Phenotypes associated with this allele

• Allele Symbol: Grin1^tm2Stl
• Allele Name: targeted mutation 2, Susumu Tonegawa
• Allele ID: MGI:1928279
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Emx1^tm1(cre)Ito/Emx1^+ Grin1^tm1Stl/Grin1^tm2Stl	involves: 129P2/OlaHsd * 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:3581525
cn2	Agrp^tm2(HBEGF)Rpa/Agrp^+ Grin1^tm2Stl/Grin1^tm2Stl	involves: 129S4/SvJae * 129S4/SvJaeSor	MGI:5427451
cn3	Grin1^tm2Stl/Grin1^tm2Stl Tg(Camk2a-cre)T29-1Stl/0	involves: 129S4/SvJae * BALB/c * C57BL/6	MGI:3581524
cn4	Grin1^tm2Stl/Grin1^tm2Stl Tg(Actb-tTA)1Jzt/0 Tg(Camk2a-cre)62Jzt/0 Tg(tetO-Grin1/GFP)1Jzt/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:3847227
cn5	Grin1^tm2Stl/Grin1^tm2Stl Tg(Pomc-cre)1Lowl/0	involves: 129S4/SvJae * C57BL/6 * FVB	MGI:4362041
cn6	Grin1^tm2Stl/Grin1^tm2Stl Tg(Ppp1r2-cre)4127Nkza/0	involves: 129S4/SvJae * C57BL/6N	MGI:4421577
cn7	Grin1^tm2Stl/Grin1^tm2Stl Tg(Grik4-cre)G32-4Stl/0	involves: C57BL/6	MGI:3582225
cn8	Grin1^tm2Stl/Grin1^tm2Stl Tg(Actb-tTA)1Jzt/0 Tg(Camk2a-cre)T29-1Stl/0 Tg(tetO-Grin1/GFP)1Jzt/0	involves: C57BL/6 * CBA	MGI:3038603

Genotype
MGI:3581525

cn1

• Allelic Composition: Emx1^tm1(cre)Ito/Emx1⁺; Grin1^tm1Stl/Grin1^tm2Stl
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal somatosensory cortex morphology ( J:64064 )

• thalamocortical afferents corresponding to large whiskers form patterns and display critical period plasticity but their pattering is not as well defined as in controls

• thalamocortical patterns corresponding to sinus hairs and digits are mostly absent

abnormal primary somatosensory cortex morphology ( J:80900 )

• dendritic fields of spiny stellate cells do not orient toward thalamocortical axon terminal patches, instead they radiate in all directions covering larger territories, exhibiting profuse branching with increased spine density

• cortex thalamocortical axons form smaller patches and individual axon terminal branching is not as well developed as in controls

• septal areas between thalamocortical axon patches are enlarged

• decreased total axonal length, number of branches (by about 50%) and lateral:vertical field span ratio of thalamocortical axons

absent barrels in primary somatosensory cortex ( J:64064 , J:80900 )

• barrels and barrel boundaries do not develop even at sites where thalamocortical afferents cluster (J:64064)

• layer IV cells of the somatosensory cortex fail to segregate into barrels (J:80900)

reduced NMDA receptor mediated synaptic activity in barrel cortex ( J:64064 )

• barrel cortex lacks NMDA receptor-mediated excitation

growth/size/body

decreased body weight ( J:64064 )

• average body weight was about 70% of that of controls at P7

abnormal postnatal growth ( J:64064 )

• low growth rate

Genotype
MGI:5427451

cn2

• Allelic Composition: Agrp^{tm2(HBEGF)Rpa}/Agrp⁺; Grin1^tm2Stl/Grin1^tm2Stl
• Genetic Background: involves: 129S4/SvJae * 129S4/SvJaeSor

behavior/neurological

abnormal eating behavior ( J:182577 )

• diphtheria-treated mice bilaterally injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus

growth/size/body

abnormal postnatal growth/weight/body size ( J:182577 )

• diphtheria-treated mice injected with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries or parabrachial nucleus prevents starvation induced by AgRP neuron ablation unlike in control mice

Genotype
MGI:3581524

cn3

• Allelic Composition: Grin1^tm2Stl/Grin1^tm2Stl; Tg(Camk2a-cre)T29-1Stl/0
• Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6

behavior/neurological

abnormal contextual conditioning behavior ( J:60730 )

• impaired hippocampus-dependent, but not hippocampus-independent, fear memory that can be rescued with enriched training

abnormal olfactory discrimination memory ( J:60730 )

• impaired olfactory-discrimination memory that can be rescued with enriched training

impaired social transmission of food preference ( J:60730 )

abnormal object recognition memory ( J:60730 )

• deficit in novel object recognition memory that can be partially rescued with enriched training

abnormal spatial learning ( J:37457 )

• impaired spatial memory in the hidden-platform Morris water maze test, with some progressive improvement in escape latencies but unimpaired nonspatial learning

nervous system

abnormal excitatory postsynaptic currents ( J:37457 )

• lack the slow component of the excitatory postsynaptic current mediated by NMDA receptors

absence of NMDA-mediated synaptic currents ( J:37457 )

• lack the slow component of the excitatory postsynaptic current mediated by NMDA receptors, however postsynaptic AMPA receptors as well as presynaptic terminals operate normally

reduced long-term potentiation ( J:37457 )

• lack long term potentiation in the CA1 synapses

Genotype
MGI:3847227

cn4

• Allelic Composition: Grin1^tm2Stl/Grin1^tm2Stl; Tg(Actb-tTA)1Jzt/0; Tg(Camk2a-cre)62Jzt/0; Tg(tetO-Grin1/GFP)1Jzt/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

behavior/neurological

abnormal learning/memory/conditioning ( J:100903 )

• consolidation and storage of long-term nondeclarative taste memories is impaired in these mice when given doxycycline prior to or within three weeks of training

abnormal conditioned taste aversion behavior ( J:100903 )

• mice given doxycycline have impairment of conditioned taste aversion

• mice are trained to avoid saccharin-containing water by being given a LiCl injection after water consumption

• control mice choose unsweetened water about 83.58% of the time 30 days after training

• mutant mice given doxycycline prior to training only choose unsweetened water 32.24% of the time

• mutant mice given doxycycline 2 weeks after training choose unsweetened water 25.63% of the time

• mutant mice given doxycycline 3 weeks to 4 weeks after training choose unsweetened water 27.99% of the time

• mutant mice given doxycycline 4 weeks after training choose unsweetened water at the same frequency as controls

Genotype
MGI:4362041

cn5

• Allelic Composition: Grin1^tm2Stl/Grin1^tm2Stl; Tg(Pomc-cre)1Lowl/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB

nervous system

nervous system phenotype ( J:122763 )

N • population responses recordings from the stimulated perforant path in the dentate hilus are not different from controls at 20-24 weeks

N • paired-pulse facilitation is normal

N • long term potentiation in area CA1 after Schaffer commissural input stimulation in CA3

N • hippocampal memory and discrimination is normal in mutants (measured by acquisition of contextual fear conditioning)

impaired synaptic plasticity ( J:122763 )

• theta-burst stimulation of the perforant path (PP) did not evoke potentiation (plasticity) of synapses of the PP-GC (dentate gyrus granule cells)

behavior/neurological

behavior/neurological phenotype ( J:122763 )

N • open field activity is not different from controls

N • no differences are seen in feeding behavior or body weights compared to controls

N • spatial learning in Morris water maze test is normal

abnormal discrimination learning ( J:122763 )

• mice show impaired acquisition of a discrimination task compared to controls; deficit is limited to early days of training, but is comparable to controls after extended training

Genotype
MGI:4421577

cn6

• Allelic Composition: Grin1^tm2Stl/Grin1^tm2Stl; Tg(Ppp1r2-cre)4127Nkza/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6N

behavior/neurological

behavior/neurological phenotype ( J:156785 )

N • adult mice (25-29 weeks of age) do not show behavioral deficits and display normal social recognition

N • mice at more than 32 weeks of age do not deficits in nest building behavior

N • mice do not show anxiety-like behaviors

nervous system

nervous system phenotype ( J:156785 )

N • mice display normal prepulse inhibition of the auditory startle reflex

N • no differences in mean firing rates or cross-correlation values of pyramidal neurons are detected relative to controls

Genotype
MGI:3582225

cn7

• Allelic Composition: Grin1^tm2Stl/Grin1^tm2Stl; Tg(Grik4-cre)G32-4Stl/0
• Genetic Background: involves: C57BL/6

behavior/neurological

abnormal spatial learning ( J:77718 )

• impaired spatial memory recall after partial cue removal but not under full cue conditions in the Morris water maze and mutants exhibited normal motivation, motor coordination and sensory functions

nervous system

abnormal single cell response ( J:77718 )

• decreased firing rate of putative CA1 interneurons

• CA1 pyramidal cells showed a significant decrease in complex spike bursting properties (complex spike index and burst spike frequency) when mutants were engaged in open-field foraging

• CA1 cells showed significant reductions in burst spike frequency, place field size and integrated firing rate after partial cue removal, however showed no significant changes when mutants were returned to an environment with all the distal cues present

abnormal NMDA-mediated synaptic currents ( J:77718 )

• the 6-cyano-7-dinitroquinoxalline-2,3-dione (DNQX)-insensitive component of the NMDA current was significantly reduced at the recurrent commissural/associational synapse relative to controls

reduced long-term potentiation ( J:77718 )

• long term potentiation was nearly absent at the commissural/associational (CA)-CA3 synapses in 9/11 mutants

Genotype
MGI:3038603

cn8

• Allelic Composition: Grin1^tm2Stl/Grin1^tm2Stl; Tg(Actb-tTA)1Jzt/0; Tg(Camk2a-cre)T29-1Stl/0; Tg(tetO-Grin1/GFP)1Jzt/0
• Genetic Background: involves: C57BL/6 * CBA

behavior/neurological

abnormal learning/memory/conditioning ( J:77659 )

• deficits in learning, memory and conditioning are seen in doxycycline treated mutants where expression of Grin1 is knocked out and expression of Grin1-GFP in the CA1 region of the hippocampus is blocked but deficits in learning, memory and conditioning are not seen in untreated mutants

abnormal contextual conditioning behavior ( J:77659 , J:88689 )

• with doxycycline treatment prior to or immediately after training mutants show significantly fewer freezing responses compared to untreated mutants in a retention test following a fear-conditioning task (J:77659)

• with doxycycline treatment just prior to testing (investigating the role of Grin1 in memory retrieval) no significant difference in a 1-month retention test following a fear-conditioning task is seen (J:77659)

• no difference in cued-fear conditioning behavior is seen with doxycycline treatment (J:77659)

• with doxycycline treatment for 30 days in the seventh month after training mutants show severe deficits in retention of remote contextual fear memory in a 9-month contextual retention test (J:88689)

• no deficit was seen with doxycycline treatment for 7 days in the seventh month after training (J:88689)

• no difference in cued-fear conditioning behavior is seen with doxycycline treatment (J:88689)

• 2 months after stopping doxycycline treatment mutants perform normally in 1-day contextual fear retention, visual memory, open field behavior and rotorod tests (J:88689)

abnormal spatial learning ( J:77659 )

• with doxycycline treatment prior to or immediately after training mutants exhibited longer escape latency in a hidden-platform water maze compared to untreated mutants

• with doxycycline treatment just prior to testing (investigating the role of Grin1 in memory retrieval) no significant difference in escape latency is seen

• deficits in spatial learning and memory are also seen in mutants with doxycycline treatment prior to testing in the transfer test compared to untreated mutants

nervous system

absence of NMDA-mediated synaptic currents ( J:77659 , J:88689 )

• with doxycycline treatment excitation postsynaptic potentials are absent in the CA1 hippocampal region (J:77659)

• no long-term potentiation is observed in doxycycline treated homozygotes (J:77659)

• with a 5 day doxycycline treatment no long-term potentiation is observed in mutants (J:88689)