal liver does not support B cell lymphopoiesis; wild-type and heterozygous livers contain only 3-4% B lymphocytes in total population but mutants have no B lymphocytes at E17

abnormal B cell differentiation ( J:38813 )

• incidence of V to DJ heavy chain rearrangements is 50- to 70-fold reduced in mutant pre-BI cells compared to wild-type; D to J rearrangements are not affected

decreased immature B cell number ( J:113076 )

• at 2 weeks of age, numbers of immature B cells in spleen are considerably reduced; similar results are found in bone marrow and lymph nodes

arrested B cell differentiation ( J:113076 )

• B cell development is stopped at early stage; B cells are large and express CD43 and B220 on the surface consistent with early precursor stage

decreased B cell number ( J:113076 )

absent mature B cells ( J:113076 )

• mutants lack mature B cells

decreased B-1 B cell number ( J:113076 )

• at 2 weeks, peritoneum is devoid of conventional B cells and mature B-1 cells

absent B-2 B cells ( J:113076 )

• at 2 weeks, peritoneum is devoid of conventional B cells

increased T cell number ( J:113076 )

• mature T cells are present in spleen

• number of mature T cells appears increased 2- to 4-fold as result of deletion of mature B cells

decreased immunoglobulin level ( J:113076 )

• no immunoglobulin is detected above detection limit compared to normal concentrations in wild-type and heterozygotes

hematopoietic system

abnormal lymphopoiesis ( J:38813 )

• fetal liver does not support B cell lymphopoiesis; wild-type and heterozygous livers contain only 3-4% B lymphocytes in total population but mutants have no B lymphocytes at E17

abnormal B cell differentiation ( J:38813 )

• incidence of V to DJ heavy chain rearrangements is 50- to 70-fold reduced in mutant pre-BI cells compared to wild-type; D to J rearrangements are not affected

decreased immature B cell number ( J:113076 )

• at 2 weeks of age, numbers of immature B cells in spleen are considerably reduced; similar results are found in bone marrow and lymph nodes

arrested B cell differentiation ( J:113076 )

• B cell development is stopped at early stage; B cells are large and express CD43 and B220 on the surface consistent with early precursor stage

decreased B cell number ( J:113076 )

absent mature B cells ( J:113076 )

• mutants lack mature B cells

decreased B-1 B cell number ( J:113076 )

• at 2 weeks, peritoneum is devoid of conventional B cells and mature B-1 cells

absent B-2 B cells ( J:113076 )

• at 2 weeks, peritoneum is devoid of conventional B cells

increased T cell number ( J:113076 )

• mature T cells are present in spleen

• number of mature T cells appears increased 2- to 4-fold as result of deletion of mature B cells

decreased immunoglobulin level ( J:113076 )

• no immunoglobulin is detected above detection limit compared to normal concentrations in wild-type and heterozygotes

hearing/vestibular/ear

hearing/vestibular/ear phenotype ( J:113076 )

• at 13-19 days of age, homozygotes exhibit normal auditory responses and audiograms relative to wild-type mice, suggesting that the affected central region of the inferior colliculus is not important for hearing, at least as assessed by early brainstem auditory evoked potential (BAEP) measurements

• however, development of auditory sensitivity is delayed by at least 1 day, and peak latencies of BAEPs for waves II and IV are significantly longer, consistent with a general growth retardation

Allelic Composition	Pax5^tm1Mbu/Pax5⁺
Genetic Background	involves: 129S2/SvPas * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5^tm1Mbu mutation (1 available); any Pax5 mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal cerebellum morphology ( J:113076 )

• culmen of cerebellum is slightly expanded anteriorly, but inferior colliculus appears normal as opposed to homozygotes

abnormal cerebellar lobule formation ( J:113076 )

• only shallow intraculminate fissure is observed compared to homozygotes

Allelic Composition	Cd19^tm1(cre)Cgn/Cd19⁺ Pax5^tm1Mbu/Pax5^tm3Mbu
Genetic Background	involves: 129P2/OlaHsd * 129S2/SvPas

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19^tm1(cre)Cgn mutation (11 available); any Cd19 mutation (60 available)
Pax5^tm1Mbu mutation (1 available); any Pax5 mutation (37 available)
Pax5^tm3Mbu mutation (1 available); any Pax5 mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell differentiation ( J:75504 )

• LPS-stimulated lymph node B cells exhibit a low frequency of and a delay in increased cell size relative to wild-type cells

abnormal class switch recombination ( J:75504 )

• IgG class switching is decreased

decreased immature B cell number ( J:75504 )

• short-lived IgM-B220^lo and IgM+B220^lo immature B cells are slightly lower in number

decreased mature B cell number ( J:75504 )

• there is a 3-fold reduction in the number of long-lived mature B cells

• mice lack recirculating IgM+IgD+B220^hi cells in the bone marrow

decreased pre-B cell number ( J:75504 )

• short-lived IgM-B220^lo B cells are slightly lower in number

decreased immunoglobulin level ( J:75504 )

• total immunoglobin is reduced 3-fold compared to control mice

decreased IgG level ( J:75504 )

• IgG levels are decreased ranging from a 9.4-fold decrease of IgG1 to a 2-fold decrease in IgG3 levels

decreased IgG1 level ( J:75504 )

decreased IgG3 level ( J:75504 )

hematopoietic system

abnormal B cell differentiation ( J:75504 )

• LPS-stimulated lymph node B cells exhibit a low frequency of and a delay in increased cell size relative to wild-type cells

abnormal class switch recombination ( J:75504 )

• IgG class switching is decreased

decreased immature B cell number ( J:75504 )

• short-lived IgM-B220^lo and IgM+B220^lo immature B cells are slightly lower in number

decreased mature B cell number ( J:75504 )

• there is a 3-fold reduction in the number of long-lived mature B cells

• mice lack recirculating IgM+IgD+B220^hi cells in the bone marrow

decreased pre-B cell number ( J:75504 )

• short-lived IgM-B220^lo B cells are slightly lower in number

decreased immunoglobulin level ( J:75504 )

• total immunoglobin is reduced 3-fold compared to control mice

decreased IgG level ( J:75504 )

• IgG levels are decreased ranging from a 9.4-fold decrease of IgG1 to a 2-fold decrease in IgG3 levels

decreased IgG1 level ( J:75504 )

decreased IgG3 level ( J:75504 )

Allelic Composition	Pax5^tm1Mbu/Pax5^tm3Mbu Tg(Mx1-cre)1Cgn/?
Genetic Background	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5^tm1Mbu mutation (1 available); any Pax5 mutation (37 available)
Pax5^tm3Mbu mutation (1 available); any Pax5 mutation (37 available)
Tg(Mx1-cre)1Cgn mutation (7 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased mature B cell number ( J:75504 )

• half of the mature B cells in the spleen are absent

• only a small number of mature B cells are lost upon short-term inactivation of Pax5

• polyinosine-polycystosine (pIpC)-treated mice lack IgD+B220^hi cells

hematopoietic system

decreased mature B cell number ( J:75504 )

• half of the mature B cells in the spleen are absent

• only a small number of mature B cells are lost upon short-term inactivation of Pax5

• polyinosine-polycystosine (pIpC)-treated mice lack IgD+B220^hi cells

Allelic Composition	Igh^tm1(PAX5)Mbu/Igh⁺ Pax5^tm1Mbu/Pax5^tm1Mbu
Genetic Background	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh^tm1(PAX5)Mbu mutation (0 available); any Igh mutation (44 available)
Pax5^tm1Mbu mutation (1 available); any Pax5 mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell differentiation ( J:118111 )

• mice have partial block in development at pro-B cell to pre-B cells transition in bone marrow

decreased B cell number ( J:118111 )

• splenic B cell numbers were decreased by 6-fold compared to wild-type

immune system

immune system phenotype ( J:118111 )

N	• early B cell block seen in Pax5-deficient mice is rescued, as shown by presence of IgM+IgD+ mature B cells

abnormal B cell differentiation ( J:118111 )

• mice have partial block in development at pro-B cell to pre-B cells transition in bone marrow

decreased B cell number ( J:118111 )

• splenic B cell numbers were decreased by 6-fold compared to wild-type

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