Phenotypes associated with this allele
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Selltm1Tft mutation
(2 available);
any
Sell mutation
(42 available)
|
|
|
immune system
|
|
• average path length and period of leukocyte locomotion in response to superfusion of the cremaster muscle with platelet-activating factor is reduced by 50% in mutants compared to controls
• average path length of leukocyte locomotion in response to superfusion of the cremaster muscle with the murine chemokine KC is reduced by 50% in mutant mice
• period of migration of leukocytes through extravascular tissue in respone to the murine chemokine KC is shortened by 50% in mutants
• directional migration response of mutant leukocytes the a murine chemokine KC is reduced relative to wild-type
|
|
|
• in response to a 60-minute platelet-activating factor superfusion of the cremaster muscle, number of leukocytes exiting the vasculature is markedly attenuated relative to wild-type
|
cellular
|
|
• average path length and period of leukocyte locomotion in response to superfusion of the cremaster muscle with platelet-activating factor is reduced by 50% in mutants compared to controls
• average path length of leukocyte locomotion in response to superfusion of the cremaster muscle with the murine chemokine KC is reduced by 50% in mutant mice
• period of migration of leukocytes through extravascular tissue in respone to the murine chemokine KC is shortened by 50% in mutants
• directional migration response of mutant leukocytes the a murine chemokine KC is reduced relative to wild-type
|
|
|
• in response to a 60-minute platelet-activating factor superfusion of the cremaster muscle, number of leukocytes exiting the vasculature is markedly attenuated relative to wild-type
|
hematopoietic system
|
|
• average path length and period of leukocyte locomotion in response to superfusion of the cremaster muscle with platelet-activating factor is reduced by 50% in mutants compared to controls
• average path length of leukocyte locomotion in response to superfusion of the cremaster muscle with the murine chemokine KC is reduced by 50% in mutant mice
• period of migration of leukocytes through extravascular tissue in respone to the murine chemokine KC is shortened by 50% in mutants
• directional migration response of mutant leukocytes the a murine chemokine KC is reduced relative to wild-type
|
|
|
• in response to a 60-minute platelet-activating factor superfusion of the cremaster muscle, number of leukocytes exiting the vasculature is markedly attenuated relative to wild-type
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Selltm1Tft mutation
(2 available);
any
Sell mutation
(42 available)
|
|
|
hematopoietic system
|
|
• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 55% compared to wild-type
|
|
|
• frequency of rolling leukocytes is reduced by ~50% compared to wild-type at times >30 minutes after surgery to exteriorize cremaster muscle
• Tnfa (TNFalpha) treatment reduces leukocyte rolling flux fraction by 34% compared to wild-type
• rolling velocities of leukocytes are significantly lower at times <30 minutes after surgery compared to wild-type mice (19 vs 36 um/second) and remain slower at later times after surgery
|
|
|
• the cellularity of the spleen is increased by 60% compared to controls partially due to an increase in the number of T cells
• there is a 80% increase in the number of CD4 T cells found in the spleen
• there is a 30% increase in the number of T regulatory cells found in the spleen
|
|
|
• the number of regulatory T cells within peripheral lymph nodes is reduced by 90%, similar to the reduction in numbers of total CD4 T cells
• there is a 30% increase in the number of T regulatory cells found in the spleen
• the relative frequency of CD25+Foxp3+ cells was increased almost 4-fold among CD4+ T cells when compared with wild-type littermates
• 35-40% of peripheral lymph node CD4+ T cells are Treg cells
• the relative frequency of CD4 T cells within the mesenteric lymph nodes is 25% compared to 14% in wild-type mice
• the overall number of T regulatory cells in the spleen is increased by 29% though the relative frequency is reduced
|
|
|
• number of circulating monocytes is increased over wild-type (146% of wild-type number)
(J:48271)
• the number of monocytes in the blood are double that found in controls
(J:142184)
|
|
|
• migration of regulatory T cells to peripheral and mesenteric lymph nodes is greatly impaired by 75% or more
|
immune system
|
|
• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 55% compared to wild-type
|
|
|
• frequency of rolling leukocytes is reduced by ~50% compared to wild-type at times >30 minutes after surgery to exteriorize cremaster muscle
• Tnfa (TNFalpha) treatment reduces leukocyte rolling flux fraction by 34% compared to wild-type
• rolling velocities of leukocytes are significantly lower at times <30 minutes after surgery compared to wild-type mice (19 vs 36 um/second) and remain slower at later times after surgery
|
|
|
• the cellularity of the spleen is increased by 60% compared to controls partially due to an increase in the number of T cells
• there is a 80% increase in the number of CD4 T cells found in the spleen
• there is a 30% increase in the number of T regulatory cells found in the spleen
|
|
|
• the number of regulatory T cells within peripheral lymph nodes is reduced by 90%, similar to the reduction in numbers of total CD4 T cells
• there is a 30% increase in the number of T regulatory cells found in the spleen
• the relative frequency of CD25+Foxp3+ cells was increased almost 4-fold among CD4+ T cells when compared with wild-type littermates
• 35-40% of peripheral lymph node CD4+ T cells are Treg cells
• the relative frequency of CD4 T cells within the mesenteric lymph nodes is 25% compared to 14% in wild-type mice
• the overall number of T regulatory cells in the spleen is increased by 29% though the relative frequency is reduced
|
|
|
• number of circulating monocytes is increased over wild-type (146% of wild-type number)
(J:48271)
• the number of monocytes in the blood are double that found in controls
(J:142184)
|
|
|
• migration of regulatory T cells to peripheral and mesenteric lymph nodes is greatly impaired by 75% or more
|
|
|
• peripheral lymph node cellularity is reduced by 97%
(J:141931)
• the number of CD4 T cells found in peripheral lymph nodes is reduced by 96%
(J:141931)
• there is a 20-fold reduction in the cellularity of peripheral lymph nodes and a 2-fold reduction in cellularity of mesenteric lymph nodes
(J:142184)
|
neoplasm
|
|
• when injected with melanoma cells, mice exhibit a 3.9 fold increase in tumor volume on day 7 compared to wild-type mice
|
cellular
|
|
• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 55% compared to wild-type
|
|
|
• frequency of rolling leukocytes is reduced by ~50% compared to wild-type at times >30 minutes after surgery to exteriorize cremaster muscle
• Tnfa (TNFalpha) treatment reduces leukocyte rolling flux fraction by 34% compared to wild-type
• rolling velocities of leukocytes are significantly lower at times <30 minutes after surgery compared to wild-type mice (19 vs 36 um/second) and remain slower at later times after surgery
|
growth/size/body
|
|
• the cellularity of the spleen is increased by 60% compared to controls partially due to an increase in the number of T cells
• there is a 80% increase in the number of CD4 T cells found in the spleen
• there is a 30% increase in the number of T regulatory cells found in the spleen
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Selltm1Tft mutation
(2 available);
any
Sell mutation
(42 available)
|
|
|
immune system
|
|
• at 1 hour post-injection of labeled mutant or wild-type splenocytes into mice, accumulation of mutant splenocytes in Peyer's patches, peripheral lymph nodes and mesenteric lymph nodes is significantly lower than in mice receiving control splenocytes; migration to the spleen however is increased by 25%
• at 48 hours post-injection, mutant lymphocyte migration is decreased to the peripheral (99%) and mesenteric lymph nodes (51%); significant increases in mutant lymphocyte migration to the spleen (190%) and Peyer's patches (52%) are seen
|
|
|
• total numbers of neutrophils in peritoneal exudates in mutant mice at 1,2, and 4 hours after injection of thioglycollate to induce inflammation are reduced by 78%, 72% and 36% respectively compared to control values
|
|
|
• leukocyte rolling flux is reduced by 70% in mutant mice (21.7% of leukocytes passing through venules in control mice interact with the endothelium versus 6.7% in homozygotes)
• leukocyte rolling flux is significantly decreased at 40-80 minutes after exteriorization compared to control values
|
|
|
• homozygous mice have spleens that are on average 29% larger than spleens of controls
|
hematopoietic system
|
|
• at 1 hour post-injection of labeled mutant or wild-type splenocytes into mice, accumulation of mutant splenocytes in Peyer's patches, peripheral lymph nodes and mesenteric lymph nodes is significantly lower than in mice receiving control splenocytes; migration to the spleen however is increased by 25%
• at 48 hours post-injection, mutant lymphocyte migration is decreased to the peripheral (99%) and mesenteric lymph nodes (51%); significant increases in mutant lymphocyte migration to the spleen (190%) and Peyer's patches (52%) are seen
|
|
|
• total numbers of neutrophils in peritoneal exudates in mutant mice at 1,2, and 4 hours after injection of thioglycollate to induce inflammation are reduced by 78%, 72% and 36% respectively compared to control values
|
|
|
• leukocyte rolling flux is reduced by 70% in mutant mice (21.7% of leukocytes passing through venules in control mice interact with the endothelium versus 6.7% in homozygotes)
• leukocyte rolling flux is significantly decreased at 40-80 minutes after exteriorization compared to control values
|
|
|
• homozygous mice have spleens that are on average 29% larger than spleens of controls
|
cellular
|
|
• at 1 hour post-injection of labeled mutant or wild-type splenocytes into mice, accumulation of mutant splenocytes in Peyer's patches, peripheral lymph nodes and mesenteric lymph nodes is significantly lower than in mice receiving control splenocytes; migration to the spleen however is increased by 25%
• at 48 hours post-injection, mutant lymphocyte migration is decreased to the peripheral (99%) and mesenteric lymph nodes (51%); significant increases in mutant lymphocyte migration to the spleen (190%) and Peyer's patches (52%) are seen
|
|
|
• total numbers of neutrophils in peritoneal exudates in mutant mice at 1,2, and 4 hours after injection of thioglycollate to induce inflammation are reduced by 78%, 72% and 36% respectively compared to control values
|
|
|
• leukocyte rolling flux is reduced by 70% in mutant mice (21.7% of leukocytes passing through venules in control mice interact with the endothelium versus 6.7% in homozygotes)
• leukocyte rolling flux is significantly decreased at 40-80 minutes after exteriorization compared to control values
|
growth/size/body
|
|
• homozygous mice have spleens that are on average 29% larger than spleens of controls
|
growth/size/body
|
|
• mice are ~20% heavier than wild-type or Selltm1Tft mutant mice
|
immune system
|
|
• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 92% compared to wild-type
|
|
|
• tumor necrosis factor alpha (Tnfa) treatment reduces leukocyte rolling flux below level seen in Selltm1Tft mutant mice
• rolling velocities of leukocytes are significantly greater at times <30 minutes after surgery compared to Selltm1Tft mutant mice (40 vs 19 um/second)
|
|
|
• increased significantly compared to wild-type, comparable to Icam1-deficient mice
|
|
|
• number of circulating neutrophils is increased over wild-type (580% of wild-type number)
|
|
|
• number of circulating lymphocytes is increased over wild-type (200% of wild-type number)
|
|
|
• number of circulating monocytes is increased over wild-type (640% of wild-type number)
|
|
|
• mast cell infiltration of tissue after wounding is lower in mutants with or without Sele/Selp blockade at 3 and 7 days compared to wild-type animals; bFGF or PDGF signifcantly increase mast cell infiltration at 3 and 7 days in mutants with Sele/Selp blockade
|
|
|
• macrophage infiltration is reduced at 3 and 7 days after wounding in mutants with or without Sele/Selp blockade relative to wild-type; numbers are reduced to greater degree with Sele/Selp blockade in mutants at 3 days but difference is not significant at 7 days
|
|
|
• numbers in tissue after wounding are lower in mutants compared to controls
• bFGF or PDGFsignifcantly increase macrophage infiltration at 3 and 7 days in mutants with Sele/Selp blockade
|
|
|
• after wounding, levels of cytokine mRNAs including TNFalpha, Il6, Il10, and TGFbeta are all reduced in mutants with or without Sele/Selp blockade compared to controls
• bFGF or PDGF significantly increases CD3+ T cell infiltration at 3 and 7 days in mutants with Sele/Selp blockade
|
hematopoietic system
|
|
• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 92% compared to wild-type
|
|
|
• tumor necrosis factor alpha (Tnfa) treatment reduces leukocyte rolling flux below level seen in Selltm1Tft mutant mice
• rolling velocities of leukocytes are significantly greater at times <30 minutes after surgery compared to Selltm1Tft mutant mice (40 vs 19 um/second)
|
|
|
• increased significantly compared to wild-type, comparable to Icam1-deficient mice
|
|
|
• number of circulating neutrophils is increased over wild-type (580% of wild-type number)
|
|
|
• number of circulating lymphocytes is increased over wild-type (200% of wild-type number)
|
|
|
• number of circulating monocytes is increased over wild-type (640% of wild-type number)
|
|
|
• mast cell infiltration of tissue after wounding is lower in mutants with or without Sele/Selp blockade at 3 and 7 days compared to wild-type animals; bFGF or PDGF signifcantly increase mast cell infiltration at 3 and 7 days in mutants with Sele/Selp blockade
|
|
|
• macrophage infiltration is reduced at 3 and 7 days after wounding in mutants with or without Sele/Selp blockade relative to wild-type; numbers are reduced to greater degree with Sele/Selp blockade in mutants at 3 days but difference is not significant at 7 days
|
|
|
• numbers in tissue after wounding are lower in mutants compared to controls
• bFGF or PDGFsignifcantly increase macrophage infiltration at 3 and 7 days in mutants with Sele/Selp blockade
|
neoplasm
|
|
• when injected with melanoma cells, mice exhibit a 2.3 fold increase in tumor volume on day 7 compared to wild-type mice; difference in tumor volume is 5.8-fold compared to wild-type on day 14
|
cardiovascular system
|
|
• at 3 and 7 days following injury, vascular density in the wound bed in mutants with or without Sele and Selp blockade is less than that observed in wild-type controls; treatment with bFGF increases vascular vessel density at 3 days after wounding in mutants with Sele/Selp blockade, but this is not maintained at 7 days
|
homeostasis/metabolism
integument
|
|
• keratinocyte migration in wound healing is inhibited in mutants at 3 and 7 days compared to wild-type mice, and is further inhibited by Sele and Selp blockade; treatment with bFGF almost normalized keratinocyte migration in wounds by day 7 in mutants with Sele/Selp blockade
|
cellular
|
|
• numbers in tissue after wounding are lower in mutants compared to controls
• bFGF or PDGFsignifcantly increase macrophage infiltration at 3 and 7 days in mutants with Sele/Selp blockade
|
|
|
• keratinocyte migration in wound healing is inhibited in mutants at 3 and 7 days compared to wild-type mice, and is further inhibited by Sele and Selp blockade; treatment with bFGF almost normalized keratinocyte migration in wounds by day 7 in mutants with Sele/Selp blockade
|
|
|
• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 92% compared to wild-type
|
|
|
• tumor necrosis factor alpha (Tnfa) treatment reduces leukocyte rolling flux below level seen in Selltm1Tft mutant mice
• rolling velocities of leukocytes are significantly greater at times <30 minutes after surgery compared to Selltm1Tft mutant mice (40 vs 19 um/second)
|
Analysis Tools