Phenotypes associated with this allele

• Allele Symbol: Cxcr4^tm1Qma
• Allele Name: targeted mutation 1, Qing Ma
• Allele ID: MGI:1934166
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cxcr4^tm1Qma/Cxcr4^tm1Qma	B6.129X-Cxcr4^tm1Qma/J	MGI:3617772
hm2	Cxcr4^tm1Qma/Cxcr4^tm1Qma	involves: 129X1/SvJ	MGI:2175786
hm3	Cxcr4^tm1Qma/Cxcr4^tm1Qma	involves: 129X1/SvJ * C57BL/6	MGI:5317847
cx4	Cxcr4^tm1Qma/Cxcr4^tm1Qma Gab1^tm1Wbm/Gab1^tm1Wbm	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6	MGI:3617774
cx5	Ccr5^tm1Kuz/Ccr5^tm1Kuz Cxcr4^tm1Qma/Cxcr4^tm1Qma	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6	MGI:5317843

Genotype
MGI:3617772

hm1

• Allelic Composition: Cxcr4^tm1Qma/Cxcr4^tm1Qma
• Genetic Background: B6.129X-Cxcr4^tm1Qma/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

muscle

abnormal muscle precursor cell migration ( J:100909 )

• at E10.75 fewer hypaxial muscle progenitor cells reach the floor of the first branchial arch

• at E10.75, fewer progenitor cells are seen in the dorsal limb and this reduction is more pronounced distally (35%) than proximally (25%)

• however, no significant change in proliferation of muscle progenitors is seen

abnormal myogenesis ( J:100909 )

• at E10.75, the number of muscle cells (MyoD+) is reduced in the dorsal limb and this reduction is more pronounced distally than proximally; however at E13.5 the size and distribution of the muscle groups is not significantly different from control

• increased apoptosis is seen in the proximal domain of the dorsal limb

• however, no significant difference is seen in the size of the tongue muscle

cellular

abnormal muscle precursor cell migration ( J:100909 )

• at E10.75 fewer hypaxial muscle progenitor cells reach the floor of the first branchial arch

• at E10.75, fewer progenitor cells are seen in the dorsal limb and this reduction is more pronounced distally (35%) than proximally (25%)

• however, no significant change in proliferation of muscle progenitors is seen

Genotype
MGI:2175786

hm2

• Allelic Composition: Cxcr4^tm1Qma/Cxcr4^tm1Qma
• Genetic Background: involves: 129X1/SvJ

mortality/aging

perinatal lethality, incomplete penetrance ( J:49070 )

• about 1/3 of mice are dead at E18.5

nervous system

abnormal cerebellum morphology ( J:49070 )

• at E18.5, the cerebellum is disorganized with a highly distorted architecture and chromophillic cell clumps in the analage

• chromophillic clusters mostly contain early granule cell progenitors that are surrounded by Purkinje cells

absent cerebellar foliation ( J:49070 )

thin external granule cell layer ( J:49070 )

• at E18.5, the external granule cell layer is attenuated and irregular

ectopic Purkinje cell ( J:49070 )

hematopoietic system

decreased pro-B cell number ( J:49070 )

• at E18.5, the B220+/CD43+ pro-B cell population is severely reduced in the fetal liver (0.2% versus 7.0% in wild-type littermates); however T cell populations in the thymus are normal

impaired myelopoiesis ( J:49070 )

• at E18.5 in the fetal liver, myeloid cells are reduced to 25% of wild-type, myelopoiesis is reduced and a marked erythroid predominance is seen

• at E18.5, a marked reduction in myeloid cells is also seen in the spleen and myeloid cells are virtually absent from the bone marrow

decreased bone marrow cell number ( J:49070 )

• at E15.5, bone marrow is hypocellular and composed mostly of stromal cells and osteoblasts with a severe reduction in hematopoiesis

• at E18.5, hematopoiesis is still reduced but cellularity is similar to wild-type; however myeloid cells are virtually absent

growth/size/body

decreased body size ( J:49070 )

• viable homozygotes are smaller than littermates

renal/urinary system

kidney vascular congestion ( J:49070 )

kidney hemorrhage ( J:49070 )

• prominent interstitial hemorrhage

respiratory system

atelectasis ( J:49070 )

• collapsed lungs

immune system

decreased pro-B cell number ( J:49070 )

• at E18.5, the B220+/CD43+ pro-B cell population is severely reduced in the fetal liver (0.2% versus 7.0% in wild-type littermates); however T cell populations in the thymus are normal

impaired myelopoiesis ( J:49070 )

• at E18.5 in the fetal liver, myeloid cells are reduced to 25% of wild-type, myelopoiesis is reduced and a marked erythroid predominance is seen

• at E18.5, a marked reduction in myeloid cells is also seen in the spleen and myeloid cells are virtually absent from the bone marrow

cardiovascular system

kidney vascular congestion ( J:49070 )

kidney hemorrhage ( J:49070 )

• prominent interstitial hemorrhage

Genotype
MGI:5317847

hm3

• Allelic Composition: Cxcr4^tm1Qma/Cxcr4^tm1Qma
• Genetic Background: involves: 129X1/SvJ * C57BL/6

nervous system

abnormal nervous system physiology ( J:132342 )

• abrogated neurotoxicity to gp120 from a CCR4 preferring isolate of HIV

• decreased survival from gp120 from a CCR5 preferring isolate of HIV

immune system

increased susceptibility to Retroviridae infection induced morbidity/mortality ( J:132342 )

• decreased survival from gp120 from a CCR5 preferring isolate of HIV

decreased susceptibility to Retroviridae infection ( J:132342 )

• abrogated neurotoxicity to gp120 from a CCR5 preferring isolate of HIV

mortality/aging

increased susceptibility to Retroviridae infection induced morbidity/mortality ( J:132342 )

• decreased survival from gp120 from a CCR5 preferring isolate of HIV

Genotype
MGI:3617774

cx4

• Allelic Composition: Cxcr4^tm1Qma/Cxcr4^tm1Qma; Gab1^tm1Wbm/Gab1^tm1Wbm
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6

muscle

abnormal muscle precursor cell migration ( J:100909 )

• at E10.75 and E11.5, no hypaxial muscle progenitor cells reach the floor of the first branchial arch

abnormal intrinsic tongue muscle morphology ( J:100909 )

• only a fragment of the intrinsic muscle is present in the proximal tongue

abnormal myogenesis ( J:100909 )

• at E10.75, the number of muscle cells (MyoD+) is further reduced and the number of apoptotic cells is increased in the dorsal and ventral limbs compared to Gab single homozygotes

hypaxial muscle hypoplasia ( J:100909 )

• limb muscle size is reduced and in the proximal portion of the lower forelimb, particular extensor muscles of dorsal limb are absent and the flexor muscles are reduced in size compared to Gab1 single homozygotes

digestive/alimentary system

abnormal intrinsic tongue muscle morphology ( J:100909 )

• only a fragment of the intrinsic muscle is present in the proximal tongue

decreased tongue size ( J:100909 )

• the overall size of the tongue is very small

craniofacial

abnormal intrinsic tongue muscle morphology ( J:100909 )

• only a fragment of the intrinsic muscle is present in the proximal tongue

decreased tongue size ( J:100909 )

• the overall size of the tongue is very small

cellular

abnormal muscle precursor cell migration ( J:100909 )

• at E10.75 and E11.5, no hypaxial muscle progenitor cells reach the floor of the first branchial arch

growth/size/body

abnormal intrinsic tongue muscle morphology ( J:100909 )

• only a fragment of the intrinsic muscle is present in the proximal tongue

decreased tongue size ( J:100909 )

• the overall size of the tongue is very small

Genotype
MGI:5317843

cx5

• Allelic Composition: Ccr5^tm1Kuz/Ccr5^tm1Kuz; Cxcr4^tm1Qma/Cxcr4^tm1Qma
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6

nervous system

abnormal nervous system physiology ( J:132342 )

• abrogated neurotoxicity to gp120 from a CCR5 preferring isolate of HIV

• abrogated neurotoxicity to gp120 from a CCR4 preferring isolate of HIV

immune system

decreased susceptibility to Retroviridae infection ( J:132342 )

• abrogated neurotoxicity to gp120 from a CCR5 preferring isolate of HIV

• abrogated neurotoxicity to gp120 from a CCR4 preferring isolate of HIV