Phenotypes associated with this allele

• Allele Symbol: Brca2^tm1Mak
• Allele Name: targeted mutation 1, Tak W Mak
• Allele ID: MGI:1930617
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Brca2^tm1Mak/Brca2^tm2Mak Trp53^tm1Brd/Trp53^tm1Brd Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * CBA	MGI:3831545
cn2	Brca2^tm1Mak/Brca2^tm2Mak Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3831544

Genotype
MGI:3831545

cn1

• Allelic Composition: Brca2^tm1Mak/Brca2^tm2Mak; Trp53^tm1Brd/Trp53^tm1Brd; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:79697 )

N • proliferating T cells fail to exhibit the same increase in spontaneous apoptosis observed in Brca2^tm1Mak/Brca2^tm2Mak Tg(Lck-cre)548Jxm mice

increased activated T cell number ( J:79697 )

• likely due to decreased apoptosis

neoplasm

increased tumor incidence ( J:79697 )

• tumorigenesis is accelerated compared to in wild-type and Trp53^tm1Brd/Trp53^tm1Brd Tg(Lck-cre)548Jxm mice but not compared to in Trp53^tm1Brd homozygotes

increased lymphoma incidence ( J:79697 )

• 90% of mice succumb to T-cell lymphomas compared to 70% of Trp53^tm1Brd/Trp53^tm1Brd Tg(Lck-cre)548Jxm mice

increased malignant tumor incidence ( J:79697 )

• mice develop high grade malignancies

increased osteosarcoma incidence ( J:79697 )

cellular

abnormal chromosome morphology ( J:79697 )

• T cells exhibit increased frequencies of chromatid or chromosomal breaks, tri-radial structures, and chromosomal fragments compared to in Brca2^tm1Mak/Brca2^tm2Mak Tg(Lck-cre)548Jxm mice or wild-type cells

decreased cellular sensitivity to ionizing radiation ( J:79697 )

• thymocytes are resistant to ionizing radiation-induced cell death unlike wild-type cells

• however, response to UV- or methyl methane sulfonate-induced cell death is normal

hematopoietic system

increased activated T cell number ( J:79697 )

• likely due to decreased apoptosis

skeleton

increased osteosarcoma incidence ( J:79697 )

Genotype
MGI:3831544

cn2

• Allelic Composition: Brca2^tm1Mak/Brca2^tm2Mak; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

immune system phenotype ( J:79697 )

N • T cell development and proliferation are normal

increased T cell apoptosis ( J:79697 )

• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells

• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal

neoplasm

neoplasm ( J:79697 )

N • mice do not develop tumors

cellular

cellular phenotype ( J:79697 )

N • response to DNA damaging agents is normal

abnormal chromosome morphology ( J:79697 )

• T cells exhibit increased frequencies of chromatid or chromosomal breaks, tri-radial structures, and chromosomal fragments compared to in wild-type cells

aneuploidy ( J:79697 )

• 14.5% of T cells exhibit aneuploidy unlike wild-type cells

increased T cell apoptosis ( J:79697 )

• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells

• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal

hematopoietic system

increased T cell apoptosis ( J:79697 )

• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells

• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal