Phenotypes associated with this allele

• Allele Symbol: Zp2^tm1Dean
• Allele Name: targeted mutation 1, Jurrien Dean
• Allele ID: MGI:1932591
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zp2^tm1Dean/Zp2^tm1Dean	involves: 129S1/Sv * 129X1/SvJ * CF-1	MGI:2653174
cx2	Tg(ZP2)2Dean/0 Tg(ZP3)7812Dean/? Zp2^tm1Dean/Zp2^tm1Dean Zp3^tm1Dean/Zp3^tm1Dean	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * CF-1 * FVB/N	MGI:3664455
cx3	Tg(ZP2)2Dean/0 Zp2^tm1Dean/Zp2^tm1Dean	involves: 129S1/Sv * 129X1/SvJ * CF-1 * FVB/N	MGI:3664454

Genotype
MGI:2653174

hm1

• Allelic Composition: Zp2^tm1Dean/Zp2^tm1Dean
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CF-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal cumulus oophorus morphology ( J:67817 )

♀ • disorganized cumulus-oocyte-complex

decreased tertiary ovarian follicle number ( J:67817 )

♀ • reduced number of antral follicles relative to wild-type

reproductive system

thin zona pellucida ( J:67817 )

♀ • a thin zona pellucida is observed in pre-antral follicles, but not in the later stages of folliculogenesis

abnormal cumulus oophorus morphology ( J:67817 )

♀ • disorganized cumulus-oocyte-complex

decreased tertiary ovarian follicle number ( J:67817 )

♀ • reduced number of antral follicles relative to wild-type

decreased superovulation rate ( J:67817 )

♀ • after hormonal stimulation, the number of eggs ovulated was <5% that in wild-type mice

female infertility ( J:67817 )

♀

cellular

thin zona pellucida ( J:67817 )

♀ • a thin zona pellucida is observed in pre-antral follicles, but not in the later stages of folliculogenesis

Genotype
MGI:3664455

cx2

• Allelic Composition: Tg(ZP2)2Dean/0; Tg(ZP3)7812Dean/?; Zp2^tm1Dean/Zp2^tm1Dean; Zp3^tm1Dean/Zp3^tm1Dean
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * CF-1 * FVB/N

reproductive system

reproductive system phenotype ( J:93482 )

N • ovarian histology of mice expressing human ZP2 and ZP3 in place of the native mouse proteins appears normal

N • zonae pellucidae of oocytes from mice expressing human ZP2 and ZP3 in place of their mouse orthologs appear similar histologically to those of wild-type mice; human ZP2 and ZP3 proteins are immunohistologically detectable, with mouse ZP1, throughout the zona matrix

N • cumulus-free ovulated oocytes expressing human instead of mouse ZP2 and ZP3 bind mouse sperm, but fail to bind human sperm

reduced female fertility ( J:93482 )

♀ • female mice expressing human in place of mouse ZP2 and ZP3 ovulate approximately half the number of oocytes as do wild-type females when primed with pregnant mare serum and stimulated with human chorionic gonadotrophin

decreased litter size ( J:93482 )

• although human ZP2/ZP3 transgenes rescue the sterility of female mice lacking mouse ZP2 and ZP3, their average litter size is smaller than that of wild-type or ZP2 rescue mice

embryo

abnormal embryo development ( J:93482 )

• one- and two-cell embryos having human in place of mouse ZP2 and ZP3 exhibit persistent sperm binding, even after multiple washes using a wide-bore pipet

• cleavage of mouse ZP2 following fertilization is demonstrated by increased SDS-PAGE mobility of the reduced protein in the wild-type two-cell embryo versus in the ovulated oocyte; this mobility shift fails to occur in two-cell embryos in which human ZP2 and ZP3 replace their mouse orthologs

failure of zygotic cell division ( J:93482 )

• 24 hours after in vitro insemination, only 41% of ovulated oocytes in cumulus mass from female mice expressing human in place of mouse ZP2 and ZP3 had progressed to the 2-cell stage, versus 82% of wild-type and 68% of ZP2 rescue eggs

Genotype
MGI:3664454

cx3

• Allelic Composition: Tg(ZP2)2Dean/0; Zp2^tm1Dean/Zp2^tm1Dean
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CF-1 * FVB/N

reproductive system

reproductive system phenotype ( J:93482 )

N • ovarian histology of mice expressing human ZP2 in place of mouse ZP2 appears normal

N • zonae pellucidae of oocytes from mice expressing human in place of mouse ZP2 appear similar histologically to those of wild-type mice; the human ZP2 protein and mouse ZP1 and ZP3 proteins are immunohistologically detectable throughout the zona matrix

N • cumulus-free ovulated oocytes expressing human instead of mouse ZP2 bind mouse sperm, but fail to bind human sperm

reduced female fertility ( J:93482 )

♀ • female mice expressing human in place of mouse ZP2 ovulate fewer oocytes than do wild-type or ZP2/ZP3 rescue females when primed with pregnant mare serum and stimulated with human chorionic gonadotrophin

decreased litter size ( J:93482 )

• although expression of human ZP2 restores fertility to otherwise sterile female mice lacking mouse ZP2, their average litter size is smaller than that of wild-type controls

embryo

abnormal embryo development ( J:93482 )

• one- and two-celled embryos having human in place of mouse ZP2 exhibit persistent sperm binding, even after multiple washes using a wide-bore pipet

• cleavage of mouse ZP2 following fertilization is demonstrated by increased SDS-PAGE mobility of the reduced protein in the wild-type two-cell embryo versus in the ovulated oocyte; this mobility shift fails to occur in two-cell embryos in which human ZP2 replaces its mouse ortholog

failure of zygotic cell division ( J:93482 )

• 24 hours after in vitro insemination, only 68% of ovulated oocytes in cumulus mass from female mice expressing human in place of mouse ZP2 had progressed to the 2-cell stage, versus 82% of wild-type eggs