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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Il13tm1Anjm
targeted mutation 1, Andrew NJ McKenzie
MGI:1931821
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Il13tm1Anjm/Il13tm1Anjm C.129P2-Il13tm1Anjm MGI:3818435
hm2
 		Il13tm1Anjm/Il13tm1Anjm involves: 129P2/OlaHsd * C57BL/6 MGI:3689892
cx3
 		Il13tm1Anjm/Il13+
Il4tm1.1Wep/Il4+ 		involves: 129P2/OlaHsd * BALB/c * C57BL/6 MGI:3818436
cx4
 		Il13tm1Anjm/Il13tm1Anjm
Il5tm3Anjm/Il5tm3Anjm 		involves: 129P2/OlaHsd * C57BL/6 MGI:3689879


Genotype
MGI:3818435
hm1
Allelic
Composition		 Il13tm1Anjm/Il13tm1Anjm
Genetic
Background		 C.129P2-Il13tm1Anjm
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1Anjm mutation (0 available); any Il13 mutation (30 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased interleukin-13 secretion ( J:69099 )
• CD4+ T cells fail to make IL-13
decreased interleukin-4 secretion ( J:69099 )
• CD4+ T cells primed in vitro under Th2 conditions produce less IL-4 than controls with only 6.5% expressing IL-4 compared to 17.6% in controls
• when IL-12 is added to the culture, 33.4% of wild-type CD4+ T cells produce IL-4 compared to 6.9% of mutant CD4+ T cells
• there are less differences in IL-4 production after two rounds of priming
• in cell cultures that contain IL-4, wild-type CD4+ T cells produce more IL-4 than is internalized but mutant T cells internalize IL-4 without producing sufficient quantities to replace it




Genotype
MGI:3689892
hm2
Allelic
Composition		 Il13tm1Anjm/Il13tm1Anjm
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1Anjm mutation (0 available); any Il13 mutation (30 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal intestinal goblet cell morphology ( J:82881 )
• 3-fold fewer goblet cells are found compared to wild-type

hematopoietic system
decreased eosinophil cell number ( J:82881 )
• reduction in eosinophilia is observed compared to wild-type

immune system
decreased eosinophil cell number ( J:82881 )
• reduction in eosinophilia is observed compared to wild-type
abnormal interleukin secretion ( J:82881 )
• mutants show reduced production of IL-4, IL-5, and IL-9
granulomatous inflammation ( J:82881 , J:113543 )
• reduction in granuloma volume is observed compared to wild-type, after infection with Schistosoma eggs (J:82881)
• primary granuloma formation and volumes after Schistosoma infection are reduced by >50% compared to wild-type (J:113543)
• volumes of secondary granulomas after second exposure with parasite eggs are reduced by ~50% compared to wild-type (J:113543)
increased susceptibility to parasitic infection ( J:113543 )
• primary granuloma formation and volumes after Schistosoma infection are reduced by >50% compared to wild-type
• volumes of secondary granulomas after second exposure with parasite eggs are reduced by ~50% compared to wild typ

respiratory system
pulmonary fibrosis ( J:82881 )
• fibrosis is reduced compared to wild-type

cellular
abnormal intestinal goblet cell morphology ( J:82881 )
• 3-fold fewer goblet cells are found compared to wild-type




Genotype
MGI:3818436
cx3
Allelic
Composition		 Il13tm1Anjm/Il13+
Il4tm1.1Wep/Il4+
Genetic
Background		 involves: 129P2/OlaHsd * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1Anjm mutation (0 available); any Il13 mutation (30 available)
Il4tm1.1Wep mutation (0 available); any Il4 mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased interleukin-4 secretion ( J:69099 )
• F1 crosses between mice homozygote for either allele creates offspring that has only one functional Il13 allele that is in cis with Il4tm1.1Wep GFP reporter allele
• conversely, the one functional, wild-type Il4 allele is in cis with the null Il13tm1Anjm allele
• CD4+ T cells from these mice primed in vitro under Th2 conditions have only 4.5% of T cells producing IL-4 compared to 14.8% of T cells producing IL-4 that are homozygous for wild-type Il13 and heterozygote for Il4tm1.1Wep allele
• in contrast, GFP expression is similar in primed T cells from both sets of mice (5.9% and 6.8%)
• F1 crosses between 129 mice and Il4tm1.1Wep homozygotes demonstrated the diminished IL-4 secretion was not the result of 129 polymorphisms in the Il4-Il13 genomic region




Genotype
MGI:3689879
cx4
Allelic
Composition		 Il13tm1Anjm/Il13tm1Anjm
Il5tm3Anjm/Il5tm3Anjm
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1Anjm mutation (0 available); any Il13 mutation (30 available)
Il5tm3Anjm mutation (0 available); any Il5 mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
granulomatous inflammation ( J:113543 )
• primary granuloma formation and volumes after infection are reduced ~4-fold compared to wild-type
• volumes of secondary granulomas after second exposure with parasite eggs are reduced compared to wild-type
increased susceptibility to parasitic infection ( J:113543 )
• primary granuloma formation and volumes after infection are reduced ~4-fold compared to wild-type
• volumes of secondary granulomas after second exposure with parasite eggs are reduced compared to wild-type




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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory