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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Lfngtm1Rjo
targeted mutation 1, Randy L Johnson
MGI:1933737
Summary 17 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Lfngtm1Rjo/Lfngtm1Rjo B6.129S7-Lfngtm1Rjo MGI:3850730
hm2
Lfngtm1Rjo/Lfngtm1Rjo either: (involves: 129S7/SvEvBrd * C57BL/6J) or (involves: 129S7/SvEvBrd * C57BL/6J * FVB/N) MGI:3777244
hm3
Lfngtm1Rjo/Lfngtm1Rjo involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J * FVB/NJ MGI:3849433
hm4
Lfngtm1Rjo/Lfngtm1Rjo involves: 129S7/SvEvBrd MGI:4420251
hm5
Lfngtm1Rjo/Lfngtm1Rjo involves: 129S7/SvEvBrd * C57BL/6 MGI:3618278
hm6
Lfngtm1Rjo/Lfngtm1Rjo involves: 129S7/SvEvBrd * C57BL/6J MGI:3849434
cx7
Lfngtm1Rjo/Lfngtm1Rjo
Mfngtm1Cjg/Mfngtm1Cjg
B6.Cg-Lfngtm1Rjo Mfngtm1Cjg MGI:4360090
cx8
Lfngtm1Rjo/Lfngtm1Rjo
Mfngtm1Seco/Mfngtm1Seco
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J * FVB/NJ MGI:3849431
cx9
Lfngtm1Rjo/Lfngtm1Rjo
Mfngtm1Seco/Mfngtm1Seco
Rfngtm1Tfv/Rfngtm1Tfv
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J * FVB/NJ MGI:3849432
cx10
Lfngtm1Rjo/Lfngtm1Rjo
Mesp2tm15.1(Lfng)Ysa/Mesp2+
involves: 129S7/SvEvBrd MGI:4454336
cx11
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Hes7-Lfng,-EGFP)#Ysa/0
involves: 129S7/SvEvBrd MGI:4454337
cx12
Lfngtm1Rjo/Lfngtm1Rjo
Ripply2tm1.1Ysa/Ripply2tm1.1Ysa
involves: 129S7/SvEvBrd * C57BL/6 * CBA * ICR MGI:3758871
cx13
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng-LFNG)1Dihz/0
involves: 129S7/SvEvBrd * C57BL/6J * CBA MGI:4420255
cx14
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng-LFNG)2Dihz/?
involves: 129S7/SvEvBrd * C57BL/6J * CBA MGI:4420256
cx15
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng*-LFNG)2Dihz/0
involves: 129S7/SvEvBrd * C57BL/6J * CBA MGI:4420254
cx16
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng*-LFNG)1Dihz/0
involves: 129S7/SvEvBrd * C57BL/6J * CBA MGI:4420253
cx17
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng*-LFNG)1Dihz/Tg(Lfng*-LFNG)1Dihz
involves: 129S7/SvEvBrd * C57BL/6J * CBA MGI:4420252


Genotype
MGI:3850730
hm1
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Genetic
Background
B6.129S7-Lfngtm1Rjo
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
muscle
• anterograde shifts of the cranial and caudal borders of the multifidus muscles in 13 of 15 mice
• in 6 of 15 mice there is a unilateral or bilateral anterograde shift of the cranial multifidus border to C2 or C1 rather than T2
• results in the addition of new muscle segments with novel insertion sites craniad to T2
• posterograde shifts of the cranial multifidus border are also seen with cranial insertion points at only T6 or T9 rather than T2

skeleton
• fusions and bifurcations resulting in highly variable intercostal distances
• widespread vertebral defects at almost all vertebral levels
• defects are more severe in the lumbar region than in the thoracic and cervical regions
• fusions are seen
• however, the spinous process at T2 is present and distinct




Genotype
MGI:3777244
hm2
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Genetic
Background
either: (involves: 129S7/SvEvBrd * C57BL/6J) or (involves: 129S7/SvEvBrd * C57BL/6J * FVB/N)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• survival of homozygotes postnatally is only rarely observed

embryo
• at E9.5, little compartmentalization of somites is observed, with rostral and caudal cells appearing mixed
• at E10.5, somites in thoracic region fail to compartmentalize, maintaining an unsegmented pattern and this continues during secondary body formation

skeleton
• in thoracic region, multiple rib fusions are observed
• thoracic vertebrae are disorganized; vertebral condensations are irregular and misaligned
• vertebral disorganization extends through lumbar region; vertebral condensations are irregular and misaligned
• vertebral disorganization extends through sacral region; vertebral condensations are irregular and misaligned

limbs/digits/tail
• tail is severely truncated

reproductive system
• males surviving to adulthood are sterile




Genotype
MGI:3849433
hm3
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Genetic
Background
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer than expected are found at weaning however survivors are viable and fertile
• fewer than expected are found at weaning
• Background Sensitivity: survival is improved compared to mice on a mixed C57BL/6J and 129 background

skeleton

limbs/digits/tail




Genotype
MGI:4420251
hm4
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Genetic
Background
involves: 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain

mortality/aging
• relative viability compared to wild-type controls is 9%

skeleton
• have either 1 or 0 tail vertebrae
• have either 1 or 0 tail vertebrae
• at E10.5 - E11.5, sclerotomes cranial to the hindlimb buds are fused and borders never become as visible as in wild-type controls
• at E11.5 the PAX1 domain in the tail is thicker indicating limited formation of the scleretome

embryo
• embryos are rostralized,compared to wild-type
• axial extension ceases around E10.5 resulting in a truncated tail
• somites become increasingly irregular towards the end of the tail
• however, segmentation in the sacrum and adjacent tail area is regular

limbs/digits/tail
• have either 1 or 0 tail vertebrae
• have either 1 or 0 tail vertebrae
• unsegmented tail stump




Genotype
MGI:3618278
hm5
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygotes die late in embryonic lethality

skeleton
• incompletely formed vertebrae

embryo
• failure of caudal and rostral spatial definition of half-somites
• when mutant fetal liver cells is mixed 1:1 with wild-type bone marrow and transferred into lethally irradiated hosts, there is a slight reduction in the number of marginal zone B cells generated compared to controls

reproductive system
• reproductive tracts of 4 and 7 week old null mice are smaller than heterozygous littermates
• sexually mature mice had normal follicles of all sizes and abnormal follicles
• ovaries of 4 and 7 week old null mice are smaller than heterozugous littermates
• after induced ovulation and fertilization, only 9.7% of eggs became 2-cell embryos compared to 48.8% of controls
• only 2% of embryos became 4 to 8 cell embryos and none reached the blastocyst stage compared to 31.4% of controls that reached blastocysts stage
• oocytes do not complete meiotic maturation
• all females found infertile due to defects in meiotic maturation

endocrine/exocrine glands
• sexually mature mice had normal follicles of all sizes and abnormal follicles
• ovaries of 4 and 7 week old null mice are smaller than heterozugous littermates

hematopoietic system
• when mutant fetal liver cells is mixed 1:1 with wild-type bone marrow and transferred into lethally irradiated hosts, there is a slight reduction in the number of marginal zone B cells generated compared to controls

cellular
• after induced ovulation and fertilization, only 9.7% of eggs became 2-cell embryos compared to 48.8% of controls
• only 2% of embryos became 4 to 8 cell embryos and none reached the blastocyst stage compared to 31.4% of controls that reached blastocysts stage
• oocytes do not complete meiotic maturation




Genotype
MGI:3849434
hm6
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice rarely survive postnatally
• Background Sensitivity: survival is reduced compared to mice on a mixed C57BL/6, 129, and FVB/NJ background




Genotype
MGI:4360090
cx7
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Mfngtm1Cjg/Mfngtm1Cjg
Genetic
Background
B6.Cg-Lfngtm1Rjo Mfngtm1Cjg
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Mfngtm1Cjg mutation (0 available); any Mfng mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• when mutant fetal liver cells is transferred into lethally irradiated hosts, there is a 50% reduction in the number of marginal zone B cells generated compared to controls

hematopoietic system
• when mutant fetal liver cells is transferred into lethally irradiated hosts, there is a 50% reduction in the number of marginal zone B cells generated compared to controls




Genotype
MGI:3849431
cx8
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Mfngtm1Seco/Mfngtm1Seco
Genetic
Background
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Mfngtm1Seco mutation (1 available); any Mfng mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer than expected are found at weaning however survivors are viable and fertile
• fewer than expected are found at weaning
• reduction in numbers found at weaning is not greater than that of Lfngtm1Rjo single homozygous littermates

skeleton
• skeleton phenotype is not significantly different from that of Lfngtm1Rjo single homozygous mice

limbs/digits/tail




Genotype
MGI:3849432
cx9
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Mfngtm1Seco/Mfngtm1Seco
Rfngtm1Tfv/Rfngtm1Tfv
Genetic
Background
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Mfngtm1Seco mutation (1 available); any Mfng mutation (35 available)
Rfngtm1Tfv mutation (0 available); any Rfng mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer than expected are found at weaning however survivors are viable and fertile
• fewer than expected are found at weaning
• reduction in numbers found at weaning is not greater than that of Lfngtm1Rjo single homozygous littermates

skeleton
• skeleton phenotype is not significantly different from that of Lfngtm1Rjo single homozygous mice

limbs/digits/tail
N
• unlike in chick embryo experiments, no limb abnormalities are seen




Genotype
MGI:4454336
cx10
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Mesp2tm15.1(Lfng)Ysa/Mesp2+
Genetic
Background
involves: 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Mesp2tm15.1(Lfng)Ysa mutation (0 available); any Mesp2 mutation (30 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• the segmental morphology in the developing embryos is similar to that in Lfngtm1Rjo single homozygotes
• abnormal vertebral morphology along the anteroposterior axis as is seen in Lfngtm1Rjo single homozygotes




Genotype
MGI:4454337
cx11
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Hes7-Lfng,-EGFP)#Ysa/0
Genetic
Background
involves: 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Tg(Hes7-Lfng,-EGFP)#Ysa mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
N
• no defects in somite segmentation are detected unlike in null mice not carrying the transgene

skeleton
N
• no defects are detected in the skeletal system unlike in null mice not carrying the transgene




Genotype
MGI:3758871
cx12
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Ripply2tm1.1Ysa/Ripply2tm1.1Ysa
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6 * CBA * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Ripply2tm1.1Ysa mutation (1 available); any Ripply2 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• embryos show a more rostralized phenotype than Ripply2-null embryos

skeleton
• pedicals of the neural arches are almost completely absent




Genotype
MGI:4420255
cx13
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng-LFNG)1Dihz/0
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Tg(Lfng-LFNG)1Dihz mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain

mortality/aging
• survival is improved compared to null mice not carrying the transgene but still less than that of wild-type controls
• relative viability compared to wild-type controls is 33%

skeleton
• many mice have extra pair of true ribs
• many mice have extra rib-bearing thoracic vertebrae
• many mice are missing a lumbar vertebra
• many mice have extra rib-bearing thoracic vertebra indicating homeotic transformation

reproductive system




Genotype
MGI:4420256
cx14
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng-LFNG)2Dihz/?
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Tg(Lfng-LFNG)2Dihz mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Adult tail phenotype of Lfngtm1Rjo/Lfngtm1Rjo and Hes7tm1.1Dihz/Hes7tm1.1Dihz mice and the effect on the Lfng tail phenotype by transgenic expression in the stripe domain

mortality/aging
• survival is improved compared to null mice not carrying the transgene but still less than that of wild-type controls
• relative viability compared to wild-type controls is 68%

skeleton
• many mice have extra pair of true ribs
• many mice have extra rib-bearing thoracic vertebrae
• many mice are missing a lumbar vertebra
• many mice have extra rib-bearing thoracic vertebra indicating homeotic transformation

reproductive system




Genotype
MGI:4420254
cx15
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng*-LFNG)2Dihz/0
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Tg(Lfng*-LFNG)2Dihz mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain

mortality/aging
• relative viability compared to wild-type controls is 9%

skeleton
• severely disorganized and truncated axial skeleton with irregular and fused vertebrae and vertebral bodies that rarely align with the midline
• slight restoration of thoracic vertebrae morphology compared to null mice not carrying the transgene
• slight restoration of cervical vertebrae morphology compared to null mice not carrying the transgene
• slight restoration of lumbar vertebrae morphology compared to null mice not carrying the transgene
• truncated axial skeleton

limbs/digits/tail
• tails are longer than in null mice not carrying the transgene but still shorter than in wild-type controls




Genotype
MGI:4420253
cx16
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng*-LFNG)1Dihz/0
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Tg(Lfng*-LFNG)1Dihz mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain

mortality/aging
• survival is improved compared to null mice not carrying the transgene but still less than that of wild-type controls
• relative viability compared to wild-type controls is 24%

skeleton
• severely disorganized and truncated axial skeleton with irregular and fused vertebrae and vertebral bodies that rarely align with the midline
• slight restoration of thoracic vertebrae morphology compared to null mice not carrying the transgene
• slight restoration of cervical vertebrae morphology compared to null mice not carrying the transgene
• slight restoration of lumbar vertebrae morphology compared to null mice not carrying the transgene
• truncated axial skeleton

limbs/digits/tail
• tails are longer than in null mice not carrying the transgene but still shorter than in wild-type controls




Genotype
MGI:4420252
cx17
Allelic
Composition
Lfngtm1Rjo/Lfngtm1Rjo
Tg(Lfng*-LFNG)1Dihz/Tg(Lfng*-LFNG)1Dihz
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation (0 available); any Lfng mutation (32 available)
Tg(Lfng*-LFNG)1Dihz mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain

mortality/aging
• survival is improved compared to null mice not carrying the transgene but still less than that of wild-type controls
• relative viability compared to wild-type controls is 20%

skeleton
• severely disorganized and truncated axial skeleton with irregular and fused vertebrae and vertebral bodies that rarely align with the midline
• rescue of abnormalities is confined to segmentation of the tail area
• truncated axial skeleton

limbs/digits/tail





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory