Analysis Tools|
Allele Symbol Allele Name Allele ID |
Ntrk2tm2Kln targeted mutation 2, Rudiger Klein MGI:1933974 |
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| Summary |
8 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• from 2-14 months of age, mutants and controls show similar motor coordination and motor function (no differences in hind-paw clasping or rotarod performance)
• no differences in mutants and controls are detected with the inverted screen test
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• young adult (3 month-old) mutants show significant increases in distance traveled in open field tests when acutely challenged with cocaine, compared to wild-type mice
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• mutants display subtle gait differences compared to controls at 5 months, including a smaller print width for the left hind paw and differences in footfalls of 2 different paws and slight altered timely coordination between ipsilateral (right fore- and hind-) paws and girdle (right and left) hindpaws
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• by 9 months of age and after, mutants show significantly higher locomotion in open field tests compared to wild-type
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• observed when acutely challenged with cocaine at 3 months of age
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| N |
• no loss of medium-sized spiny neurons (MSNs) in the striatum is detected compared to wild-type littermates; striatal volume in normal at 12 months of age
• corticostriatal synaptic properties and striatopallidal projections are intact in mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• fewer neurons arising in hippocampus from radial glial cells after treatment with tamoxifen survive to become mature neurons
• axon elongation is unaffected
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• dendritic branching and lengths are reduced in neurons arising in the hippocampus from radial glial cells after treatment with tamoxifen
• reduced dendritic complexity 90 um from soma
• spine density is significantly reduced
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• lasts only 30-40 minutes as compared to over 90 minutes for controls
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• studied between 28 and 42 days after tamoxifen treatment
• enter the center in an open field test less frequently and spend less time there
• more time spent on the periphery in an open field test
• in an elevated + maze, fewer entries into the open arms and less time spent in the open arms
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• studied between 28 and 42 days after tamoxifen treatment
• less spontaneous activity in an open field test
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• fewer neurons arising in hippocampus from radial glial cells after treatment with tamoxifen survive to become mature neurons
• axon elongation is unaffected
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mutants show greater weight gain in the first week on a high fat diet compared to controls on the same diet
• treatment with mifepristone stabilizes mutant body weight
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• mutants gain more weight than controls on the same diet, despite no increase in food intake
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• males develop obesity around 4 months of age, while females exhibit this around 5 months
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• gonadal white adipose tissue fat pads are significantly increased in males and females
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• from 3 months of age, thickness is increased becoming highly significant at 5 months, compared to controls
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• beginning at 3 months, hypertrophy is observed, becoming significant by 5 months
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| N |
• interscapular brown adipose tissue are found in mutants and controls
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• fat mass is significantly increased in 3 month-old and 9 month-old male mutants; however, fat-free mass is unaltered
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• mutants have increased fat accumulation in the head/neck region
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• gonadal white adipose tissue fat pads are significantly increased in males and females
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• mesenteric adipose tissue fat pads are significantly increased in males and females
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| N |
• no alterations in circadian rhythm or cumulative food intake at 2 or 8 months of age
• on a high fat diet, mutants exhibit decreased food intake similarly to controls
• open field activity and elevated plus maze behavior (indicator of anxiety-like behavior) does not differ between mutants and controls at any age
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• home cage activity is significantly increased during the dark phase in 8 month-old obese mutants
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• GABAergic output of Cck interneurons onto Crh neurons is reduced; increased neuronal activity results
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| N |
• oxygen consumption and carbon dioxide production rates, as well as respiratory exchange ratio and energy expenditure are similar
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• signicantly elevated in older obese mice
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• signicantly elevated in older obese mice
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• observed in older obese mice
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• mutants show increased de novo lipogenesis in the liver
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• afternoon levels are increased compared to controls
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• gonadal white adipose tissue fat pads are significantly increased in males and females
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• aged mice (5 months) show increased hepatic lipid stores compared to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice die between 3 and 8 months
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• expression of parvalbumin in the entorhinal cortex and the hippocampus is reduced compared to in wild-type mice
• in the most superficial layers of the neocortex dendrites are fasiculated and poorly stained while in the deepest layers (V-VI) staining is reduced compared to in wild-type mice
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• in the most superficial layers of the neocortex dendrites are fasiculated and poorly stained while in the deepest layers (V-VI) staining is reduced compared to in wild-type mice
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• delayed at E18.5
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• mice exhibit severe caudal retraction of the cortical hemisphere compared to in wild-type mice
• however, the olfactory bulb and cerebellum are normal
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• at E18.5, proliferating cells are more diffusely disturbed in the central part of the cortical plate compared to in wild-type mice
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• the thickness of all layers is reduced compared to in wild-type mice
• layer II/III is slightly compressed with tightly packed neurons compared to in wild-type mice
• however, lamination is normal
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• severely compressed
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• severely compressed
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• reduced
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• in the central nervous system
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• after P21
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• expression of parvalbumin in the entorhinal cortex and the hippocampus is reduced compared to in wild-type mice
• in the most superficial layers of the neocortex dendrites are fasiculated and poorly stained while in the deepest layers (V-VI) staining is reduced compared to in wild-type mice
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• in the most superficial layers of the neocortex dendrites are fasiculated and poorly stained while in the deepest layers (V-VI) staining is reduced compared to in wild-type mice
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• delayed at E18.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• a certain degree of task-specific long-term memory appears to be present but mutants show increasingly impaired learning behavior or inappropriate coping response when facing complex and/or stressful learning paradigms such as in contextual fear conditioning, two-way avoidance, radial maze and water maze learning
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• in avoidance learning, mutants first react normally to the new environment but fall into a persisting maladaptive locomotor activity
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• in the contextual fear conditioning, mutants fail to develop an appropriate freezing response after 30 min but show correct freezing after 24 hours yet not following exposure to tone
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• mutants show impaired spatial learning in the Morris water maze test and partial impairment in the eight-arm radial maze
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• mutants exhibit persistent thigmotaxis in the water maze
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• the number of myelinated axons in the CA1 region of the hippocampus is reduced by 23%
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• reduction in LTP at CA1 hippocampal synapses
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• basal synaptic transmission is normal but synaptic strengthening is impaired as evidenced by abnormal long term potentiation
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• there is a reduced induction rate of LTP in hippocampal slices
• induction (measured as a signal 20% above baseline) only occurs 37.0% of the time after burst stimulation compared to 70% in controls
• similar results are observed after tetanus application
• impairment also occurs after long-lasting LTP (L-LTP) that is tested by repeated theta burst
• L-LTP induction rate is 14.2% compared to 62.5% and does not improve with increased stimulus strength
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• synaptic contacts appear to be normal for neurons arising in hippocampus from radial glial cells after treatment with tamoxifen
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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