Phenotypes associated with this allele

• Allele Symbol: Dnmt1^tm2Enl
• Allele Name: targeted mutation 2, En Li
• Allele ID: MGI:1930971
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dnmt1^tm2Enl/Dnmt1^tm2Enl	involves: 129S4/SvJae	MGI:2657164
ht2	Dnmt1^tm1Okan/Dnmt1^tm2Enl	involves: 129S4/SvJae	MGI:3769047
ht3	Dnmt1^tm3Jae/Dnmt1^tm2Enl	involves: 129S4/SvJae * BALB/c	MGI:2657159

Genotype
MGI:2657164

hm1

• Allelic Composition: Dnmt1^tm2Enl/Dnmt1^tm2Enl
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:36234 )

nervous system

abnormal neural tube morphology ( J:36234 )

• distorted neural tube observed at E9.5

open neural tube ( J:129049 )

embryo

failure of initiation of embryo turning ( J:129049 )

embryonic growth arrest ( J:36234 , J:129049 )

• growth is arrested prior to the 8-somite stage (J:36234)

• at E9.5, mice exhibit developmental arrest with no or few somites (up to 7), no embryo turning and open neural tubes (J:129049)

abnormal neural tube morphology ( J:36234 )

• distorted neural tube observed at E9.5

open neural tube ( J:129049 )

impaired somite development ( J:36234 )

• lack visible somites at E9.5; a few advanced embryos develop 8 pairs of somites

failure of somite differentiation ( J:129049 )

cellular

decreased cell proliferation ( J:129049 )

• at E9.5, the number of proliferating cells reduced to 42% to 45%

abnormal DNA methylation ( J:36234 )

• DNA of endogenous C-type retroviruses and pericentromeric minor satellite repeats is highly demethylated with methylation level lower than in wild-type or Dnmt1^tm1Jae mice

Genotype
MGI:3769047

ht2

• Allelic Composition: Dnmt1^tm1Okan/Dnmt1^tm2Enl
• Genetic Background: involves: 129S4/SvJae

embryo

failure of initiation of embryo turning ( J:129049 )

embryonic growth arrest ( J:129049 )

• at E9.5, mice exhibit developmental arrest with no or few somites (up to 7), no embryo turning and open neural tubes

open neural tube ( J:129049 )

failure of somite differentiation ( J:129049 )

nervous system

open neural tube ( J:129049 )

cellular

decreased cell proliferation ( J:129049 )

• at E9.5, the number of proliferating cells reduced to 42% to 45%

Genotype
MGI:2657159

ht3

• Allelic Composition: Dnmt1^tm3Jae/Dnmt1^tm2Enl
• Genetic Background: involves: 129S4/SvJae * BALB/c

neoplasm

increased tumor incidence ( J:82979 )

• 80% developed aggressive thymic tumors by 4 to 8 months of age

• high frequency of chromosome 15 trisomy

growth/size/body

postnatal growth retardation ( J:82979 )

• neonates were approximately 70% the weight of Dnmt1^tm2Enl heterozygotes

reproductive system

reproductive system phenotype ( J:82979 )

N • mice were fertile