Phenotypes associated with this allele

• Allele Symbol: Pou2af1^tm1Rgr
• Allele Name: targeted mutation 1, Robert Roeder
• Allele ID: MGI:1931863
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pou2af1^tm1Rgr/Pou2af1^tm1Rgr	either: (involves: 129P2/OlaHsd) or (involves: 129S1/Sv)	MGI:3696429

Genotype
MGI:3696429

hm1

• Allelic Composition: Pou2af1^tm1Rgr/Pou2af1^tm1Rgr
• Genetic Background: either: (involves: 129P2/OlaHsd) or (involves: 129S1/Sv)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:35785 )

immune system

decreased B cell proliferation ( J:35785 )

• proliferative response to surface IgM crosslinking is about 4-fold lower than in wild-type

increased B cell proliferation ( J:114816 )

• pre-B cells in culture are markedly hyperproliferative in response to IL-7

abnormal B cell differentiation ( J:35785 , J:114816 )

• antigen-dependent maturation of B cells is impaired (J:35785)

• however, T-cell differentiation and function appear normal (J:35785)

• defective pre-B cell development in the bone marrow (J:114816)

arrested B cell differentiation ( J:114816 )

• partial block in the pre-B1-to-pre-B2 stage of early B cell development

decreased B cell number ( J:114816 )

• reduced cellularity of the peripheral B cell compartment and diminished numbers of B cells that recirculate to the bone marrow from the periphery

abnormal pre-B cell morphology ( J:114816 )

• exhibit reduced numbers of pre-B2 (B220+CD43-CD25+) cells and an increased proportion of B200+CD43+ pre-B1 cells

• pre-B cells exhibit reduced pre-BCR-mediated differentiation

absent spleen germinal center ( J:35785 )

increased IgM level ( J:35785 )

• serum IgM is generally higher

decreased immunoglobulin level ( J:35785 )

• show a severe deficiency in the production of secondary immunoglobulin isotypes including IgG1, IgG2a, IgG2b, IgG3, IgA, and IgE in B cells, however this is not due to a failure in isotype switching process but rather to reduced levels of transcription from normally switched immunoglobulin heavy-chain loci

decreased IgA level ( J:35785 )

decreased IgE level ( J:35785 )

decreased IgG level ( J:35785 )

• deficiency in production of IgG1, IgG2a, IgG2b and IgG3

decreased IgG1 level ( J:35785 )

decreased IgG2a level ( J:35785 )

decreased IgG2b level ( J:35785 )

decreased IgG3 level ( J:35785 )

abnormal Peyer's patch germinal center morphology ( J:35785 )

• absent germinal center

absent lymph node germinal center ( J:35785 )

• germinal centers in the lymph nodes are absent

hematopoietic system

decreased B cell proliferation ( J:35785 )

• proliferative response to surface IgM crosslinking is about 4-fold lower than in wild-type

increased B cell proliferation ( J:114816 )

• pre-B cells in culture are markedly hyperproliferative in response to IL-7

abnormal B cell differentiation ( J:35785 , J:114816 )

• antigen-dependent maturation of B cells is impaired (J:35785)

• however, T-cell differentiation and function appear normal (J:35785)

• defective pre-B cell development in the bone marrow (J:114816)

arrested B cell differentiation ( J:114816 )

• partial block in the pre-B1-to-pre-B2 stage of early B cell development

decreased B cell number ( J:114816 )

• reduced cellularity of the peripheral B cell compartment and diminished numbers of B cells that recirculate to the bone marrow from the periphery

abnormal pre-B cell morphology ( J:114816 )

• exhibit reduced numbers of pre-B2 (B220+CD43-CD25+) cells and an increased proportion of B200+CD43+ pre-B1 cells

• pre-B cells exhibit reduced pre-BCR-mediated differentiation

absent spleen germinal center ( J:35785 )

increased IgM level ( J:35785 )

• serum IgM is generally higher

decreased immunoglobulin level ( J:35785 )

• show a severe deficiency in the production of secondary immunoglobulin isotypes including IgG1, IgG2a, IgG2b, IgG3, IgA, and IgE in B cells, however this is not due to a failure in isotype switching process but rather to reduced levels of transcription from normally switched immunoglobulin heavy-chain loci

decreased IgA level ( J:35785 )

decreased IgE level ( J:35785 )

decreased IgG level ( J:35785 )

• deficiency in production of IgG1, IgG2a, IgG2b and IgG3

decreased IgG1 level ( J:35785 )

decreased IgG2a level ( J:35785 )

decreased IgG2b level ( J:35785 )

decreased IgG3 level ( J:35785 )

cellular

decreased B cell proliferation ( J:35785 )

• proliferative response to surface IgM crosslinking is about 4-fold lower than in wild-type

increased B cell proliferation ( J:114816 )

• pre-B cells in culture are markedly hyperproliferative in response to IL-7