Phenotypes associated with this allele

• Allele Symbol: C5ar1^tm1Cge
• Allele Name: targeted mutation 1, Craig Gerard
• Allele ID: MGI:1934246
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	C5ar1^tm1Cge/C5ar1^tm1Cge	B6.129S4(Cg)-C5ar1^tm1Cge	MGI:6382542
hm2	C5ar1^tm1Cge/C5ar1^tm1Cge	involves: 129S4/SvJae	MGI:2662371
hm3	C5ar1^tm1Cge/C5ar1^tm1Cge	involves: 129S4/SvJae * C57BL/6	MGI:5295998
cx4	C3ar1^tm1Cge/C3ar1^tm1Cge C5ar1^tm1Cge/C5ar1^tm1Cge	B6.Cg-C3ar1^tm1Cge C5ar1^tm1Cge	MGI:6382540

Genotype
MGI:6382542

hm1

• Allelic Composition: C5ar1^tm1Cge/C5ar1^tm1Cge
• Genetic Background: B6.129S4(Cg)-C5ar1^tm1Cge

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

abnormal c-wave shape ( J:214214 )

• the amplitude of the c-wave is reduced at 12 months of age, but not at 8 weeks of age

• however, no other component of the dc-ERG is different and mice show normal ERG a-waves and b-waves even at 12 months of age

homeostasis/metabolism

decreased circulating free fatty acids level ( J:214214 )

• plasma free polyunsaturdated fatty acid (linoleic acid and arachidonic acid) levels are decreased at 14 months of age

Genotype
MGI:2662371

hm2

• Allelic Composition: C5ar1^tm1Cge/C5ar1^tm1Cge
• Genetic Background: involves: 129S4/SvJae

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:69177 )

• mutants challenged with Psuedomonas aeruginosa succumb in the second day after challenge while no wild-type mice die

immune system

impaired neutrophil recruitment ( J:69177 )

• when peritoneal inflammation is induced with glycogen in mutants, a decreased number of neutrophils is elicited

abnormal humoral immune response ( J:35529 )

• after injection of chicken egg albumin in the tail and administration of antibodies to egg albumin intratracheally, the null mice exhibit almost complete protection from inflammatory response upon examination of lung tissue

increased susceptibility to bacterial infection ( J:69177 )

• mutants are unable to clear intrapulmonary-instilled Pseudomonas aeruginosa, despite an increase in neutrophil influx, and succumb to pneumonia

• however, mutants are able to clear the bacteria when challenged intraperitoneally

• mutants challenged with a sublethal dose of Pseudomonas become superinfected with secondary bacterial strains

hematopoietic system

impaired neutrophil recruitment ( J:69177 )

• when peritoneal inflammation is induced with glycogen in mutants, a decreased number of neutrophils is elicited

Genotype
MGI:5295998

hm3

• Allelic Composition: C5ar1^tm1Cge/C5ar1^tm1Cge
• Genetic Background: involves: 129S4/SvJae * C57BL/6

skeleton

decreased osteoclast cell number ( J:166640 )

• bone marrow cells incubated with 1,25(OH)2 vitamin D3 produce fewer osteoclasts than do cells from controls

immune system

decreased osteoclast cell number ( J:166640 )

• bone marrow cells incubated with 1,25(OH)2 vitamin D3 produce fewer osteoclasts than do cells from controls

decreased circulating interleukin-6 level ( J:166640 )

• Il-6 production after vitamin D3 stimulation is significantly reduced

hematopoietic system

decreased osteoclast cell number ( J:166640 )

• bone marrow cells incubated with 1,25(OH)2 vitamin D3 produce fewer osteoclasts than do cells from controls

homeostasis/metabolism

decreased circulating interleukin-6 level ( J:166640 )

• Il-6 production after vitamin D3 stimulation is significantly reduced

Genotype
MGI:6382540

cx4

• Allelic Composition: C3ar1^tm1Cge/C3ar1^tm1Cge; C5ar1^tm1Cge/C5ar1^tm1Cge
• Genetic Background: B6.Cg-C3ar1^tm1Cge C5ar1^tm1Cge

vision/eye

increased retina apoptosis ( J:214214 )

• apoptosis levels are increased in the retinas at 3 months and further increased at 14 months

• mice immunized with 4-HNE-adducted protein (HNE-MSA) show elevated cell apoptosis as indicated by elevated annexin V levels compared to wild-type mice

abnormal retina rod bipolar cell morphology ( J:214214 )

• density of rod bipolar cells is decreased at 3 months and declines further at 14 months of age

abnormal retina horizontal cell morphology ( J:214214 )

• density of horizontal cells is decreased

abnormal photoreceptor outer segment disc membrane morphology ( J:214214 )

• the normal structure of the outer segment discs is lost at 6 months of age

abnormal retina pigment epithelium morphology ( J:214214 )

• retinal pigment epithelium (RPE) cell nuclei are shrunken or apoptotic at 6 months

thin retina inner nuclear layer ( J:214214 )

• the inner nuclear layer is thinner at 3 and 14 months of age

thin retina outer nuclear layer ( J:214214 )

• outer nuclear layer thinning is seen at older ages

disorganized retina outer nuclear layer ( J:214214 )

• the outer nuclear layer is disorganized at 3 months of age

increased susceptibility to age-related retinal degeneration ( J:214214 )

• mice show progressive retinal cell loss

abnormal electroretinogram waveform feature ( J:214214 )

• the fast oscillation is reduced at 6 months of age

• mice immunized with 4-HNE-adducted protein (HNE-MSA) show a more severe reduction in ERG amplitude, however this phenotype is not related to immune responses as IL17-producting cells are diminished

decreased a-wave amplitude ( J:214214 )

• by 14 weeks of age, but not at 6 weeks, ERG a-waves are reduced and the reduction progresses with increasing age

decreased b-wave amplitude ( J:214214 )

• by 14 weeks of age, but not at 6 weeks, ERG b-waves are reduced and the reduction progresses with increasing age

abnormal c-wave shape ( J:214214 )

• the amplitude of the c-wave is reduced at 6 months of age

homeostasis/metabolism

decreased circulating free fatty acids level ( J:214214 )

• plasma free polyunsaturdated fatty acid (linoleic acid and arachidonic acid) levels are decreased at 14 months of age

decreased circulating phospholipid level ( J:214214 )

• plasma lysophosphatidylcholine levels are decreased at 3 and 14 months of age

cellular

increased retina apoptosis ( J:214214 )

• apoptosis levels are increased in the retinas at 3 months and further increased at 14 months

• mice immunized with 4-HNE-adducted protein (HNE-MSA) show elevated cell apoptosis as indicated by elevated annexin V levels compared to wild-type mice

nervous system

abnormal retina rod bipolar cell morphology ( J:214214 )

• density of rod bipolar cells is decreased at 3 months and declines further at 14 months of age

abnormal retina horizontal cell morphology ( J:214214 )

• density of horizontal cells is decreased

abnormal photoreceptor outer segment disc membrane morphology ( J:214214 )

• the normal structure of the outer segment discs is lost at 6 months of age

pigmentation

abnormal retina pigment epithelium morphology ( J:214214 )

• retinal pigment epithelium (RPE) cell nuclei are shrunken or apoptotic at 6 months