Phenotypes associated with this allele

• Allele Symbol: Spi1^tm1Ram
• Allele Name: targeted mutation 1, Richard A Maki
• Allele ID: MGI:1927876
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Spi1^tm1Ram/Spi1^tm1Ram	involves: 129S2/SvPas	MGI:2658721
hm2	Spi1^tm1Ram/Spi1^tm1Ram	involves: 129S2/SvPas * C57BL/6	MGI:3793505
ht3	Spi1^tm1Ram/Spi1^+	involves: 129S2/SvPas	MGI:2658728
cx4	Spi1^tm1Ram/Spi1^tm1Ram Tg(SOD1*G93A)1Gur/0	involves: 129S2/SvPas * C57BL/6 * SJL	MGI:3793622

Genotype
MGI:2658721

hm1

• Allelic Composition: Spi1^tm1Ram/Spi1^tm1Ram
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

abnormal eye development ( J:101493 )

• the pupillary membrane and tunica vasculosa lentis persist postnatally unlike in wild-type mice due to a defective clearance of apoptotic cells

persistence of hyaloid vascular system ( J:101493 )

hematopoietic system

decreased macrophage cell number ( J:101493 )

• mice lack ocular macrophages

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:95801 )

N • despite a lack of macrophages, wound healing occurs at the same rate as in wild-type mice

immune system

decreased macrophage cell number ( J:101493 )

• mice lack ocular macrophages

Genotype
MGI:3793505

hm2

• Allelic Composition: Spi1^tm1Ram/Spi1^tm1Ram
• Genetic Background: involves: 129S2/SvPas * C57BL/6

mortality/aging

premature death ( J:116087 )

• antibiotic-treated mice receiving bone marrow transplants have a life expectancy of 331 +/- 20 days

neonatal lethality, complete penetrance ( J:36473 )

• all mice develop septicemia and die within 24 hours of birth, but mice treated with antibiotics live up to 17 days of age

immune system

abnormal neutrophil differentiation ( J:36473 )

• no mature neutrophils are found in the blood, liver, spleen or bone marrow and fewer immature neutrophils are identified

abnormal macrophage differentiation ( J:36473 )

• no mature macrophages are detected

abnormal T cell differentiation ( J:36473 )

• T cell development is delayed with T cells appearing 3 to 5 days after birth in antibiotic treated mice

decreased double-positive T cell number ( J:36473 )

• in the thymus and periphery

decreased neutrophil cell number ( J:36473 )

• no mature neutrophils are found in the blood, liver, spleen or bone marrow and fewer immature neutrophils are identified

abnormal B cell number ( J:36473 )

absent mature B cells ( J:36473 )

abnormal T cell number ( J:36473 )

decreased thymocyte number ( J:36473 )

• in the thymus and periphery

decreased CD4-positive, alpha-beta T cell number ( J:36473 )

• in the thymus and periphery

decreased CD8-positive, alpha-beta T cell number ( J:36473 )

• in the thymus and periphery

decreased macrophage cell number ( J:36473 )

• no mature macrophages are detected

abnormal microglial cell morphology ( J:116087 )

• microglia exhibit more prominent, rounded cell bodies and shorter or thicker processes indicating a less differentiated state

decreased spleen red pulp amount ( J:36473 )

sepsis ( J:36473 )

• all mice develop septicemia and die within 24 hours of birth

liver/biliary system

abnormal liver morphology ( J:36473 )

• occasionally mice exhibit a slightly darker red liver than wild-type mice

nervous system

abnormal microglial cell morphology ( J:116087 )

• microglia exhibit more prominent, rounded cell bodies and shorter or thicker processes indicating a less differentiated state

abnormal astrocyte morphology ( J:116087 )

• bone marrow donor cells fail to develop into astrocytes

growth/size/body

postnatal growth retardation ( J:116087 )

• antibiotic-treated mice receiving bone marrow cells from Tg(SOD1*G93A)1Gur mice growing slower than those receiving wild-type bone marrow cells

hematopoietic system

abnormal neutrophil differentiation ( J:36473 )

• no mature neutrophils are found in the blood, liver, spleen or bone marrow and fewer immature neutrophils are identified

abnormal macrophage differentiation ( J:36473 )

• no mature macrophages are detected

abnormal T cell differentiation ( J:36473 )

• T cell development is delayed with T cells appearing 3 to 5 days after birth in antibiotic treated mice

decreased neutrophil cell number ( J:36473 )

• no mature neutrophils are found in the blood, liver, spleen or bone marrow and fewer immature neutrophils are identified

abnormal B cell number ( J:36473 )

absent mature B cells ( J:36473 )

abnormal T cell number ( J:36473 )

decreased thymocyte number ( J:36473 )

• in the thymus and periphery

decreased double-positive T cell number ( J:36473 )

• in the thymus and periphery

decreased CD4-positive, alpha-beta T cell number ( J:36473 )

• in the thymus and periphery

decreased CD8-positive, alpha-beta T cell number ( J:36473 )

• in the thymus and periphery

decreased macrophage cell number ( J:36473 )

• no mature macrophages are detected

abnormal microglial cell morphology ( J:116087 )

• microglia exhibit more prominent, rounded cell bodies and shorter or thicker processes indicating a less differentiated state

decreased spleen red pulp amount ( J:36473 )

cellular

abnormal neutrophil differentiation ( J:36473 )

• no mature neutrophils are found in the blood, liver, spleen or bone marrow and fewer immature neutrophils are identified

abnormal macrophage differentiation ( J:36473 )

• no mature macrophages are detected

endocrine/exocrine glands

decreased thymocyte number ( J:36473 )

• in the thymus and periphery

Genotype
MGI:2658728

ht3

• Allelic Composition: Spi1^tm1Ram/Spi1⁺
• Genetic Background: involves: 129S2/SvPas

vision/eye

abnormal eye development ( J:101493 )

• the pupillary membrane and tunica vasculosa lentis partially persist postnatally unlike in wild-type mice due to a defective clearance of apoptotic cells

persistence of hyaloid vascular system ( J:101493 )

• partial

Genotype
MGI:3793622

cx4

• Allelic Composition: Spi1^tm1Ram/Spi1^tm1Ram; Tg(SOD1*G93A)1Gur/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * SJL

mortality/aging

premature death ( J:116087 )

• mice receiving bone marrow from when treated with wild-type bone marrow transfer survive longer than Tg(SOD1*G93A)1Gur mice or mice receiving bone marrow transfers Tg(SOD1*G93A)1Gur mice but not as long as wild-type mice

nervous system

decreased motor neuron number ( J:116087 )

• motor neuron loss is more severe in mice receiving bone marrow transfer from Tg(SOD1*G93A)1Gur mice compared to mice receiving bone marrow transfers from wild-type mice that do not exhibit a significant amount of neuron loss

motor neuron degeneration ( J:116087 )

• antibiotic-treated mice receiving bone marrow cells from wild-type donors exhibit reduced motor neuron disease progression compared to those receiving bone marrow transplants from Tg(SOD1*G93A)1Gur mice

growth/size/body

growth/size/body region phenotype ( J:116087 )

N • antibiotic-treated mice exhibit normal growth rates