About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Il13tm1Anjm
targeted mutation 1, Andrew NJ McKenzie
MGI:1931821
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Il13tm1Anjm/Il13tm1Anjm C.129P2-Il13tm1Anjm MGI:3818435
hm2
 		Il13tm1Anjm/Il13tm1Anjm involves: 129P2/OlaHsd * C57BL/6 MGI:3689892
cx3
 		Il13tm1Anjm/Il13+
Il4tm1.1Wep/Il4+ 		involves: 129P2/OlaHsd * BALB/c * C57BL/6 MGI:3818436
cx4
 		Il13tm1Anjm/Il13tm1Anjm
Il5tm3Anjm/Il5tm3Anjm 		involves: 129P2/OlaHsd * C57BL/6 MGI:3689879


Genotype
MGI:3818435
hm1
Allelic
Composition		 Il13tm1Anjm/Il13tm1Anjm
Genetic
Background		 C.129P2-Il13tm1Anjm
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1Anjm mutation (0 available); any Il13 mutation (29 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased interleukin-13 secretion ( J:69099 )
• CD4+ T cells fail to make IL-13
decreased interleukin-4 secretion ( J:69099 )
• CD4+ T cells primed in vitro under Th2 conditions produce less IL-4 than controls with only 6.5% expressing IL-4 compared to 17.6% in controls
• when IL-12 is added to the culture, 33.4% of wild-type CD4+ T cells produce IL-4 compared to 6.9% of mutant CD4+ T cells
• there are less differences in IL-4 production after two rounds of priming
• in cell cultures that contain IL-4, wild-type CD4+ T cells produce more IL-4 than is internalized but mutant T cells internalize IL-4 without producing sufficient quantities to replace it




Genotype
MGI:3689892
hm2
Allelic
Composition		 Il13tm1Anjm/Il13tm1Anjm
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1Anjm mutation (0 available); any Il13 mutation (29 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal intestinal goblet cell morphology ( J:82881 )
• 3-fold fewer goblet cells are found compared to wild-type

hematopoietic system
decreased eosinophil cell number ( J:82881 )
• reduction in eosinophilia is observed compared to wild-type

immune system
decreased eosinophil cell number ( J:82881 )
• reduction in eosinophilia is observed compared to wild-type
abnormal interleukin secretion ( J:82881 )
• mutants show reduced production of IL-4, IL-5, and IL-9
granulomatous inflammation ( J:82881 , J:113543 )
• reduction in granuloma volume is observed compared to wild-type, after infection with Schistosoma eggs (J:82881)
• primary granuloma formation and volumes after Schistosoma infection are reduced by >50% compared to wild-type (J:113543)
• volumes of secondary granulomas after second exposure with parasite eggs are reduced by ~50% compared to wild-type (J:113543)
increased susceptibility to parasitic infection ( J:113543 )
• primary granuloma formation and volumes after Schistosoma infection are reduced by >50% compared to wild-type
• volumes of secondary granulomas after second exposure with parasite eggs are reduced by ~50% compared to wild typ

respiratory system
pulmonary fibrosis ( J:82881 )
• fibrosis is reduced compared to wild-type

cellular
abnormal intestinal goblet cell morphology ( J:82881 )
• 3-fold fewer goblet cells are found compared to wild-type




Genotype
MGI:3818436
cx3
Allelic
Composition		 Il13tm1Anjm/Il13+
Il4tm1.1Wep/Il4+
Genetic
Background		 involves: 129P2/OlaHsd * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1Anjm mutation (0 available); any Il13 mutation (29 available)
Il4tm1.1Wep mutation (0 available); any Il4 mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased interleukin-4 secretion ( J:69099 )
• F1 crosses between mice homozygote for either allele creates offspring that has only one functional Il13 allele that is in cis with Il4tm1.1Wep GFP reporter allele
• conversely, the one functional, wild-type Il4 allele is in cis with the null Il13tm1Anjm allele
• CD4+ T cells from these mice primed in vitro under Th2 conditions have only 4.5% of T cells producing IL-4 compared to 14.8% of T cells producing IL-4 that are homozygous for wild-type Il13 and heterozygote for Il4tm1.1Wep allele
• in contrast, GFP expression is similar in primed T cells from both sets of mice (5.9% and 6.8%)
• F1 crosses between 129 mice and Il4tm1.1Wep homozygotes demonstrated the diminished IL-4 secretion was not the result of 129 polymorphisms in the Il4-Il13 genomic region




Genotype
MGI:3689879
cx4
Allelic
Composition		 Il13tm1Anjm/Il13tm1Anjm
Il5tm3Anjm/Il5tm3Anjm
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1Anjm mutation (0 available); any Il13 mutation (29 available)
Il5tm3Anjm mutation (0 available); any Il5 mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
granulomatous inflammation ( J:113543 )
• primary granuloma formation and volumes after infection are reduced ~4-fold compared to wild-type
• volumes of secondary granulomas after second exposure with parasite eggs are reduced compared to wild-type
increased susceptibility to parasitic infection ( J:113543 )
• primary granuloma formation and volumes after infection are reduced ~4-fold compared to wild-type
• volumes of secondary granulomas after second exposure with parasite eggs are reduced compared to wild-type




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
09/24/2024
MGI 6.24 		The Jackson Laboratory