Phenotypes associated with this allele

• Allele Symbol: Ccr1^tm1Cge
• Allele Name: targeted mutation 1, Craig Gerard
• Allele ID: MGI:1931849
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ccr1^tm1Cge/Ccr1^tm1Cge	C.129S4-Ccr1^tm1Cge	MGI:5298194
hm2	Ccr1^tm1Cge/Ccr1^tm1Cge	involves: 129S4/SvJae	MGI:4360701
hm3	Ccr1^tm1Cge/Ccr1^tm1Cge	involves: 129S4/SvJae * BALB/c	MGI:5298193
hm4	Ccr1^tm1Cge/Ccr1^tm1Cge	involves: 129S4/SvJae * C57BL/6	MGI:5298192

Genotype
MGI:5298194

hm1

• Allelic Composition: Ccr1^tm1Cge/Ccr1^tm1Cge
• Genetic Background: C.129S4-Ccr1^tm1Cge

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased CD4-positive, alpha-beta T cell number ( J:95242 )

• in the lungs of mice treated with severe respiratory syncytial virus (RSV) and cockroach antigen (CRA)

decreased CD8-positive, alpha-beta T cell number ( J:95242 )

• in the lungs of mice treated with severe respiratory syncytial virus (RSV) and cockroach antigen (CRA)

decreased interleukin-13 secretion ( J:95242 )

• in the lungs of mice treated with severe respiratory syncytial virus (RSV) and cockroach antigen (CRA)

decreased susceptibility to Riboviria infection ( J:95242 )

• following allergen challenge with cockroach antigen (CRA), mice exhibit attenuation of exacerbated airway hyperresponsiveness and IL13 production, CD4+ and CD8+ T cell accumulation in the lungs induced by severe respiratory syncytial virus (RSV) compared with wild-type mice

• however, IL4 production is normal

respiratory system

decreased airway responsiveness ( J:95242 )

• following allergen challenge with cockroach antigen (CRA), mice exhibit attenuation of exacerbated airway hyperresponsiveness induced by severe respiratory syncytial virus (RSV) compared with wild-type mice

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:95242 )

• in the lungs of mice treated with severe respiratory syncytial virus (RSV) and cockroach antigen (CRA)

decreased CD8-positive, alpha-beta T cell number ( J:95242 )

• in the lungs of mice treated with severe respiratory syncytial virus (RSV) and cockroach antigen (CRA)

Genotype
MGI:4360701

hm2

• Allelic Composition: Ccr1^tm1Cge/Ccr1^tm1Cge
• Genetic Background: involves: 129S4/SvJae

immune system

immune system phenotype ( J:115119 )

N • mice exhibit a normal humoral immune response to sheep IgG

impaired neutrophil recruitment ( J:43569 )

• less neutrophils invade the pancreas than controls after pancreatitis is induced by caerulein- injections

increased IgG2a level ( J:115119 )

• in NTS-injected mice

increased susceptibility to type IV hypersensitivity reaction ( J:115119 )

• in response to sheep IgG

increased interferon-gamma secretion ( J:115119 )

• in splenocytes in the presence of sheep IgG

decreased tumor necrosis factor secretion ( J:43569 )

• less TNF is secreted by the pancreas during acute pancreatitis

• less TNF is also secreted by the lungs into the bronchoalveolar space in the secondary response to pancreatitis

increased tumor necrosis factor secretion ( J:115119 )

• from isolated splenic mononuclear cells in response to LPS

glomerulonephritis ( J:115119 )

• NTS-injected mice develop more severe crescentic glomerulonephritis compared with wild-type mice

lung inflammation ( J:43569 )

• secondary damage to lungs caused by pancreatic inflammation is reduced in these mice

• there is less thickening of the alveolar membrane, less water gain, and reduced leakage

• neutrophil influx in the bronchoalveolar mucus is also decreased

respiratory system

lung inflammation ( J:43569 )

• secondary damage to lungs caused by pancreatic inflammation is reduced in these mice

• there is less thickening of the alveolar membrane, less water gain, and reduced leakage

• neutrophil influx in the bronchoalveolar mucus is also decreased

renal/urinary system

increased urine protein level ( J:115119 )

• in NTS-injected mice

abnormal renal glomerulus morphology ( J:115119 )

• nephrotoxic serum (NTS)-injected mice develop glomerular hypercellularity, glomerulonephritis, and glomerulosclerosis compared with wild-type mice

• however, other parameters of glomerular injury (sclerosis, necrosis, and microaneurysm formation) and interstitial injury are the same as in wild-type mice

glomerulonephritis ( J:115119 )

• NTS-injected mice develop more severe crescentic glomerulonephritis compared with wild-type mice

glomerulosclerosis ( J:115119 )

• in NTS-injected mice

glomerular crescent ( J:115119 )

• in NTS-injected mice

homeostasis/metabolism

increased blood urea nitrogen level ( J:115119 )

• in NTS-injected mice

increased urine protein level ( J:115119 )

• in NTS-injected mice

hematopoietic system

impaired neutrophil recruitment ( J:43569 )

• less neutrophils invade the pancreas than controls after pancreatitis is induced by caerulein- injections

increased IgG2a level ( J:115119 )

• in NTS-injected mice

Genotype
MGI:5298193

hm3

• Allelic Composition: Ccr1^tm1Cge/Ccr1^tm1Cge
• Genetic Background: involves: 129S4/SvJae * BALB/c

mortality/aging

decreased susceptibility to bacterial infection induced morbidity/mortality ( J:169909 )

• following cecal ligation and puncture to induce sepsis

immune system

abnormal macrophage physiology ( J:169909 )

• macrophage infected in vitro with E. Coli exhibit a 7.3-fold increase in phagocytosis compared with wild-type cells

increased macrophage nitric oxide production ( J:169909 )

• when stimulated with IFN-gamma alone or IFN-gamma and LPS

abnormal circulating chemokine level ( J:169909 )

• following cecal ligation and puncture to induce sepsis, mice exhibit reduced serum CCL2, CXCL2, and CXCL1 levels compared with wild-type mice

decreased circulating interferon-gamma level ( J:169909 )

• following cecal ligation and puncture to induce sepsis

abnormal chemokine secretion ( J:169909 )

• following cecal ligation and puncture to induce sepsis, mice exhibit reduced CXCL2, CXCL1, CCL2 lavage fluid levels and increased CXCL9, CCL6, and CCL17 lavage fluid levels compared with wild-type mice

• macrophage stimulated with medium exhibit increased production of CXCL2, CCL2, CCL5, CCL6, and CCL22 compared with wild-type cells

• macrophage stimulated with LPS produce increased levels of CXCL1, CXCL2, CCL2, CCL3, CCL5, CCL6, CCL22, and CXCL10 compared with wild-type mice

decreased interleukin-10 secretion ( J:169909 )

• following cecal ligation and puncture to induce sepsis

increased interleukin-12 secretion ( J:169909 )

• in macrophage stimulated with LPS

decreased tumor necrosis factor secretion ( J:169909 )

• following cecal ligation and puncture to induce sepsis

increased tumor necrosis factor secretion ( J:169909 )

• in macrophage stimulated with LPS

decreased susceptibility to bacterial infection ( J:169909 )

• following cecal ligation and puncture to induce sepsis, mice exhibit increased survival, improved bacterial clearance, reduced bacteremia, and altered cytokine (serum: decreased IFN-gamma; lavage fluid: decreased TNF, IL10) and chemokine (serum: decreased CCL2, CXCL2, CXCL1; lavage fluid: decreased CXCL2, CXCL1, CCL2, but increased CXCL9, CCL6, and CCL17) levels compared with wild-type mice

• however, leukocyte recruitment is normal

decreased susceptibility to bacterial infection induced morbidity/mortality ( J:169909 )

• following cecal ligation and puncture to induce sepsis

homeostasis/metabolism

increased macrophage nitric oxide production ( J:169909 )

• when stimulated with IFN-gamma alone or IFN-gamma and LPS

abnormal circulating chemokine level ( J:169909 )

• following cecal ligation and puncture to induce sepsis, mice exhibit reduced serum CCL2, CXCL2, and CXCL1 levels compared with wild-type mice

decreased circulating interferon-gamma level ( J:169909 )

• following cecal ligation and puncture to induce sepsis

hematopoietic system

abnormal macrophage physiology ( J:169909 )

• macrophage infected in vitro with E. Coli exhibit a 7.3-fold increase in phagocytosis compared with wild-type cells

increased macrophage nitric oxide production ( J:169909 )

• when stimulated with IFN-gamma alone or IFN-gamma and LPS

Genotype
MGI:5298192

hm4

• Allelic Composition: Ccr1^tm1Cge/Ccr1^tm1Cge
• Genetic Background: involves: 129S4/SvJae * C57BL/6

immune system

immune system phenotype ( J:63952 )

N • mice have an intact cutaneous hypersensitivity response to DNFB challenge

cecum inflammation ( J:107677 )

• mice treated with toxin A exhibit less severe enteritis (reduced fluid accumulation, neutrophil infiltration, and epithelial damage) compared with wild-type mice

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:63952 )

decreased susceptibility to bacterial infection ( J:107677 )

• mice treated with toxin A exhibit less severe enteritis (reduced fluid accumulation, neutrophil infiltration, and epithelial damage) compared with wild-type mice

• however, fluid accumulation induced by Cholera toxin is normal

increased length of allograft survival ( J:59235 )

• mice fail to reject class II mismatch cardiac allografts and rejected class-I and class II-mismatched BALB/c cardiac allografts slowly compared with wild-type mice

digestive/alimentary system

cecum inflammation ( J:107677 )

• mice treated with toxin A exhibit less severe enteritis (reduced fluid accumulation, neutrophil infiltration, and epithelial damage) compared with wild-type mice