All Phenotypes En2<tm2Alj> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

En2^tm2Alj
targeted mutation 2, Alexandra L Joyner
MGI:1930810

Summary

16 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	En2^tm2Alj/En2^tm2Alj	involves: 129S2/SvPas * C57BL/6	MGI:2175855
hm2	En2^tm2Alj/En2^tm2Alj	involves: 129S2/SvPas * Swiss Webster	MGI:3789308
ht3	En2^tm2Alj/En2^tm3Alj	involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6	MGI:2175857
cn4	En1^tm2(cre)Wrst/En1^tm8.1Alj En2^tm2Alj/En2⁺	involves: 129/Sv * Black Swiss * C57BL/6 * SJL * Swiss Webster	MGI:3789300
cn5	En1^tm2(cre)Wrst/En1^tm8.1Alj En2^tm2Alj/En2^tm2Alj	involves: 129/Sv * Black Swiss * C57BL/6 * SJL * Swiss Webster	MGI:3789301
cn6	En1^{tm7(cre/ESR1)Alj}/En1⁺ En2^tm2Alj/En2^tm2Alj Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺	involves: 129S1/Sv * 129S4/SvJaeSor * 129S6/SvEvTac * 129X1/SvJ * Swiss Webster	MGI:3789333
cx7	En1^tm2Alj/En1^tm2Alj En2^tm2Alj/En2^tm2Alj	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:2171403
cx8	En1^tm2Alj/En1^tm2Alj En2^tm2Alj/En2⁺	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789302
cx9	En1^tm2Alj/En1⁺ En2^tm2Alj/En2⁺	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789303
cx10	En1^tm2Alj/En1⁺ En2^tm2Alj/En2^tm2Alj	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789305
cx11	En1^tm5(en)Alj/En1^tm5(en)Alj En2^tm2Alj/En2^tm2Alj	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789310
cx12	En1^tm5(en)Alj/En1^tm5(en)Alj En2^tm2Alj/En2⁺	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789311
cx13	En1^tm5(en)Alj/En1⁺ En2^tm2Alj/En2^tm2Alj	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789315
cx14	En1^tm2Alj/En1^tm3(En2)Alj En2^tm2Alj/En2^tm2Alj	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789316
cx15	En1^tm3(En2)Alj/En1^tm3(En2)Alj En2^tm2Alj/En2^tm2Alj	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789317
cx16	En1^tm3(En2)Alj/En1^tm3(En2)Alj En2^tm2Alj/En2⁺	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster	MGI:3789318

Allelic Composition	En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129S2/SvPas * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal cerebellum development ( J:17203 )

• abnormal folding pattern

• fused folds in the hemispheres results in 3 rather than 4 lobules

abnormal cerebellar foliation ( J:17203 )

• altered pattern

• 3 rather than 4 major folds

• paraflocculus about half normal size

abnormal cerebellum posterior vermis morphology ( J:17203 )

• lobe VIII is associated with lobe IX rather than lobes VI and VII

small cerebellum ( J:17203 )

Allelic Composition	En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129S2/SvPas * Swiss Webster

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

decreased inferior colliculus size ( J:134516 )

• reduced in size at E18.5

• in adults, IC is truncated

abnormal cerebellum morphology ( J:134516 )

• size is slightly reduced at E11.5

abnormal cerebellum vermis morphology ( J:134516 )

• in E18.5 mutants, there is a general delay in fissure formation in vermis

• in adults, vermis is reduced in size

abnormal cerebellum posterior vermis morphology ( J:134516 )

• folium VIII position is shifted posteriorly and is associated with folium IX instead of with VI/VII

small cerebellum ( J:134516 )

• overall size reduced by one-third

Allelic Composition	En2^tm2Alj/En2^tm3Alj
Genetic Background	involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)
En2^tm3Alj mutation (0 available); any En2 mutation (104 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

nervous system phenotype ( J:60433 )

N	• brain morphology is normal

Allelic Composition	En1^tm2(cre)Wrst/En1^tm8.1Alj En2^tm2Alj/En2⁺
Genetic Background	involves: 129/Sv * Black Swiss * C57BL/6 * SJL * Swiss Webster

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1^tm2(cre)Wrst mutation (1 available); any En1 mutation (34 available)
En1^tm8.1Alj mutation (1 available); any En1 mutation (34 available)
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

absent inferior colliculus ( J:134516 )

• absent at E18.5

decreased superior colliculus size ( J:134516 )

• reduced in size at E18.5

absent cerebellum ( J:134516 )

• at E18.5, no cerebellum is present

Allelic Composition	En1^tm2(cre)Wrst/En1^tm8.1Alj En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129/Sv * Black Swiss * C57BL/6 * SJL * Swiss Webster

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

absent inferior colliculus ( J:134516 )

• absent at E18.5

absent superior colliculus ( J:134516 )

• absent at E18.5

absent colliculi ( J:134516 )

absent tectum ( J:134516 )

absent cerebellum ( J:134516 )

• at E18.5, no cerebellum is present

Allelic Composition	En1^{tm7(cre/ESR1)Alj}/En1⁺ En2^tm2Alj/En2^tm2Alj Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺
Genetic Background	involves: 129S1/Sv * 129S4/SvJaeSor * 129S6/SvEvTac * 129X1/SvJ * Swiss Webster

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1^{tm7(cre/ESR1)Alj} mutation (1 available); any En1 mutation (34 available)
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)
Gt(ROSA)26Sor^tm1Sor mutation (7 available); any Gt(ROSA)26Sor mutation (993 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

decreased rhombomere 1 size ( J:134516 )

• when tamoxifen is administered at E10.5, rhombomere 1 (r1) is reduced in size in mutants at E12.5

• anterior r1 cells contribute to more lateral regions of vermis than normal

decreased midbrain size ( J:134516 )

• when tamoxifen is administered at E10.5, mesencephalon is reduced in size at E12.5 relative to normal

• marked cells in posterior mesencephalon do not expand normally

abnormal tectum morphology ( J:134516 )

• when tamoxifen is administered at E10.5, size of marked domain is smaller than wild-type at E16.5

• in adults, marked domain in tectum is greatly reduced compared to wild-type; marked domain is restricted to remaining region of inferior colliculus

abnormal cerebellum morphology ( J:134516 )

• when tamoxifen is administered at E10.5, size of marked cell population is wider in mutants than in controls at E16.5

• in adults, marked domain is broader than normal

abnormal cerebellum vermis morphology ( J:134516 )

• in adults, vermis is reduced in size

embryo

decreased rhombomere 1 size ( J:134516 )

• when tamoxifen is administered at E10.5, rhombomere 1 (r1) is reduced in size in mutants at E12.5

• anterior r1 cells contribute to more lateral regions of vermis than normal

Allelic Composition	En1^tm2Alj/En1^tm2Alj En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1^tm2Alj mutation (1 available); any En1 mutation (34 available)
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

absent rhombomere 1 ( J:66592 )

• most or all of r1 deleted by E9.5

decreased rhombomere 1 size ( J:66592 )

• most or all of r1 deleted by E9.5

abnormal midbrain-hindbrain boundary development ( J:66592 )

• development normal through early somite stage but abnormal by 10-12 somite stage

absent midbrain-hindbrain boundary ( J:66592 )

• general deletion of the mesencephalon/anterior metencephalon region at E9.5

absent inferior colliculus ( J:134516 )

• absent at E18.5

absent superior colliculus ( J:134516 )

• absent at E18.5

absent colliculi ( J:134516 )

absent tectum ( J:134516 )

absent midbrain ( J:66592 )

• mostly, if not entirely, deleted at E9.5

small cerebellum ( J:134516 )

• reduced in overall size by one-third at E18.5

embryo

absent rhombomere 1 ( J:66592 )

• most or all of r1 deleted by E9.5

decreased rhombomere 1 size ( J:66592 )

• most or all of r1 deleted by E9.5

Allelic Composition	En1^tm2Alj/En1^tm2Alj En2^tm2Alj/En2⁺
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:134516 )

• no mutants are detected after birth

nervous system

absent inferior colliculus ( J:134516 )

• absent at E18.5

absent superior colliculus ( J:134516 )

• absent at E18.5

absent colliculi ( J:134516 )

absent tectum ( J:134516 )

absent cerebellum ( J:134516 )

• at E18.5, no cerebellum is observed

Allelic Composition	En1^tm2Alj/En1⁺ En2^tm2Alj/En2⁺
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal cerebellar foliation ( J:134516 )

• delay in cerebellum foliation is observed in adult mice

abnormal cerebellum vermis morphology ( J:134516 )

• in E18.5 mutants, there is a general delay in fissure formation in vermis; less severe than in En2^tm2Alj homozygotes

abnormal cerebellum anterior vermis morphology ( J:134516 )

• vermis displays fused folia, and folia I, II, and III are smaller than normal in adults

abnormal cerebellum posterior vermis morphology ( J:134516 )

• folium VIII is positioned between lobule VII and IX; folium VIII position is shifted posteriorly but less than in En2^tm2Alj homozygotes

small cerebellum ( J:134516 )

• reduced in size

Allelic Composition	En1^tm2Alj/En1⁺ En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:134516 )

N	• mutants survive to adulthood

nervous system

abnormal tectum morphology ( J:134516 )

• severely truncated in one mutant and fused with folia I-V remnants

absent inferior colliculus ( J:134516 )

• at E18.5, absent in 1 mutant

decreased inferior colliculus size ( J:134516 )

• at E18.5, structure is absent (1 mutant) or only small amount of tissue is present

• partially truncated in adults

small tectum ( J:134516 )

• at E11.5, truncation of tectum is greater than in En2^tm2Alj homozygotes

abnormal cerebellum morphology ( J:134516 )

• in posterior hemisphere crusil and paramedian folia are fused; in one embryo, a partial fissure separating crusil and paramedian folia is observed at E18.5

• hemispheres are reduced in size compared to En2^tm2Alj adult homozygotes

abnormal cerebellar foliation ( J:134516 )

• delay in cerebellum foliation is more severe than in En2^tm2Alj homozygotes

• there are more foliation defects adults than in En2^tm2Alj adult homozygotes or or En1^tm2Alj / En2^tm2Alj adult double heterozygotes

• in most mutants, the five anterior-most folia (I-V) are replaced by a single fold

abnormal cerebellum anterior vermis morphology ( J:134516 )

• only 1 distinct folium is present in anterior vermis

abnormal cerebellum posterior vermis morphology ( J:134516 )

• substantial deletion of folium VIII is detected in adults

• in some mutants, folium VIII is absent or reduced in size and misaligned with folium IX; in one mutant folia I-V are almost completely absent and fused to profoundly truncated tectum

absent cerebellum vermis ( J:134516 )

• in one mutant at E18.5

absent cerebellum ( J:134516 )

• at E18.5, no cerebellum is observed in some mutants

small cerebellum ( J:134516 )

• reduced in overall size by half at E18.5 in some mutants

• size reduction in adults is greater than in En2^tm2Alj or En1^tm2Alj / En2^tm2Alj adult heterozygotes

• size reduction at E11.5 is greater than in En2^tm2Alj homozygotes

Allelic Composition	En1^tm5(en)Alj/En1^tm5(en)Alj En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1^tm5(en)Alj mutation (0 available); any En1 mutation (34 available)
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:134516 )

• no mutants are detected after birth

nervous system

absent inferior colliculus ( J:134516 )

• structure does not form

absent superior colliculus ( J:134516 )

• in some mutants, structure does not form

decreased superior colliculus size ( J:134516 )

• in some mutants structure is half normal size

absent cerebellum ( J:134516 )

• at E18.5, no cerebellum is observed

Allelic Composition	En1^tm5(en)Alj/En1^tm5(en)Alj En2^tm2Alj/En2⁺
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased survivor rate ( J:134516 )

• about half of the expected number of mutants survive to adulthood

nervous system

decreased inferior colliculus size ( J:134516 )

• reduced in size in surviving adults

abnormal cerebellum anterior vermis morphology ( J:134516 )

• vermis displays fused folia, and folia I, II, and III are smaller than normal in adults

abnormal cerebellum posterior vermis morphology ( J:134516 )

• subregion VIII (posterior vermis) is not present in adults

small cerebellum ( J:134516 )

• in adults, reduced in overall size by one-third relative to controls

Allelic Composition	En1^tm5(en)Alj/En1⁺ En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

decreased inferior colliculus size ( J:134516 )

• reduced in size at E18.5

abnormal cerebellum morphology ( J:134516 )

• in posterior hemisphere crusil and paramedian folia are fused; in one embryo, a partial fissure separating crusil and paramedian folia is observed

abnormal cerebellar foliation ( J:134516 )

• foliation defects are milder than in En1^tm2Alj /+, En2^tm2Alj homozygotes; most mutants have foliation pattern similar to hemispheres of En2^tm2Alj homozygotes

abnormal cerebellum posterior vermis morphology ( J:134516 )

• subregion VIII (posterior vermis) is not present at E18.5

small cerebellum ( J:134516 )

• at E18.5, reduced in overall size by one-third relative to controls

Allelic Composition	En1^tm2Alj/En1^tm3(En2)Alj En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1^tm2Alj mutation (1 available); any En1 mutation (34 available)
En1^tm3(En2)Alj mutation (0 available); any En1 mutation (34 available)
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

decreased inferior colliculus size ( J:134516 )

• reduced in size at E18.5

abnormal cerebellum morphology ( J:134516 )

• in posterior hemisphere crusil and paramedian folia are fused; in one embryo, a partial fissure separating crusil and paramedian folia is observed

abnormal cerebellar foliation ( J:134516 )

• foliation defects are milder than in En1^tm2Alj /+, En2^tm2Alj homozygotes; most mutants have foliation pattern similar to hemispheres of En2^tm2Alj homozygotes

abnormal cerebellum posterior vermis morphology ( J:134516 )

• subregion VIII (posterior vermis) is not present at E18.5

small cerebellum ( J:134516 )

• at E18.5, reduced in overall size by one-third relative to controls

Allelic Composition	En1^tm3(En2)Alj/En1^tm3(En2)Alj En2^tm2Alj/En2^tm2Alj
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1^tm3(En2)Alj mutation (0 available); any En1 mutation (34 available)
En2^tm2Alj mutation (0 available); any En2 mutation (104 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:134516 )

N	• mice are viable into adulthood

nervous system

abnormal cerebellum morphology ( J:134516 )

• in posterior hemisphere crusil and paramedian folia are fused

abnormal cerebellar foliation ( J:134516 )

• in a small number of mutants, folium VIII is abnormal, being associated with folium IX

small cerebellum ( J:134516 )

• at E18.5, reduced in overall size by one-third relative to controls

Allelic Composition	En1^tm3(En2)Alj/En1^tm3(En2)Alj En2^tm2Alj/En2⁺
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

nervous system phenotype ( J:134516 )

N	• cerebellum and colliculi have normal morphology at E18.5

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