Phenotypes associated with this allele

• Allele Symbol: F9^tm1Dws
• Allele Name: targeted mutation 1, Darrel W Stafford
• Allele ID: MGI:1932297
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	F9^tm1Dws/F9^tm1Dws	involves: 129P2/OlaHsd	MGI:4847609
ot2	F9^tm1Dws/Y	involves: 129P2/OlaHsd	MGI:4847610
ot3	F9^tm1Dws/Y	involves: 129P2/OlaHsd * C57BL/6	MGI:3662691

Genotype
MGI:4847609

hm1

• Allelic Composition: F9^tm1Dws/F9^tm1Dws
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:166332 )

♀ • rebleeding in 4 of 9 mice results in immobility and cardiovascular instability requiring euthanasia after less than 2 hours of observation

homeostasis/metabolism

abnormal blood coagulation ( J:166332 )

♀ • rebleeding in 4 of 9 mice results in immobility and cardiovascular instability requiring euthanasia after less than 2 hours of observation

increased bleeding time ( J:166332 )

♀ • mice bleed for more than 10 minutes with a secondary bleeding challenge compared with 2.7 minutes for similarly treated wild-type mice

hematopoietic system

decreased hematocrit ( J:166332 )

♀ • following bleeding experiments

Genotype
MGI:4847610

ot2

• Allelic Composition: F9^tm1Dws/Y
• Genetic Background: involves: 129P2/OlaHsd

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:166332 )

♂ • rebleeding in 4 of 9 mice results in immobility and cardiovascular instability requiring euthanasia after less than 2 hours of observation

homeostasis/metabolism

abnormal blood coagulation ( J:166332 )

♂ • rebleeding in 4 of 9 mice results in immobility and cardiovascular instability requiring euthanasia after less than 2 hours of observation

increased bleeding time ( J:166332 )

♂ • mice bleed for more than 10 minutes with a secondary bleeding challenge compared with 2.7 minutes for similarly treated wild-type mice

hematopoietic system

decreased hematocrit ( J:166332 )

♂ • following bleeding experiments

Genotype
MGI:3662691

ot3

• Allelic Composition: F9^tm1Dws/Y
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

premature death ( J:44497 )

♂ • male hemizygotes survive well if no injury occurs but occasionally exhibit sudden death during growth, mostly as a result of internal hemorrhage after normal fighting with littermates

homeostasis/metabolism

abnormal blood coagulation ( J:44497 )

♂ • male hemizygotes display a severe hemophilia B phenotype with reduced plasma clotting activity although an 8% residual activity is observed

♂ • when their tails are cut, male hemizygotes bleed and die if the wound is not cauterized

cardiovascular system

cardiovascular system phenotype ( J:80537 )

♂N • at 13 weeks of age, prohemorrhagic male hemizygotes display normal hearts with no evidence of hemosiderin deposition

hemorrhage ( J:44497 )

♂ • male hemizygotes are grossly normal but display spontaneous bleeding, as shown by hemorrhagic swelling of the top of the feet or of the footpads or the presence of pale footpads

♂ • at autopsy, male hemizygotes show massive hemorrhages, mostly subcutaneous or in the dorsal surface musculature

♂ • intra-cranial hemorrhages, hemorrhages in the axilliary and inguinal areas, hemorrhages around the salivary glands, and hemorrhages in the pericardium are also observed

internal hemorrhage ( J:44497 )

♂ • male hemizygotes display fatal internal hemorrhage after normal fighting with littermates

hematopoietic system

enlarged spleen ( J:44497 )

♂ • male hemizygotes commonly exhibit splenomegaly, indicating anemia caused by blood loss

immune system

enlarged spleen ( J:44497 )

♂ • male hemizygotes commonly exhibit splenomegaly, indicating anemia caused by blood loss

growth/size/body

enlarged spleen ( J:44497 )

♂ • male hemizygotes commonly exhibit splenomegaly, indicating anemia caused by blood loss

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
hemophilia B		DOID:12259	OMIM:306900	J:44497