Phenotypes associated with this allele

• Allele Symbol: Cripto^tm1Mms
• Allele Name: targeted mutation 1, Michael M Shen
• Allele ID: MGI:1934206
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cripto^tm1Mms/Cripto^tm1Mms	either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss)	MGI:3612874
ht2	Cripto^tm1Mms/Cripto^tm2Mms	involves: 129S6/SvEvTac * C57BL/6	MGI:3613772

Genotype
MGI:3612874

hm1

• Allelic Composition: Cripto^tm1Mms/Cripto^tm1Mms
• Genetic Background: either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:50343 )

• homozygotes are found at the expected Mendelian frequency at E6.75 through E8.5; however, no homozygotes are obtained after E10.5

embryo

abnormal gastrulation ( J:50343 )

abnormal germ layer development ( J:50343 )

• at E7.5-E8.5, mutant embryos exhibit impaired formation of embryonic mesoderm and endoderm; instead, they largely consist of anterior neuroectoderm resulting in a "head-without-trunk" phenotype

abnormal mesoderm development ( J:50343 )

abnormal rostral-caudal axis patterning ( J:50343 )

• homozygotes fail to exhibit rotation of the anterior-posterior axis, resulting in mislocalization of the head- and trunk-organizing centers

absent mesoderm ( J:50343 )

• at E6.75, mutant embryos show absence of embryonic mesoderm; in contrast, extra-embryonic mesoderm is often present, with an allantois and blood islands of the visceral yolk sac mesoderm detected at later stages (E7.5-E8.5)

abnormal embryonic neuroepithelium morphology ( J:50343 )

• at E7.5-E8.5, homozygotes exhibit distally located ectopic folds of neuroectodermal tissue

failure of primitive streak formation ( J:50343 )

• at E6.75, homozygotes show absence of a primitive streak

absent somites ( J:50343 )

• at E7.5-E8.5, homozygotes display absence of somite formation

abnormal visceral endoderm morphology ( J:50343 )

• at E6.75, homozygotes display a thickened layer of visceral endoderm at the distal tip of the egg cylinder

cardiovascular system

abnormal heart development ( J:50343 )

• at E7.5-E8.5, homozygotes display absence of embryonic mesoderm-derived cardiac tissue

nervous system

abnormal embryonic neuroepithelium morphology ( J:50343 )

• at E7.5-E8.5, homozygotes exhibit distally located ectopic folds of neuroectodermal tissue

Genotype
MGI:3613772

ht2

• Allelic Composition: Cripto^tm1Mms/Cripto^tm2Mms
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

mortality/aging

premature death ( J:104418 )

• fewer than expected mutants are found at 4 weeks of age (8% rather than 25%)

embryo

abnormal endoderm development ( J:104418 )

• at E7.5 expression of Hex, a marker of anterior definitive endoderm, is often undetectable; however Hex expression in visceral endoderm is normal

• at E8.25 expression of Shh marking ventral midline structures and gut endoderm is greatly reduced or absent

• these changes in expression indicate defects in axial mesendoderm and definitive endoderm development with a nearly complete loss of definitive endoderm in severely affected embryos

abnormal mesendoderm development ( J:104418 )

• defects in axial mesendoderm development

rostral body truncation ( J:104418 )

• severely affected embryos show anterior truncations

• less affected embryos display anterior restricted phenotypes

absent anterior definitive endoderm ( J:104418 )

• at E7.5 expression of Hex, a marker of anterior definitive endoderm, is often undetectable

absent notochord ( J:104418 )

• in severely affected embryos the anterior somites are fused across the midline indicating the absence of the notochord

absent prechordal plate ( J:104418 )

• at E7.75 an intact prechordal plate fails to form

fused somites ( J:104418 )

• in severely affected embryos the anterior somites are fused across the midline indicating the absence of the notochord

nervous system

holoprosencephaly ( J:104418 )

• at E8.25 - E9.5, about 56% display a broad spectrum of phenotypes with characteristic features of holoprosencephaly

• at E11.5 severely affected embryos have a single prosencephalic vesicle

decreased forebrain size ( J:104418 )

• at E9.0 - E11.5 less severely affected embryos have variable forebrain reductions including truncation of the rostral forebrain

decreased midbrain size ( J:104418 )

• at E9.0 - E11.5 less severely affected embryos have variable midbrain reductions

vision/eye

cyclopia ( J:104418 )

• seen in less severely affected embryos