Phenotypes associated with this allele

• Allele Symbol: Fgf4^tm1Gol
• Allele Name: targeted mutation 1, Mitchell Goldfarb
• Allele ID: MGI:1931072
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fgf4^tm1Gol/Fgf4^tm1Gol	involves: C57BL/6J	MGI:5810595

Genotype
MGI:5810595

hm1

• Allelic Composition: Fgf4^tm1Gol/Fgf4^tm1Gol
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:22275 )

• embryos degenerate shortly after implantation

embryo

decreased inner cell mass proliferation ( J:22275 )

• in vitro, cultured blastocysts show severely impaired inner cell mass (ICM) proliferation

• cultured E2.5 morulae develop to the expanded blastocyst stage but display slow ICM regression

• however, ICM growth is rescued when morulae are cultured in the presence of exogenous FGF4 protein

abnormal embryo development ( J:22275 )

• embryos reach the blastocyst stage by E3.5 with apparently normal ICM and trophectoderm; however, by E5.5-E6.5, embryos are represented by implantation sites containing little or no embryonic material

embryonic growth arrest ( J:22275 )

• embryos undergo implantation and decidualization but fail to develop thereafter

abnormal egg cylinder morphology ( J:22275 )

• E5.5 egg cylinders are small and defective containing few embryonic cells

abnormal extraembryonic endoderm formation ( J:22275 )

• in vitro, blastocyst outgrowths lack an ICM and an outer layer of extraembryonic endoderm

• however, FGF4-treated morula outgrowths show substantial ICM growth and differentiation to extraembryonic endoderm

reproductive system

empty decidua capsularis ( J:22275 )

• E6.5 decidua lack discernible embryonic structures and contain degenerated implantation sites

cellular

decreased inner cell mass proliferation ( J:22275 )

• in vitro, cultured blastocysts show severely impaired inner cell mass (ICM) proliferation

• cultured E2.5 morulae develop to the expanded blastocyst stage but display slow ICM regression

• however, ICM growth is rescued when morulae are cultured in the presence of exogenous FGF4 protein