Phenotypes associated with this allele

• Allele Symbol: Flt1^tm1Msh
• Allele Name: targeted mutation 1, Masabumi Shibuya
• Allele ID: MGI:1934260
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Flt1^tm1Msh/Flt1^tm1Msh	involves: 129S4/SvJae * C57BL/6J	MGI:2177813
hm2	Flt1^tm1Msh/Flt1^tm1Msh	involves: 129S4/SvJaeSor * C57BL/6 * FVB	MGI:5013922

Genotype
MGI:2177813

hm1

• Allelic Composition: Flt1^tm1Msh/Flt1^tm1Msh
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:49507 )

N • no abnormal cardiovascular phenotype is seen

cornea vascularization ( J:114973 )

• anti-Flt1 antibody treatment of mutant corneas induces vascularization; subconjunctival injection of anti-Flt1 antibodies also elicits corneal vascularization, but not in wild-type corneas

• corneal injection of a plasmid encoding short hairpin RNA targeting the C terminus of the secreted form of Flt1 (pshRNA-sflt1) induces corneal vascularization within 3 days of injection in wild-type and mutant mice

reproductive system

reproductive system phenotype ( J:49507 )

N • no abnormal reproductive phenotype is seen

vision/eye

cornea vascularization ( J:114973 )

• anti-Flt1 antibody treatment of mutant corneas induces vascularization; subconjunctival injection of anti-Flt1 antibodies also elicits corneal vascularization, but not in wild-type corneas

• corneal injection of a plasmid encoding short hairpin RNA targeting the C terminus of the secreted form of Flt1 (pshRNA-sflt1) induces corneal vascularization within 3 days of injection in wild-type and mutant mice

immune system

impaired macrophage chemotaxis ( J:49507 )

• VEGF-induced macrophage migration is almost completely abrogated

cellular

impaired macrophage chemotaxis ( J:49507 )

• VEGF-induced macrophage migration is almost completely abrogated

hematopoietic system

impaired macrophage chemotaxis ( J:49507 )

• VEGF-induced macrophage migration is almost completely abrogated

Genotype
MGI:5013922

hm2

• Allelic Composition: Flt1^tm1Msh/Flt1^tm1Msh
• Genetic Background: involves: 129S4/SvJaeSor * C57BL/6 * FVB

behavior/neurological

abnormal sensory capabilities/reflexes/nociception ( J:172783 )

• challenge with high doses of paclitaxel by the subplantar route results in a more severe sensory deficit compared to wild-type

nervous system

neuron degeneration ( J:172783 )

• mice develop a distal neuropathy