Phenotypes associated with this allele

• Allele Symbol: Cxcl12^tm1Tng
• Allele Name: targeted mutation 1, Takashi Nagasawa
• Allele ID: MGI:1934384
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cxcl12^tm1Tng/Cxcl12^tm1Tng	B6.Cg-Cxcl12^tm1Tng	MGI:3040186
hm2	Cxcl12^tm1Tng/Cxcl12^tm1Tng	involves: 129P2/OlaHsd	MGI:3040183
cn3	Cxcl12^tm1Tng/Cxcl12^tm1.1Link Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129P2/OlaHsd * C57BL/6 * CD-1 * FVB/N	MGI:5468941
cn4	Cxcl12^tm1Tng/Cxcl12^tm1.1Link Tg(Tek-cre)1Ywa/0	involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL	MGI:5468943
cn5	Cxcl12^tm1Tng/Cxcl12^tm1.1Link Tg(Prrx1-cre)1Cjt/0	involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL/J	MGI:5468942
cn6	Cxcl12^tm1Tng/Cxcl12^tm1.1Link Tg(BGLAP-cre)1Clem/0	involves: 129P2/OlaHsd * C57BL/6J * C57BL/6NTac * FVB/N	MGI:5468940

Genotype
MGI:3040186

hm1

• Allelic Composition: Cxcl12^tm1Tng/Cxcl12^tm1Tng
• Genetic Background: B6.Cg-Cxcl12^tm1Tng

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal primordial germ cell migration ( J:83291 )

• migration of primordial germ cells in the hindgut and mesentery is normal however the number of cells in the genital ridges at E10.5 is reduced in homozygotes

• proliferation of germ cells that reach the genital ridges is normal

cellular

abnormal primordial germ cell migration ( J:83291 )

• migration of primordial germ cells in the hindgut and mesentery is normal however the number of cells in the genital ridges at E10.5 is reduced in homozygotes

• proliferation of germ cells that reach the genital ridges is normal

Genotype
MGI:3040183

hm2

• Allelic Composition: Cxcl12^tm1Tng/Cxcl12^tm1Tng
• Genetic Background: involves: 129P2/OlaHsd

integument

abnormal skin vasculature morphology ( J:195048 )

• mice exhibit a failure of nerve-vessel alignment in developing limb skin compared with wild-type mice

• however, vascular density is normal

mortality/aging

neonatal lethality, complete penetrance ( J:34750 )

• homozygotes die within 1 hour of birth

lethality throughout fetal growth and development, incomplete penetrance ( J:34750 )

• about half of the homozygous embryos die between E15.5 and E18.5

cardiovascular system

abnormal skin vasculature morphology ( J:195048 )

• mice exhibit a failure of nerve-vessel alignment in developing limb skin compared with wild-type mice

• however, vascular density is normal

abnormal angiogenesis ( J:69256 )

• the large vessels of the stomach arising from the mesenchymal vessels are absent

• the small vascular network that surrounds the stomach and the vasculature of other organs are normal

poor arterial differentiation ( J:195048 )

abnormal developmental vascular remodeling ( J:195048 )

• disorganized branching patterns in developing limb skin

abnormal vascular smooth muscle morphology ( J:195048 )

• reduced smooth muscle cell coverage of small diameter branched vessels

• however, coverage of large diameter veins is normal

perimembraneous ventricular septal defect ( J:34750 )

• homozygotes have a defect in the membranous portion of the ventricular septum

hematopoietic system

decreased pro-B cell number ( J:34750 )

• pro-B cell numbers are severely reduced in the fetal livers of mutants compared to wild-type mice

impaired myelopoiesis ( J:49070 )

• at E18.5 in the fetal liver, myelopoiesis is reduced identical to the phenotype in Cxcr4^tm1Qma homozygotes

• at E18.5, a marked reduction in myeloid cells is also seen in the spleen and myeloid cells are virtually absent from the bone marrow

decreased bone marrow cell number ( J:34750 , J:49070 )

• on E17.5 hematopoietic cells are virtually absent from the bone marrow (J:34750)

• B-cell lymphopoiesis is also absent in the bone marrow (J:34750)

• at E18.5, myeloid cells are virtually absent from the bone marrow (J:49070)

decreased pre-B cell number ( J:34750 )

• by E16.5 pre-B cell numbers are severely reduced in the fetal livers of mutants compared to wild-type mice

nervous system

nervous system phenotype ( J:195048 )

N • mice exhibit normal innervation accompanied by migrating Schwann cells

abnormal cerebellum morphology ( J:49070 )

• at E18.5, the cerebellum is disorganized

thin external granule cell layer ( J:49070 )

• at E18.5, the external granule cell layer is attenuated

immune system

decreased pro-B cell number ( J:34750 )

• pro-B cell numbers are severely reduced in the fetal livers of mutants compared to wild-type mice

impaired myelopoiesis ( J:49070 )

• at E18.5 in the fetal liver, myelopoiesis is reduced identical to the phenotype in Cxcr4^tm1Qma homozygotes

• at E18.5, a marked reduction in myeloid cells is also seen in the spleen and myeloid cells are virtually absent from the bone marrow

decreased pre-B cell number ( J:34750 )

• by E16.5 pre-B cell numbers are severely reduced in the fetal livers of mutants compared to wild-type mice

muscle

abnormal vascular smooth muscle morphology ( J:195048 )

• reduced smooth muscle cell coverage of small diameter branched vessels

• however, coverage of large diameter veins is normal

Genotype
MGI:5468941

cn3

• Allelic Composition: Cxcl12^tm1Tng/Cxcl12^tm1.1Link; Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CD-1 * FVB/N

hematopoietic system

abnormal blood cell morphology/development ( J:193594 )

• the numbers of colony-forming cells and KSL cells are increased in the blood and spleen, demonstrating constitutive HPC mobilization, compared with control mice

decreased pre-pro B cell number ( J:193594 )

decreased bone marrow cell number ( J:193594 )

decreased B cell number ( J:193594 )

• in the bone marrow

decreased hematopoietic stem cell number ( J:193594 )

• modest decrease in the absolute number

abnormal hematopoietic stem cell physiology ( J:193594 )

• increased cycling of more mature KSL progenitors in the blood

• however, hematopoietic stem cell cycling is normal

immune system

decreased pre-pro B cell number ( J:193594 )

decreased B cell number ( J:193594 )

• in the bone marrow

Genotype
MGI:5468943

cn4

• Allelic Composition: Cxcl12^tm1Tng/Cxcl12^tm1.1Link; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL

hematopoietic system

hematopoietic system phenotype ( J:193594 )

N • hematopoietic stem cell numbers and cycling are normal

abnormal bone marrow cell physiology ( J:193594 )

• bone marrow cells exhibit multilineage long-term repopulating defects

• however, self-renewal capacity is restored by transplantation into a wild-type environment

Genotype
MGI:5468942

cn5

• Allelic Composition: Cxcl12^tm1Tng/Cxcl12^tm1.1Link; Tg(Prrx1-cre)1Cjt/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL/J

hematopoietic system

decreased common myeloid progenitor cell number ( J:193594 )

• 2-fold

decreased pre-pro B cell number ( J:193594 )

decreased bone marrow cell number ( J:193594 )

abnormal common lymphocyte progenitor cell morphology ( J:193594 )

• reduced B cell lymphoid progenitors

decreased B cell number ( J:193594 )

• in the bone marrow

decreased hematopoietic stem cell number ( J:193594 )

abnormal bone marrow cell physiology ( J:193594 )

• bone marrow cells exhibit multilineage long-term repopulating defects

• however, self-renewal capacity is restored by transplantation into a wild-type environment

abnormal hematopoietic stem cell physiology ( J:193594 )

• increased cycling of hematopoietic stem cells and more mature KSL progenitors in the spleen

immune system

decreased pre-pro B cell number ( J:193594 )

decreased B cell number ( J:193594 )

• in the bone marrow

Genotype
MGI:5468940

cn6

• Allelic Composition: Cxcl12^tm1Tng/Cxcl12^tm1.1Link; Tg(BGLAP-cre)1Clem/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * C57BL/6NTac * FVB/N

hematopoietic system

hematopoietic system phenotype ( J:193594 )

N • mice exhibit normal hematopoietic stem cell and lymphoid progenitors morphology and physiology

decreased B cell number ( J:193594 )

• in the blood

immune system

decreased B cell number ( J:193594 )

• in the blood