Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
P2rx3tm1Ckn mutation
(1 available);
any
P2rx3 mutation
(21 available)
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respiratory system
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N |
• adult homozygotes (4-6 months of age) display normal resting ventilation during normoxia as well as normal ventilatory responses to varying levels of hypoxia (15%, 10%, and 7.5% O2) relative to wild-type controls
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cardiovascular system
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N |
• adult homozygotes display normal carotid body function, as shown by a similar hypoxia-induced increase in sinus nerve activity in carotid body-sinus nerve preparations from homozygous mutant and wild-type mice
• no significant differences in the single-unit response to hypoxia are observed between mutant and wild-type carotid body-sinus nerve preparations (7.73 +/- 0.69 spikes/sec versus 8.22 +/- 0.74 spikes/sec, respectively)
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mortality/aging
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• only ~10% of double homozygotes survive through weaning, without displaying any gross pathological abnormalities in adulthood
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• >90% of double homozygotes die between birth and weaning showing pulmonary infection and bronchial pneumonia
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respiratory system
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N |
• surviving double homozygotes display normal ventilatory responses to varying levels of hypercapnia relative to wild-type controls: no significant differences in respiratory rate (fR) and tidal volume (VT) are noted at hypercapnic conditions when CO2 levels increase to 3% and then to 6%
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• double homozygotes that die between birth and weaning exhibit bronchial pneumonia, as shown by postmortem examination
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• in response to hypoxia (10% O2), adult double homozygotes (4-6 months of age) display a significantly reduced ventilatory response showing an increase of only 129 +/- 154 ml/min/kg in minute ventilation (VE) relative to 979 +/- 161 ml/min/kg in wild-type controls
• in response to further hypoxia (7.5% O2), double homozygotes display a severe ventilatory depression with the VE reduced below prehypoxic levels by 555 +/- 137 ml/min/kg, unlike wild-type controls where no additional increase in ventilation is observed but the VE remains above normoxic levels
• however, adult double homozygotes display normal resting ventilation during normoxia and show normal ventilatory responses to a mild (15% O2) hypoxia relative to wild-type controls
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cardiovascular system
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• unlike in in vitro carotid body-sinus nerve preparations from wild-type mice where sinus nerve discharge increases from baseline to a peak of 130.82 +/- 10.79 spikes/sec during hypoxia, the afferent discharge in preparations from double mutant mice only reaches a peak of 32.80 +/- 6.97 spikes/sec; this response is even smaller than that observed in preparations from single P2rx2tm1Ckn mice (58.13 +/- 9.40 spikes/sec)
• the average peak firing rate of single units induced by hypoxia is significantly lower in carotid body-sinus nerve preparations from double mutant mice (1.19 +/- 0.10 spikes/sec) relative to that observed in preparations from wild-type (7.76 +/- 0.99 spikes/sec) and single P2rx2tm1Ckn mutant mice (1.64 +/- 0.11 spikes/sec)
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immune system
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• double homozygotes that die between birth and weaning exhibit bronchial pneumonia, as shown by postmortem examination
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homeostasis/metabolism
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