Phenotypes associated with this allele

• Allele Symbol: Il15ra^tm1Ama
• Allele Name: targeted mutation 1, Averil Ma
• Allele ID: MGI:1934016
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Il15ra^tm1Ama/Il15ra^tm1Ama	involves: 129X1/SvJ	MGI:3702076
cn2	Il15ra^tm1Ama/Il15ra^tm2.1Ama Lyz2^tm1(cre)Ifo/0 Tg(Itgax-cre)1-1Reiz/0	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * CBA	MGI:4417844
cn3	Il15ra^tm1Ama/Il15ra^tm2.1Ama Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6J	MGI:4417843
cn4	Il15ra^tm1Ama/Il15ra^tm2.1Ama Tg(Itgax-cre)1-1Reiz/0	involves: 129X1/SvJ * C57BL/6J * CBA	MGI:4417842
cn5	Il15ra^tm1Ama/Il15ra^tm2.1Ama Tg(Vil1-cre)997Gum/0	involves: C57BL/6J * SJL	MGI:4417845

Genotype
MGI:3702076

hm1

• Allelic Composition: Il15ra^tm1Ama/Il15ra^tm1Ama
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:142570 )

• TCR-activated CD8+ T cells stimulated with 5 ng/mL IL15 exhibit little if any proliferation while stimulation with 50 ng/mL induces reduced proliferation compared to similarly treated wild-type cells

• however, stimulation of TCR-activated CD8+ T cells with 500 ng/mL IL15 induces normal proliferation

• donor CD4+ and CD8+ T cells transplanted into irradiated wild-type mice exhibit reduced cell-cycle progression compared to donor wild-type cellsdonor CD4+ and CD8+ T cells transplanted into irradiated wild-type mice exhibit reduced cell-cycle progression compared to donor wild-type cells

decreased NK cell number ( J:119213 , J:142570 )

• in 6 to 12 week-old mice, percentages of NK cells in spleen and bone marrow (BM) or mutants are extremely low (J:119213)

• however, when spleen and BM progenitors are cultured with appropriate factors (Il-7, SCF, flt3) followed by Il-15, they can differentiate into functional NK cells, if transplanted into wild-type recipients, spleen and BM cells can differentiate in vivo to NK cells (J:119213)

• mice exhibit a reduction in splenic NK cells compared to in wild-type mice (J:142570)

decreased CD8-positive, alpha-beta T cell number ( J:142570 )

• mice exhibit a reduction in CD8+ T cells compared to in wild-type mice

decreased NK T cell number ( J:142570 )

• mice exhibit a reduction in NKT and thymic NKT cells compared to in wild-type mice

decreased CD8-positive, gamma-delta intraepithelial T cell number ( J:155298 )

• marked reduction

decreased memory T cell number ( J:142570 )

• mice exhibit a reduction in CD44^highIL23beta+CD8+ memory T cells compared to in wild-type mice

abnormal NK cell physiology ( J:119213 )

• NK cells generated in vitro do not express Ly-49 receptors as normal NK cells do; NK cells differentiated in vivo from transplanted mutant splenic and BM cells show much lower expression of Ly-49 than wild-type NK cells

impaired natural killer cell mediated cytotoxicity ( J:142570 )

• mice have no cytolytic NK activity

hematopoietic system

decreased T cell proliferation ( J:142570 )

• TCR-activated CD8+ T cells stimulated with 5 ng/mL IL15 exhibit little if any proliferation while stimulation with 50 ng/mL induces reduced proliferation compared to similarly treated wild-type cells

• however, stimulation of TCR-activated CD8+ T cells with 500 ng/mL IL15 induces normal proliferation

• donor CD4+ and CD8+ T cells transplanted into irradiated wild-type mice exhibit reduced cell-cycle progression compared to donor wild-type cellsdonor CD4+ and CD8+ T cells transplanted into irradiated wild-type mice exhibit reduced cell-cycle progression compared to donor wild-type cells

decreased NK cell number ( J:119213 , J:142570 )

• in 6 to 12 week-old mice, percentages of NK cells in spleen and bone marrow (BM) or mutants are extremely low (J:119213)

• however, when spleen and BM progenitors are cultured with appropriate factors (Il-7, SCF, flt3) followed by Il-15, they can differentiate into functional NK cells, if transplanted into wild-type recipients, spleen and BM cells can differentiate in vivo to NK cells (J:119213)

• mice exhibit a reduction in splenic NK cells compared to in wild-type mice (J:142570)

decreased CD8-positive, alpha-beta T cell number ( J:142570 )

• mice exhibit a reduction in CD8+ T cells compared to in wild-type mice

decreased NK T cell number ( J:142570 )

• mice exhibit a reduction in NKT and thymic NKT cells compared to in wild-type mice

decreased CD8-positive, gamma-delta intraepithelial T cell number ( J:155298 )

• marked reduction

decreased memory T cell number ( J:142570 )

• mice exhibit a reduction in CD44^highIL23beta+CD8+ memory T cells compared to in wild-type mice

abnormal NK cell physiology ( J:119213 )

• NK cells generated in vitro do not express Ly-49 receptors as normal NK cells do; NK cells differentiated in vivo from transplanted mutant splenic and BM cells show much lower expression of Ly-49 than wild-type NK cells

impaired natural killer cell mediated cytotoxicity ( J:142570 )

• mice have no cytolytic NK activity

cellular

decreased T cell proliferation ( J:142570 )

• TCR-activated CD8+ T cells stimulated with 5 ng/mL IL15 exhibit little if any proliferation while stimulation with 50 ng/mL induces reduced proliferation compared to similarly treated wild-type cells

• however, stimulation of TCR-activated CD8+ T cells with 500 ng/mL IL15 induces normal proliferation

• donor CD4+ and CD8+ T cells transplanted into irradiated wild-type mice exhibit reduced cell-cycle progression compared to donor wild-type cellsdonor CD4+ and CD8+ T cells transplanted into irradiated wild-type mice exhibit reduced cell-cycle progression compared to donor wild-type cells

Genotype
MGI:4417844

cn2

• Allelic Composition: Il15ra^tm1Ama/Il15ra^tm2.1Ama; Lyz2^tm1(cre)Ifo/0; Tg(Itgax-cre)1-1Reiz/0
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * CBA

immune system

decreased NK cell number ( J:155298 )

• about half the number of NK cells in the spleen, lymph node, liver, and lung

• but no decrease is seen in the bone marrow

decreased CD8-positive, alpha-beta T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

decreased memory T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

abnormal immune system physiology ( J:155298 )

• decrease in the numbers of adoptively transferred transgenic CD8+ T cells compared to controls by 30 days after immunization

hematopoietic system

decreased NK cell number ( J:155298 )

• about half the number of NK cells in the spleen, lymph node, liver, and lung

• but no decrease is seen in the bone marrow

decreased CD8-positive, alpha-beta T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

decreased memory T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

Genotype
MGI:4417843

cn3

• Allelic Composition: Il15ra^tm1Ama/Il15ra^tm2.1Ama; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6J

hematopoietic system

abnormal NK cell differentiation ( J:155298 )

• increase in immature CD11b+ CXCR3+ NK cells in spleen and bone marrow

• more mature KLRG-1+ CD11b+ NK cells are underrepresented in spleen and bone marrow

decreased NK cell number ( J:155298 )

• about half the number of NK cells in the spleen, lymph node, liver, and lung

• but no decrease is seen in the bone marrow

decreased CD8-positive, alpha-beta T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

decreased memory T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

abnormal NK cell physiology ( J:155298 )

• decrease in proliferation of CD27+ CD11b+ cells

• decrease in activation of adoptively transferred wild-type NK cells in response to lipopolysaccaride

immune system

abnormal NK cell differentiation ( J:155298 )

• increase in immature CD11b+ CXCR3+ NK cells in spleen and bone marrow

• more mature KLRG-1+ CD11b+ NK cells are underrepresented in spleen and bone marrow

decreased NK cell number ( J:155298 )

• about half the number of NK cells in the spleen, lymph node, liver, and lung

• but no decrease is seen in the bone marrow

decreased CD8-positive, alpha-beta T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

decreased memory T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

abnormal immune system physiology ( J:155298 )

• exaggerated contraction of adoptively transferred transgenic CD8+ T cells seen as early as 10 days after immunization

abnormal NK cell physiology ( J:155298 )

• decrease in proliferation of CD27+ CD11b+ cells

• decrease in activation of adoptively transferred wild-type NK cells in response to lipopolysaccaride

Genotype
MGI:4417842

cn4

• Allelic Composition: Il15ra^tm1Ama/Il15ra^tm2.1Ama; Tg(Itgax-cre)1-1Reiz/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6J * CBA

hematopoietic system

abnormal NK cell differentiation ( J:155298 )

• increase in immature CD11b+ CXCR3+ NK cells in spleen and bone marrow

• more mature KLRG-1+ CD11b+ NK cells are underrepresented in spleen and bone marrow

decreased NK cell number ( J:155298 )

• about half the number of NK cells in the spleen, lymph node, liver, and lung

• but no decrease is seen in the bone marrow

abnormal T cell subpopulation ratio ( J:155298 )

• marked decrease in the ratio of CD62L^hi to CD62L^lo memory phenotype CD8+ T cells

decreased CD8-positive, alpha-beta T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

• marked decrease in the number of CD62L^hi memory phenotype CD8+ T cells

decreased memory T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

• marked decrease in the number of CD62L^hi memory phenotype CD8+ T cells

abnormal NK cell physiology ( J:155298 )

• decrease in proliferation of CD27+ CD11b+ cells

• decrease in activation of adoptively transferred wild-type NK cells in response to lipopolysaccaride

immune system

abnormal NK cell differentiation ( J:155298 )

• increase in immature CD11b+ CXCR3+ NK cells in spleen and bone marrow

• more mature KLRG-1+ CD11b+ NK cells are underrepresented in spleen and bone marrow

decreased NK cell number ( J:155298 )

• about half the number of NK cells in the spleen, lymph node, liver, and lung

• but no decrease is seen in the bone marrow

abnormal T cell subpopulation ratio ( J:155298 )

• marked decrease in the ratio of CD62L^hi to CD62L^lo memory phenotype CD8+ T cells

decreased CD8-positive, alpha-beta T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

• marked decrease in the number of CD62L^hi memory phenotype CD8+ T cells

decreased memory T cell number ( J:155298 )

• decrease in numbers of memory phenotype CD8+ T cells, particularly in lymph nodes, spleen, and blood

• marked decrease in the number of CD62L^hi memory phenotype CD8+ T cells

abnormal NK cell physiology ( J:155298 )

• decrease in proliferation of CD27+ CD11b+ cells

• decrease in activation of adoptively transferred wild-type NK cells in response to lipopolysaccaride

Genotype
MGI:4417845

cn5

• Allelic Composition: Il15ra^tm1Ama/Il15ra^tm2.1Ama; Tg(Vil1-cre)997Gum/0
• Genetic Background: involves: C57BL/6J * SJL

hematopoietic system

decreased CD8-positive, gamma-delta intraepithelial T cell number ( J:155298 )

• marked reduction

immune system

decreased CD8-positive, gamma-delta intraepithelial T cell number ( J:155298 )

• marked reduction