Phenotypes associated with this allele

• Allele Symbol: Grin2a^tm1Rsp
• Allele Name: targeted mutation 1, Rolf Sprengel
• Allele ID: MGI:1928287
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Grin2a^tm1Rsp/Grin2a^tm1Rsp	involves: 129S1/Sv * 129X1/SvJ	MGI:3847250
cx2	Grin2a^tm1Rsp/Grin2a^tm1Rsp Grin2c^tm1Rsp/Grin2c^tm1Rsp	involves: 129S1/Sv * 129X1/SvJ	MGI:3850691

Genotype
MGI:3847250

hm1

• Allelic Composition: Grin2a^tm1Rsp/Grin2a^tm1Rsp
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired contextual conditioning behavior ( J:45446 )

• mice exhibit reduced latency in the step-down avoidance task; these mice all exhibit jumping and vocalization upon shock, suggesting that this phenotype is not attributable to differences in pain perception

decreased anxiety-related response ( J:197485 )

• in an elevated plus maze, mice spend more time in open arms compared with wild-type mice

• in an open field, mice travel more distance and take less time to enter the inner zone compared with wild-type mice

impaired coordination ( J:45446 )

• impaired motor coordination is shown by a decreased retention time on a rotarod and on a thin stationary plexiglass rod

decreased kindling response ( J:45446 )

• induction of kindling is impaired in homozygous mice; more than a 2-fold increase in stimulations is required to achieve kindling

• significant reductions in kindling-induced mossy-fiber sprouting are seen in homozygous mice

• when kindling is achieved, the duration of electrographic seizure is 2-fold greater than in controls

nervous system

nervous system phenotype ( J:45446 )

N • NMDAR-mediated synaptic currents are similar in mutant and control mice, suggesting that synaptic transmission does not account for the loss of LTP in mutant mice, and that signal transduction events from the cytoplasmic tail are involved in LTP induction

decreased kindling response ( J:45446 )

• induction of kindling is impaired in homozygous mice; more than a 2-fold increase in stimulations is required to achieve kindling

• significant reductions in kindling-induced mossy-fiber sprouting are seen in homozygous mice

• when kindling is achieved, the duration of electrographic seizure is 2-fold greater than in controls

reduced long-term potentiation ( J:45446 , J:197485 )

• in hippocampus slices derived from 3 month old mutant mice, the potentiation of synaptic response to tetanic stimulation was considerably reduced compared to controls (J:45446)

• following theta burst stimulation (J:197485)

Genotype
MGI:3850691

cx2

• Allelic Composition: Grin2a^tm1Rsp/Grin2a^tm1Rsp; Grin2c^tm1Rsp/Grin2c^tm1Rsp
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

behavior/neurological

impaired contextual conditioning behavior ( J:45446 )

• mice exhibit reduced latency in the step-down avoidance task; these mice all exhibit jumping and vocalization upon shock, suggesting that this phenotype is not attributable to differences in pain perception

impaired coordination ( J:45446 )

• impaired motor coordination is shown by a decreased retention time on a rotarod and on a thin stationary plexiglass rod

nervous system

nervous system phenotype ( J:45446 )

N • mutant mice exhibit slightly reduced cerebellar NR1 levels, but comimmunoprecipitation experiments show that the NR1 and mutant subunits are associated with each other as well as with PSD-95

N • synaptic activation of these mutant receptors evoked postsynaptic elevations of Ca2+ intracellular concentration, similar to the controls